Team:Paris/August 27

From 2008.igem.org

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=Cloning of EnvZ* in pSB1A2=
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==Transformation results==
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{|border="1" style="text-align:center"
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|colspan="3"|'''control'''
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|colspan="3"|'''insert / vector mass ratio'''
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|'''transformation with pUC19'''
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|'''transformation without plasmid'''
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|'''ligation without insert'''
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|'''1,8 / 1'''
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|'''2,4 / 1'''
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|'''3,1 / 1'''
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|'''number of colonies'''
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|many
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|0
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|8
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|2
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Revision as of 10:49, 27 August 2008

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Contents

Construction of pFlhB - mRFP Tripart (LVA+)

Aim : Construction of "pFlhB-RBS-mRFP-dbl ter" (pFlhB-I732078) Part icon regulatory.pngPart icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png We can only do the construction with mRFP Tripart (LVA+) because the stable strain with the Biobricks I732011 (mRFP Tripart LVA-) don't to growth.


Digestion

Measurement of the concentration of D187 purified

Protocol (it's same that for Miniprep)

Digestion Name Concentration (µg/mL) Ratio 260/280
D187

Ligation

Protocol

Ligation Name Vector Name Volume Vector (µL) Insert Volume Insert (µL)
L1 D187 D186
Control L1 D187 - -

Cloning of EnvZ* in pSB1A2

Transformation results

control insert / vector mass ratio
transformation with pUC19 transformation without plasmid ligation without insert 1,8 / 1 2,4 / 1 3,1 / 1
number of colonies many 0 0 8 5 2
Retrieved from "http://2008.igem.org/Team:Paris/August_27"