Team:Paris/August 27
From 2008.igem.org
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===Name of the digestions=== | ===Name of the digestions=== | ||
+ | {|border="1" style="text-align:center" | ||
+ | |- | ||
+ | |'''Name of the digestion''' | ||
+ | |'''Template DNA''' | ||
+ | |'''What's in?''' | ||
+ | |'''Enzymes used''' | ||
+ | |- | ||
+ | |D 188 | ||
+ | |PCR 148 | ||
+ | |OmpR* | ||
+ | |XbaI-PstI | ||
+ | |- | ||
+ | |D 189 | ||
+ | |MP 101.2 | ||
+ | |pSB1A2 | ||
+ | |XbaI-PstI | ||
+ | |} | ||
+ | |||
===Protocol of digestion=== | ===Protocol of digestion=== | ||
===Cleaning of the digestion products=== | ===Cleaning of the digestion products=== |
Revision as of 13:09, 27 August 2008
Construction of pFlhB - mRFP Tripart (LVA+)Aim : Construction of "pFlhB-RBS-mRFP-dbl ter" (pFlhB-I732078) We can only do the construction with mRFP Tripart (LVA+) because the stable strain with the Biobricks I732011 (mRFP Tripart LVA-) don't to growth.
DigestionMeasurement of the concentration of D187 purifiedProtocol (it's same that for Miniprep)
Ligation
Cloning of EnvZ* in pSB1A2Transformation results
PCR screening
Cloning of OmpR*DigestionDetermination of the concentration of DNAWe used the biophotometer
Name of the digestions
Protocol of digestionCleaning of the digestion productsLigationDetermination of the concentration of DNAProtocol of ligation |