Team:Paris/August 28

From 2008.igem.org

(Difference between revisions)
(→Cloning of EnvZ*)
(→Cloning of EnvZ*)
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{|border="1" style="text-align: center"
{|border="1" style="text-align: center"
|'''Digestion n°'''
|'''Digestion n°'''
 +
|'''Name'''
 +
|'''volume of DNA digested'''
 +
|'''total volume reaction'''
 +
|'''enzymes used'''
 +
|-
|D159
|D159
 +
|PCR147 (EnvZ*)
 +
|3 µL
 +
|30 µL
 +
|XbaI & PstI
 +
|-
|D116
|D116
-
|-
 
-
|'''Name'''
 
-
|PCR147 (EnvZ*)
 
|MP108 (lasR-pSB1A2)
|MP108 (lasR-pSB1A2)
-
|-
 
-
|'''volume of DNA digested'''
 
-
|3 µL
 
|2 µL
|2 µL
-
|-
 
-
|'''total volume reaction'''
 
-
|30 µL
 
|30 µL
|30 µL
 +
|XbaI & PstI
|}
|}

Revision as of 12:35, 29 August 2008

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Contents

Transformation of OmpR*

Construction of pFlhB - mRFP Tripart (LVA+)

Aim : Construction of "pFlhB-RBS-mRFP-dbl ter" (pFlhB-I732078) Part icon regulatory.pngPart icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png We can only do the construction with mRFP Tripart (LVA+) because the stable strain with the Biobricks I732011 (mRFP Tripart LVA-) don't to growth.

Digestion

Measurement of the concentration of D187 purified

Protocol (it's same that for Miniprep)

Digestion Name Concentration (µg/mL) Ratio 260/280
D187

Ligation

Protocol

Ligation Name Vector Name Volume Vector (µL) Insert Volume Insert (µL)
L168 D187 D186
Control L168 D166 -

Cloning of EnvZ*

Digestion

Digestion n° Name volume of DNA digested total volume reaction enzymes used
D159 PCR147 (EnvZ*) 3 µL 30 µL XbaI & PstI
D116 MP108 (lasR-pSB1A2) 2 µL 30 µL XbaI & PstI
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