Team:Paris/July 25

(Difference between revisions)

Revision as of 10:58, 28 July 2008

Use of Promega's protocol for the extraction of: gel n°1 --> 4 : (D100 --> D110 cl-1 ES))
Use of Qiagen's protocol for the extraction of: gel n°5 --> 8 : (D110 cl-2 ES --> D120))

Test of the succeed of the extraction by electrophoresis on 2µl of the parts extracted.

==> conclusion : we don't succeed to detect DNA by spectrophometry or electrophoresis for the samples produced by Qiagen's protocol.

==> we decide to repeat all the samples from the beginning, so we culture O/N at 37°c all the strains usefull for the experiments

Minipreps : Protocol of Promega has been done on all the clones cultivated on the 24th.

10µl of Miniprep
12.5µl of water
2.5µl of Buffer N°2
0.25µl of BSA 100x
1µl of enzyme 1
1µl of enzyme 2

@@ Line 2: / Line 2: @@
-== Extraction of DNA from gel ==
+== Extraction of the DNA  ==
-Extraction of Minipreps DNA (MP100 --> MP122) from the gel.
+* Use of Promega's protocol for the extraction of: gel n°1 --> 4 : (D100 --> D110 cl-1 ES))
+* Use of Qiagen's protocol for the extraction of: gel n°5 --> 8 : (D110 cl-2 ES --> D120))
-* use of protocol of PROMEGA (gel n°1 --> 4 : (D100 --> D110 cl-1 ES))
+* Test of the succeed of the extraction by electrophoresis on 2µl of the parts extracted.
-* use of protocol of QIAGEN  (gel n°5 --> 8 : (D110 cl-2 ES --> D120))
+==> conclusion : we don't succeed to detect DNA by spectrophometry or electrophoresis for the samples produced by Qiagen's protocol.
+==> we decide to repeat all the samples from the beginning, so we culture O/N at 37°c all the strains usefull for the experiments
 == MiniPreps / Digestion ==