Revision as of 18:04, 29 August 2008

Cloning of EnvZ*

defrosing of the cells on ice
adding of 5 µL of ligation products in 100 µL of competent cells in 14 mL falcon polypropylene tubes
30 min on ice
thermal schock of 45 s at 42°C
2 min on ice
adding of 900 µL of SOC
contrifugation 5 min 6000 rpm
removal of 800 µL
plating the 200 µL left on LB + amplicilline
overnight incubation at 37°C

@@ Line 58: / Line 58: @@
 ==Transformation of E. coli DH5 alpha competent cells==
-- defrosing of the cells on ice
+*defrosing of the cells on ice
-<br>- adding of 5 µL of ligation products in 100 µL of competent cells in 14 mL falcon polypropylene tubes
+*adding of 5 µL of ligation products in 100 µL of competent cells in 14 mL falcon polypropylene tubes
-<br>- 30 min on ice
+*30 min on ice
-<br>- thermal schock of 45 s at 42°C
+*thermal schock of 45 s at 42°C
-<br>- 2 min on ice
+*2 min on ice
-<br>- adding of 900 µL of SOC
+*adding of 900 µL of SOC
-<br>- contrifugation 5 min 6000 rpm
+*contrifugation 5 min 6000 rpm
-<br>- removal of 800 µL
+*removal of 800 µL
-<br>- plating the 200 µL left on LB + amplicilline
+*plating the 200 µL left on LB + amplicilline
-<br>- overnight incubation at 37°C
+*overnight incubation at 37°C