From 2008.igem.org
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Revision as of 12:12, 25 August 2008
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← Yesterday ↓ Calendar ↑Tomorrow →
Construction of pFlgA - GFP Generator
Aim : Construction of "pFlgA-RBS-GFP-dbl ter" (pFlgA-E0430/E0432)    
Transformation of the ligations we did yesterday
Protocol
| Ligation Name
| Description
| Antibio
|
| L164
| D168 (FV) + D132 (FI)
| Amp
|
| Control L164
| D168
| Amp
|
Construction for Synchronization
Results of the transformation we did yesterday
Number of colonies
| Ligation name
| Description
| Antibio
| Number Colonies observed
| Fluorescence
| Comments
|
| Ligations
|
| L158
| D110(BV) - D131(BI)
rbs-TetR - gfp tripart
| Amp
| -
| -
| to do again
|
| L159
| D125(FV) - D109(FI)
rbs-LasI - double terminator
| Kan
| -
| -
| to do again
|
| L162
| D107(BV) - D163(BI)
rbs-LasI - gfp generator
| Amp
| -
| -
| to do again
|
| L163
| D110(BV) - D163(BI)
rbs-TetR - gfp generator
| Amp
| -
| -
| to do again
|
| Controls
|
| TL158
| D110(BV)
| Amp
| -
| -
| to do again
|
| TL159
| D125(FV)
| Kan
| -
| -
| to do again
|
| TL162
| D107(BV)
| Amp
| -
| -
| to do again
|
| TL163
| D110(BV)
| Amp
| -
| -
| to do again
|
| Positive Control
| pUC19
| Amp
| 358 (efficiency 3.5.10^7)
| No
| OK
|
=> We decided to do again the digestions of the ligation's reactions
|
"
