Team:Paris/August 21
From 2008.igem.org
(Difference between revisions)
(→Electrophoresis) |
(→Electrophoresis) |
||
Line 208: | Line 208: | ||
[[Image:KR000207.jpg|thumb|'''Gel n°2''' (FlhDC+promotor screening)]] | [[Image:KR000207.jpg|thumb|'''Gel n°2''' (FlhDC+promotor screening)]] | ||
<br> | <br> | ||
- | Results: | + | '''Results''': |
*The clone of E0240 (S159.1) always have several bands amplified by PCR. It might contain different plasmids. | *The clone of E0240 (S159.1) always have several bands amplified by PCR. It might contain different plasmids. | ||
*The clone of FlhDC+promotor (S161.1) don't have the correct size band. It also doesn't have the insert in the plasmid. | *The clone of FlhDC+promotor (S161.1) don't have the correct size band. It also doesn't have the insert in the plasmid. |
Revision as of 14:22, 21 August 2008
Construction of pFlgA - YFP tripart (+/- LVA)Aim : Construction of "pFlgA-RBS-YFP-dbl ter" (pFlgA-E0430/E0432) DigestionGel ExtractionProtocol [[Image:KR00019b.jpg| thumb|Gel Extraction of D166-D167]
Screening of the cloning of E0240 and FlhDC+promotorWe obtained single colonies with the dilution 100.
PCR screeningreaction mixture (25 µL)
PCR screening programm
Electrophoresis
|