Team:Paris/August 21
From 2008.igem.org
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[[Image:KR000200.jpg|thumb|'''Gel n°1''' (E0240 screening)]] | [[Image:KR000200.jpg|thumb|'''Gel n°1''' (E0240 screening)]] | ||
[[Image:KR000207.jpg|thumb|'''Gel n°2''' (FlhDC+promotor screening)]] | [[Image:KR000207.jpg|thumb|'''Gel n°2''' (FlhDC+promotor screening)]] |
Revision as of 14:14, 21 August 2008
Construction of pFlgA - YFP tripart (+/- LVA)Aim : Construction of "pFlgA-RBS-YFP-dbl ter" (pFlgA-E0430/E0432) DigestionGel ExtractionProtocol [[Image:KR00019b.jpg| thumb|Gel Extraction of D166-D167]
Screening of the cloning of E0240 and FlhDC+promotorWe obtained single colonies with the dilution 100.
PCR screeningreaction mixture (25 µL)
PCR screening programm
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