Team:Paris/August 2

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{{Paris/Calendar_Links|August 1 |August 3}}
{{Paris/Calendar_Links|August 1 |August 3}}
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=== Results of the transformation ===
 
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{| border="1"
 
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|align="center"|'''Ligation name'''
 
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|align="center"|'''Description'''
 
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|align="center"|'''Antibio'''
 
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|align="center"|'''Nb colonies observed'''
 
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|align="center"|'''Fluorescence'''
 
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|align="center"|'''Comments'''
 
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|-
 
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|align="center"|L100
 
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|align="center"|
 
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rbs TetR - ECFP
 
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<br>D110 (BV) -  D130 (BI) 
 
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|align="center"|
 
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|align="center"|0
 
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|align="center"|
 
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|align="center"|To do again
 
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|-
 
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|align="center"|L101
 
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|align="center"|
 
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rbs TetR - GFP tripart
 
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<br>D110 (BV) -  D131 (BI) 
 
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|align="center"|
 
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|align="center"|(1)
 
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|align="center"|
 
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|align="center"|To do again
 
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|-
 
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|align="center"|L102
 
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|align="center"|
 
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Strong rbs - YFP
 
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<br>D129 (BV) -  D118 (BI) 
 
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|align="center"|
 
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|align="center"|+/- 100
 
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|align="center"|
 
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|align="center"|To do again
 
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|-
 
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|align="center"|L103
 
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|align="center"|
 
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Strong rbs - GFP
 
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<br>D129 (BV) -  D122 (BI) 
 
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|align="center"|
 
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|align="center"|a lot ++
 
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|align="center"|
 
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|align="center"|To do again
 
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|-
 
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|align="center"|L104
 
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|align="center"|
 
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Strong rbs - lasR activator
 
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<br>D129 (BV) -  D114 (BI) 
 
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|align="center"|
 
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|align="center"|a lot ++
 
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|align="center"|
 
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|align="center"|To do again
 
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|-
 
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|align="center"|L105
 
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|align="center"|
 
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Strong promoter - ECFP
 
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<br>D123 (BV) - D130 (BI)
 
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|align="center"|
 
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|align="center"|a lot ++
 
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|align="center"|
 
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|align="center"|OK
 
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|-
 
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|align="center"|L106
 
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|align="center"|
 
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Strong promoter - gfp Tripart
 
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<br>D123 (BV) - D131 (BI)
 
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|align="center"|
 
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|align="center"|a lot ++
 
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|align="center"|
 
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|align="center"|OK
 
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|-
 
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|align="center"|L107
 
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|align="center"|
 
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Strongest promoter - ECFP
 
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<br>D103 (BV) - D131 (BI)
 
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|align="center"|
 
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|align="center"|a lot ++
 
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|align="center"|
 
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|align="center"|To do again
 
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|-
 
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|align="center"|L108 n°2 (the right one)
 
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|align="center"|
 
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Strong promoter -  gfp Tripart
 
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<br>D103 (BV) - D130 (BI)
 
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|align="center"|
 
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|align="center"|+/- 100
 
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|align="center"|
 
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|align="center"|OK
 
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|-
 
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|align="center"|L109
 
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|align="center"|
 
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Strong promoter -  ecfp
 
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<br>D124 (BV) - D130 (BI)
 
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|align="center"|
 
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|align="center"|+/- 100
 
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|align="center"|
 
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|align="center"|To do again
 
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|-
 
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|align="center"|L110
 
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|align="center"|
 
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Medium promoter - gfp Tripart
 
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<br>D124 (BV) - D131 (BI)
 
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|align="center"|
 
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|align="center"|a lot ++
 
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|align="center"|
 
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|align="center"|OK
 
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|-
 
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|align="center"|L111
 
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|align="center"|
 
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Weak promoter - ECFP
 
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<br>D104 (BV) - D130 (BI)
 
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|align="center"|
 
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|align="center"|a lot ++
 
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|align="center"|
 
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|align="center"|To do again
 
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|-
 
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|align="center"|L112
 
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|align="center"|
 
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Weak promoter - gfp
 
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<br>D104 (BV) - D131 (BI)
 
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|align="center"|
 
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|align="center"|a lot ++
 
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|align="center"|
 
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|align="center"|To do again
 
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|-
 
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|align="center"|L113
 
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|align="center"|
 
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AracpBAD - ecfp
 
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<br>D126 (BV) - D130 (BI)
 
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|align="center"|
 
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|align="center"|1
 
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|align="center"|
 
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|align="center"|To do again
 
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|-
 
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|align="center"|L114
 
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|align="center"|
 
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AracpBAD - gfp tripart
 
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<br>D126 (BV) - D131 (BI)
 
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|align="center"|
 
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|align="center"|2
 
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|align="center"|
 
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|align="center"|To do again
 
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|-
 
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|align="center"|L115
 
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|align="center"|
 
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pLas - ECFP
 
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<br>D105 (BV) - D130 (BI)
 
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|align="center"|
 
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|align="center"|a lot ++
 
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|align="center"|
 
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|align="center"|To do again
 
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|-
 
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|align="center"|L116
 
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|align="center"|
 
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pLas - gfp Tripart
 
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<br>D105 (BV) - D131 (BI)
 
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|align="center"|
 
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|align="center"|a lot ++
 
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|align="center"|
 
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|align="center"|To do again
 
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|-
 
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|align="center"|L117
 
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|align="center"|
 
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yfp - Double Terminator
 
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<br>D117 (FI) - D125 (FV)
 
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|align="center"|
 
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|align="center"|+/- 30
 
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|align="center"|
 
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|align="center"|To do again
 
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|-
 
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|align="center"|L118
 
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|align="center"|
 
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rfp - Double Terminator
 
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<br>D121 (FI) - D125 (FV)
 
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|align="center"|
 
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|align="center"|+/- 30
 
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|align="center"|
 
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|align="center"|To do again
 
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|-
 
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|align="center"|L119
 
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|align="center"|
 
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lasR activator + LVA - Double Terminator
 
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<br>D113 (FI) - D125 (FV)
 
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|align="center"|
 
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|align="center"|+/- 30
 
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|align="center"|
 
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|align="center"|To do again
 
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|-
 
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|align="center"|L120
 
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|align="center"|
 
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tetR repressible - ECFP
 
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<br>D106 (BV) - D130 (BI)
 
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|align="center"|
 
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|align="center"|a lot ++
 
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|align="center"|
 
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|align="center"|To do again
 
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|-
 
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|align="center"|L121
 
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|align="center"|
 
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Strong promoter - gfp tripart
 
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<br>D106 (BV) - D130 (BI)
 
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|align="center"|
 
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|align="center"|a lot ++
 
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|align="center"|
 
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|align="center"|OK
 
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|-
 
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|align="center"|L122
 
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|align="center"|
 
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RBS-lasI - ecfp
 
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<br>D107 (BV) - D130 (BI)
 
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|align="center"|
 
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|align="center"|0
 
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|align="center"|
 
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|align="center"|To do again
 
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|-
 
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|align="center"|L123
 
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|align="center"|
 
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RBS lasI - ecfp
 
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<br>D107 (BV) - D131 (BI)
 
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|align="center"|
 
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|align="center"|1
 
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|align="center"|
 
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|align="center"|To do again
 
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|align="center"|L124
 
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|align="center"|
 
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Strongest RBS - rfp
 
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<br>D102 (BV) - D122 (BI)
 
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|align="center"|
 
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|align="center"|a lot ++
 
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|align="center"|
 
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|align="center"|To do again
 
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|-
 
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|align="center"|L125
 
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|align="center"|
 
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Strongest RBS - yfp
 
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<br>D102 (BV) - D118 (BI)
 
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|align="center"|
 
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|align="center"|a lot ++
 
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|align="center"|
 
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|align="center"|To do again
 
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|-
 
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|align="center"|L126
 
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|align="center"|
 
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Strongest RBS (1)- LacR activator (+LVA)
 
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<br>D102 (BV) - D118 (BI)
 
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|align="center"|
 
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|align="center"|no dish found!!!
 
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|align="center"|
 
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|align="center"|To do again
 
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|align="center"|L127
 
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|align="center"|
 
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gfp (1)- Double terminator
 
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<br>D119 (FI) - D125 (FV)
 
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|align="center"|
 
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|align="center"|no dish found!!!
 
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|align="center"|
 
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|align="center"|To do again
 
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|align="center"|'''Controls'''
 
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|align="center"|
 
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|align="center"|
 
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|align="center"|
 
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|align="center"|
 
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|align="center"|
 
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|align="center"|C1
 
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|align="center"|
 
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D110
 
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|align="center"|
 
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|align="center"|0
 
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|align="center"|
 
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|align="center"|-
 
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|align="center"|C2
 
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|align="center"|
 
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D129
 
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|align="center"|
 
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|align="center"|5
 
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|align="center"|
 
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|align="center"|-
 
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|align="center"|C3
 
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|align="center"|
 
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D123
 
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|align="center"|
 
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|align="center"|+/- 100
 
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|align="center"|
 
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|align="center"|-
 
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|align="center"|C4
 
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|align="center"|
 
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D103
 
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|align="center"|
 
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|align="center"|+/- 20
 
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|align="center"|
 
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|align="center"|-
 
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|align="center"|C5
 
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|align="center"|
 
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D124
 
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|align="center"|
 
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|align="center"|+/- 100
 
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|align="center"|
 
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|align="center"|-
 
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|align="center"|C6
 
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|align="center"|
 
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D104
 
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|align="center"|
 
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|align="center"|+/- 100
 
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|align="center"|
 
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|align="center"|-
 
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|align="center"|C7
 
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|align="center"|
 
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D126
 
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|align="center"|
 
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|align="center"|0
 
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|align="center"|
 
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|align="center"|-
 
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|align="center"|C8
 
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|align="center"|
 
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D105
 
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|align="center"|
 
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|align="center"|+/- 20
 
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|align="center"|
 
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|align="center"|-
 
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|align="center"|C9
 
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|align="center"|
 
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D125
 
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|align="center"|
 
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|align="center"|0
 
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|align="center"|
 
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|align="center"|-
 
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|align="center"|C10
 
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|align="center"|
 
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D106
 
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|align="center"|
 
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|align="center"|0
 
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|align="center"|
 
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|align="center"|-
 
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|align="center"|C11
 
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|align="center"|
 
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D107
 
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|align="center"|
 
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|align="center"|0
 
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|align="center"|
 
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|align="center"|-
 
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|align="center"|C12
 
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|align="center"|
 
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D102
 
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|align="center"|
 
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|align="center"|+/- 10
 
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|align="center"|
 
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|align="center"|-
 
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|align="center"|Positive control
 
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|align="center"|
 
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puc19
 
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|align="center"|155 (transformation efficiency:1.5*10^7/ug)
 
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|align="center"|
 
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|align="center"|
 
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|align="center"|-
 
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|}
 
=== Results of the transformation (bis-the good one to leave in august 2)===
=== Results of the transformation (bis-the good one to leave in august 2)===

Revision as of 18:04, 4 August 2008

← Yesterday

↓ Calendar ↑

Tomorrow →


Results of the transformation (bis-the good one to leave in august 2)

Ligation name Description Antibio Nb colonies observed Fluorescence Comments
L100

rbs TetR - ECFP
D110 (BV) - D130 (BI)

Amp 608 To do again
L101

rbs TetR - GFP tripart
D110 (BV) - D131 (BI)

Amp > 4000 GFP positive OK
L113

AracpBAD - ecfp
D126 (BV) - D130 (BI)

Kana 9 To do again
L114

AracpBAD - gfp tripart
D126 (BV) - D131 (BI)

Kana 9 To do again
L120

tetR repressible promoter - ECFP
D106 (BV) - D130 (BI)

Amp 74 To do again
L122

RBS-lasI - ecfp
D107 (BV) - D130 (BI)

Amp 4 To do again
L123

RBS lasI - ecfp
D107 (BV) - D131 (BI)

Amp > 4000 To do again
L126

Strongest RBS (1)- LasR activator (+LVA)
D102 (BV) - D114 (BI)

Amp 944 To do again
Retrieved from "http://2008.igem.org/Team:Paris/August_2"