Team:Paris/August 21
From 2008.igem.org
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==Electrophoresis== | ==Electrophoresis== | ||
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+ | [[Image:KR000200.jpg|thumb|'''Gel n°1''' (E0240 screening)]] | ||
+ | [[Image:KR000207.jpg|thumb|'''Gel n°2''' (FlhDC+promotor screening)]] | ||
*1% agarose gel | *1% agarose gel | ||
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'''Results''': | '''Results''': | ||
*The clone of E0240 (S159.1) always have several bands amplified by PCR. It might contain different plasmids. | *The clone of E0240 (S159.1) always have several bands amplified by PCR. It might contain different plasmids. | ||
*The clone of FlhDC+promotor (S161.1) don't have the correct size band. It also doesn't have the insert in the plasmid. | *The clone of FlhDC+promotor (S161.1) don't have the correct size band. It also doesn't have the insert in the plasmid. |
Revision as of 14:43, 21 August 2008
Construction of pFlgA - YFP tripart (+/- LVA)Aim : Construction of "pFlgA-RBS-YFP-dbl ter" (pFlgA-E0430/E0432) DigestionGel ExtractionProtocol [[Image:KR00019b.jpg| thumb|Gel Extraction of D166-D167]
Screening of the cloning of E0240 and FlhDC+promotorWe obtained single colonies with the dilution 100.
PCR screeningreaction mixture (25 µL)
PCR screening programm
Electrophoresis
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