Team:Paris/August 27
From 2008.igem.org
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+ | ==PCR screening== | ||
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+ | *screening programm | ||
+ | *elongation time: 2 min | ||
+ | *number of cycle: 24 | ||
+ | *total volume reaction: 25 | ||
+ | *primers used: O18 and O19 | ||
+ | *positive control: S158 (pSB3K3) | ||
+ | *negative control: no template |
Revision as of 10:55, 27 August 2008
Construction of pFlhB - mRFP Tripart (LVA+)Aim : Construction of "pFlhB-RBS-mRFP-dbl ter" (pFlhB-I732078) We can only do the construction with mRFP Tripart (LVA+) because the stable strain with the Biobricks I732011 (mRFP Tripart LVA-) don't to growth.
DigestionMeasurement of the concentration of D187 purifiedProtocol (it's same that for Miniprep)
Ligation
Cloning of EnvZ* in pSB1A2Transformation results
PCR screening
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