Team:Paris/August 24

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(Ligation)
 
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='''Construction for synchronization'''=
='''Construction for synchronization'''=
 +
=='''Gel extraction'''==
[[Team:Paris/Notebook/Protocols#Migration after digestion| Protocol]]
[[Team:Paris/Notebook/Protocols#Migration after digestion| Protocol]]
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 +
Gel 1 (no image sorry, we forgot to save it!)
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{|border="1" style="text-align: center"
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|'''Well'''
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|1
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|2
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|3
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|4
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|5
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|6
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|7
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|8
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|9
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|10
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|11
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|12
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|-
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|'''Sample'''
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|1kb ladder
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|MP105.1
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|no sample
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|D107
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|no sample
 +
|D109
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|no sample
 +
|MP106
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|no sample
 +
|D110
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|no sample
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|100pb ladder
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|-
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|'''Expected size (pb)'''
 +
| -
 +
|2764
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| -
 +
|2750<br>14
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| -
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|2056<br>708
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| -
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|2782
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| -
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|2768<br>14
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| -
 +
| -
 +
|-
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|'''Measured size (pb)'''
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| -
 +
|style="background: #cbff7B"| 2800
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| -
 +
|style="background: #cbff7B"| 2750
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| -
 +
|style="background: #cbff7B"| 2000<br>700
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| -
 +
|style="background: #cbff7B"| 2800
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| -
 +
|style="background: #cbff7B"| 2750
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| -
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| -
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|}
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 +
Gel 2
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 +
{|border="1" style="text-align: center"
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|'''Well'''
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|1
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|2
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|3
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|4
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|5
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|6
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|7
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|8
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|9
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|10
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|11
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|12
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|-
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|'''Sample'''
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|no sample
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|1kb ladder
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|MP118.1
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|no sample
 +
|D125
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|no sample
 +
|MP143
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|no sample
 +
|D163
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|no sample
 +
|100pb ladder
 +
|no sample
 +
|-
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|'''Expected size (pb)'''
 +
| -
 +
| -
 +
|3318
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| -
 +
|3303<br>15
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| -
 +
|3322
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| -
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|2432<br>890
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| -
 +
| -
 +
| -
 +
|-
 +
|'''Measured size (pb)'''
 +
| -
 +
| -
 +
|style="background: #cbff7B"| 2900
 +
| -
 +
|style="background: #cbff7B"| 3300
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| -
 +
|style="background: #cbff7B"| 2900
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| -
 +
|style="background: #cbff7B"| 2500<br>900
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| -
 +
| -
 +
| -
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|}
 +
 +
=='''Ligation'''==
 +
[[Team:Paris/Notebook/Protocols#Ligation |Protocol]]
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 +
{|border="1" style="text-align: center"
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|'''Ligation Name'''
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|'''Vector Name'''
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|'''Volume Vector (µL)'''
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|'''Insert'''
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|'''Volume Insert (µL)'''
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|-
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|L159
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|D125
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|6
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|D109
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|3.86
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|-
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|L162
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|D107
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|5
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|D163
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|5.3
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|-
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|L163
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|D110
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|7.5
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|D163
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|5.32
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|-
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|Control L159
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|D125
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|6
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| -
 +
| -
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|-
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|Control L162
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|D107
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|5
 +
| -
 +
| -
 +
|-
 +
|Control L163
 +
|D110
 +
|7.5
 +
| -
 +
| -
 +
|}

Latest revision as of 12:58, 25 August 2008

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Construction for synchronization

Gel extraction

Protocol

Gel 1 (no image sorry, we forgot to save it!)

Well 1 2 3 4 5 6 7 8 9 10 11 12
Sample 1kb ladder MP105.1 no sample D107 no sample D109 no sample MP106 no sample D110 no sample 100pb ladder
Expected size (pb) - 2764 - 2750
14
- 2056
708
- 2782 - 2768
14
- -
Measured size (pb) - 2800 - 2750 - 2000
700
- 2800 - 2750 - -

Gel 2

Well 1 2 3 4 5 6 7 8 9 10 11 12
Sample no sample 1kb ladder MP118.1 no sample D125 no sample MP143 no sample D163 no sample 100pb ladder no sample
Expected size (pb) - - 3318 - 3303
15
- 3322 - 2432
890
- - -
Measured size (pb) - - 2900 - 3300 - 2900 - 2500
900
- - -

Ligation

Protocol

Ligation Name Vector Name Volume Vector (µL) Insert Volume Insert (µL)
L159 D125 6 D109 3.86
L162 D107 5 D163 5.3
L163 D110 7.5 D163 5.32
Control L159 D125 6 - -
Control L162 D107 5 - -
Control L163 D110 7.5 - -