Team:Paris/August 21
From 2008.igem.org
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+ | =Screening of the cloning of E0240 and FlhDC+promotor= | ||
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+ | We obtained single colonies with the dilution 100. | ||
+ | <br>13 clones were analysed by PCR | ||
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+ | ==PCR screening== | ||
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+ | reaction mixture (25 ) | ||
+ | PCR screening programm | ||
+ | *elongation time: 1 min 30 | ||
+ | *primers: O18 and O19 |
Revision as of 13:43, 21 August 2008
Construction of pFlgA - YFP tripart (+/- LVA)Aim : Construction of "pFlgA-RBS-YFP-dbl ter" (pFlgA-E0430/E0432) DigestionGel ExtractionProtocol [[Image:KR00019b.jpg| thumb|Gel Extraction of D166-D167]
Screening of the cloning of E0240 and FlhDC+promotorWe obtained single colonies with the dilution 100.
PCR screeningreaction mixture (25 ) PCR screening programm
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