Team:Paris/August 7
From 2008.igem.org
(Difference between revisions)
(→Results of the PCR we did last night) |
(→Results of the PCR we did last night) |
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[[image:KR000116.jpg|thumb|Standart PCR to amplify pflgA, pflgB and pflhB(Gel1)]] | [[image:KR000116.jpg|thumb|Standart PCR to amplify pflgA, pflgB and pflhB(Gel1)]] | ||
[[image:KR000117.jpg|thumb|PCR with gradient to amplify pflhDC(Gel2)]] | [[image:KR000117.jpg|thumb|PCR with gradient to amplify pflhDC(Gel2)]] | ||
- | + | '''Electrophoresis settings''' | |
+ | * Gel : 1.5 % agar | ||
+ | * 3µL template DNA | ||
+ | * QuickLoad DNA ladder 100 bp | ||
{|- align = "center" | border="1" | {|- align = "center" | border="1" | ||
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|align="center"|2 | |align="center"|2 | ||
|style="background: #cbff7B"|<center>261 pb</center> | |style="background: #cbff7B"|<center>261 pb</center> | ||
- | |align="center"| | + | |align="center"|250 pb |
|- | |- | ||
|align="center"|PCR_125 | |align="center"|PCR_125 | ||
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|align="center"|3 | |align="center"|3 | ||
|style="background: #cbff7B"|<center>261 pb</center> | |style="background: #cbff7B"|<center>261 pb</center> | ||
- | |align="center"| | + | |align="center"|250 pb |
|- | |- | ||
|align="center"|PCR_126 | |align="center"|PCR_126 | ||
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|align="center"|4 | |align="center"|4 | ||
|style="background: #cbff7B"|<center>260 pb</center> | |style="background: #cbff7B"|<center>260 pb</center> | ||
- | |align="center"| | + | |align="center"|250 pb |
|- | |- | ||
|align="center"|PCR_127 | |align="center"|PCR_127 | ||
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|align="center"|2 to 13 | |align="center"|2 to 13 | ||
|style="background: #ff6d73"|<center>446 pb</center> | |style="background: #ff6d73"|<center>446 pb</center> | ||
- | |align="center"| | + | |align="center"|nothing |
|} | |} | ||
+ | <br><br><br> | ||
+ | '''Results''' | ||
+ | *We have no results for pflhDC, wo don't know yet where is the problem. We will try with other conditions! (yet undetermined) | ||
+ | *Concernaing | ||
==Transformations== | ==Transformations== |
Revision as of 18:50, 7 August 2008
Result of the isolation of coloniesE0240 and pSB3K3E0240 and pSB3K3 are ok : there is a lot of single colonies S120 and S121S120 and S121 : there is a problem, there is nothing on the plates. We have to check whether those strains are really resistant to Amp.
Results of the PCR we did last nightElectrophoresis settings
TransformationsProtocolUse of TOP10 chemically competentcells
List of the Ligation Transformation
PCR Screening of Ligation Transformants of 1st AugustUse of 8 clones of Ligation transformants for screening PCR
Protocol of screening PCR
Conditions of electrophoresis
Results
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