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Team:Paris/August 26
From 2008.igem.org
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Denaturation : 98°C-5'<br> | Denaturation : 98°C-5'<br> | ||
Cycling 1 (3X) : 98°C-10'' | Cycling 1 (3X) : 98°C-10'' | ||
| - | + |  Gradient 61°C +/-10°C - 30'' | |
72°C-30'' | 72°C-30'' | ||
Cycling 2 (25X) : 98°C-10'' | Cycling 2 (25X) : 98°C-10'' | ||
Revision as of 10:22, 26 August 2008
PCR
Denaturation : 98°C-5' 72°C-30 Cycling 2 (25X) : 98°C-10 72°C-30 Elongation : 72°C-5' Vf=20µL Cloning of EnvZ*Concentration measurement by Biophotometer
Ligation |
