Team:Paris/July 15
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(Difference between revisions)
(New page: {{Paris/Calendar_Links|July 14|July 16}} ==Preparation of Competent Cells== * Dilution 1/200e (1mL of the O/N culture on 200mL of LB without Antiobiotic) * Culture at 37°C until DO=0,46...) |
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* Spin 5min, at 2.000rpm, at RT | * Spin 5min, at 2.000rpm, at RT | ||
* Remove 200µl of the supernatant, and resuspend the pellet in 150µl of the supernatant left | * Remove 200µl of the supernatant, and resuspend the pellet in 150µl of the supernatant left | ||
- | * Spread all the suspension on plates | + | * Spread all the suspension on plates containing adaptate antibiotics |
* Incubate O/N at 37°C | * Incubate O/N at 37°C | ||
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==List of the transformations== | ==List of the transformations== | ||
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|align="center"|No transformation | |align="center"|No transformation | ||
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+ | ==Culture of Cells== | ||
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+ | * Culture of DH5ɑ in 10ml of LB without Antibiotics, O/N at 37°C |
Latest revision as of 10:10, 16 July 2008
Preparation of Competent Cells
DNA Extraction of the Biobricks from the registery 2008
Transformation of the cells
List of the transformations
Culture of Cells
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