Team:Paris/August 27
From 2008.igem.org
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===Determination of the concentration of DNA=== | ===Determination of the concentration of DNA=== | ||
===Protocol of ligation=== | ===Protocol of ligation=== | ||
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+ | * 2 µL Ligase Buffer 10X | ||
+ | * X µL Vector | ||
+ | * Y µL Insert | ||
+ | * Pure water QSP 20 µL | ||
+ | * 1 µL T4 DNA ligase at 400 000 U/mL concentration | ||
+ | * O/N at 16°C |
Revision as of 14:47, 27 August 2008
Construction of pFlhB - mRFP Tripart (LVA+)Aim : Construction of "pFlhB-RBS-mRFP-dbl ter" (pFlhB-I732078) We can only do the construction with mRFP Tripart (LVA+) because the stable strain with the Biobricks I732011 (mRFP Tripart LVA-) don't to growth.
DigestionMeasurement of the concentration of D187 purifiedProtocol (it's same that for Miniprep)
Ligation
Cloning of EnvZ* in pSB1A2Transformation results
PCR screening
Cloning of OmpR*DigestionDetermination of the concentration of DNAWe used the biophotometer
Name of the digestions
Protocol of digestion
Cleaning of the digestion productsLigationDetermination of the concentration of DNAProtocol of ligation
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