"
Team:Paris/August 26
From 2008.igem.org
(Difference between revisions)
(→Cloning of EnvZ*) |
|||
| Line 1: | Line 1: | ||
{{Paris/Calendar_Links|August 25|August 27}} | {{Paris/Calendar_Links|August 25|August 27}} | ||
| + | |||
| + | =PCR= | ||
| + | |||
| + | * pFlhDC (O111-F / O113-R) | ||
| + | * Gene FlhDC (O134-F / O131-R) | ||
| + | * pFlgB (O102-F / O103-R) | ||
| + | * pFlhB (O108-F / O109-R) | ||
| + | -- | ||
| + | *Program: Gradient | ||
| + | Denaturation : 98°C-5'<br> | ||
| + | Cycling 1 (3X) : 98°C-10''<br> | ||
| + | Gradient 61°C +/-10°C - 30''<br> | ||
| + | 72°C-30''<br> | ||
| + | Cycling 2 (25X) : 98°C-10''<br> | ||
| + | 72°C-30''<br> | ||
| + | Elongation : 72°C-5'<br> | ||
| + | -- | ||
| + | Vf=20µL<br> | ||
| + | H20=13,4µL<br> | ||
| + | Buffer5X=4µL<br> | ||
| + | dNTP=0,4µL<br> | ||
| + | O1=1µL<br> | ||
| + | O2=1µL<br> | ||
| + | Phusion=0,2µL<br> | ||
=Cloning of EnvZ*= | =Cloning of EnvZ*= | ||
Revision as of 10:20, 26 August 2008
PCR
--
Denaturation : 98°C-5' Gradient 61°C +/-10°C - 30 Cycling 2 (25X) : 98°C-10 72°C-30 Elongation : 72°C-5' Cloning of EnvZ*Concentration measurement by Biophotometer
Ligation |
