Team:Paris/August 28

From 2008.igem.org

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Revision as of 12:28, 29 August 2008

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Contents

Transformation of OmpR*

Construction of pFlhB - mRFP Tripart (LVA+)

Aim : Construction of "pFlhB-RBS-mRFP-dbl ter" (pFlhB-I732078) Part icon regulatory.pngPart icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png We can only do the construction with mRFP Tripart (LVA+) because the stable strain with the Biobricks I732011 (mRFP Tripart LVA-) don't to growth.

Digestion

Measurement of the concentration of D187 purified

Protocol (it's same that for Miniprep)

Digestion Name Concentration (µg/mL) Ratio 260/280
D187

Ligation

Protocol

Ligation Name Vector Name Volume Vector (µL) Insert Volume Insert (µL)
L168 D187 D186
Control L168 D166 -

Cloning of EnvZ*

Digestion

Digestion n° D159 D116
Name PCR147 (EnvZ*) MP108 (lasR-pSB1A2)
volume of DNA digested 3 µL 2 µL
total volume reaction 30 µL 30 µL
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