Team:Paris/August 21
From 2008.igem.org
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|colspan="18"|'''Gel n°1 (E0240 in pSB3K3)''' | |colspan="18"|'''Gel n°1 (E0240 in pSB3K3)''' | ||
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+ | |'''well n°''' | ||
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+ | |'''sample''' | ||
+ | |1 kb DNA ladder | ||
+ | |positive control | ||
+ | |negative control | ||
+ | |colspan="13"|S159.1 | ||
+ | |100 bp DNA ladder | ||
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+ | |'''colonie n°''' | ||
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+ | |'''expected size''' | ||
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+ | |colspan="13"| | ||
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+ | |'''measured size''' | ||
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+ | |} | ||
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+ | {|border="1" style="text-align: center" | ||
+ | |colspan="18"|'''Gel n°2 (FlhDC+promotor in pSB1A2)''' | ||
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|'''well n°''' | |'''well n°''' |
Revision as of 13:58, 21 August 2008
Construction of pFlgA - YFP tripart (+/- LVA)Aim : Construction of "pFlgA-RBS-YFP-dbl ter" (pFlgA-E0430/E0432) DigestionGel ExtractionProtocol [[Image:KR00019b.jpg| thumb|Gel Extraction of D166-D167]
Screening of the cloning of E0240 and FlhDC+promotorWe obtained single colonies with the dilution 100.
PCR screeningreaction mixture (25 µL)
PCR screening programm
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