Team:Paris/July 27

From 2008.igem.org

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(Results of extraction : Electrophoresis)
(Replacing page with '{{Paris/Calendar_Links|July 26|July 28}}')
 
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=== Results of extraction : Electrophoresis ===
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{{Paris/Calendar_Links|July 26|July 28}}
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conditions :
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* 10µl of ladder 1 kb
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* 2µl of extraction added with 2µl of loading Dye 6x
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* migration ~30min at 100W
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* Gel 1, 2, 3, 4, 5 = '''0.8%'''   
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* Gel 6 = '''1,5%'''
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gel1 [[Image:080728gel_1.jpg| gel1|200px]]
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gel2 [[Image:080728gel_2.jpg| gel2|200px]]
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gel3 [[Image:080728gel_3.jpg| gel3|200px]]
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{| border="1"
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|align=center|'''Name'''
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|align=center|'''BioBrick'''
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|align=center|'''Tube N°'''
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|align=center|'''Enz 1'''
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|align=center|'''Enz 2'''
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|align=center|'''Obs'''
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|align=center|'''Exp Size'''
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|align=center|'''Mea Size '''
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|align=center|'''Gel'''
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|align=center|'''Band'''
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|-
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! rowspan="5" style="background: #ccccff;" | D100
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! rowspan="5" style="background: #ccccff;" | B0034
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|align=center|1
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! rowspan="2"| XbaI
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! rowspan="2"| PstI
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! rowspan="2"| BI
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! rowspan="2"| 2057 pb
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! rowspan="1"|
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! rowspan="1"|
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! rowspan="1"|
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|-
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|align=center|2
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! rowspan="1"|
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! rowspan="1"|
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|-
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|align=center|3
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|align=center| EcoRI
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|align=center| XbaI
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|align=center| FV
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|align=center| 2076 pb
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|align=center|
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|align=center| 1
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|align=center| 2
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|-
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|align=center|4
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! rowspan="2"| SpeI
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! rowspan="2"| PstI
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! rowspan="2"| BV
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! rowspan="2"| 2077 pb
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! rowspan="2"|
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|align=center| 1
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|align=center| 3
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|-
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|align=center|5
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|align=center| 1
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|align=center| 4
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|-
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! rowspan="2" style="background: #ccccff;" | D101
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! rowspan="2" style="background: #ccccff;" | J23101
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|align=center|1
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! rowspan="2"| SpeI
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! rowspan="2"| PstI
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! rowspan="2"| BV
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! rowspan="2"| 2100 pb
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! rowspan="2"|
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|align=center| 1
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|align=center| 5
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|-
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|align=center|2
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|align=center|
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|align=center|
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|-
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! rowspan="2" style="background: #ccccff;" | D102
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! rowspan="2" style="background: #ccccff;" | J23109
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|align=center|1
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! rowspan="2"| SpeI
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! rowspan="2"| PstI
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! rowspan="2"| BV
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! rowspan="2"| 2100 pb
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! rowspan="2"|
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! rowspan="1"|
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! rowspan="1"|
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|-
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|align=center|2
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! rowspan="1"|
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! rowspan="1"|
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|-
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! rowspan="2" style="background: #ccccff;" | D103
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! rowspan="2" style="background: #ccccff;" | R0079
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|align=center|1
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! rowspan="2"| SpeI
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! rowspan="2"| PstI
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! rowspan="2"| BV
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! rowspan="2"| 2222 pb
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! rowspan="2"|
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|align=center|
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|align=center|
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|-
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|align=center|2
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|align=center|
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|align=center|
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|-
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! rowspan="2" style="background: #ccccff;" | D104
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! rowspan="2" style="background: #ccccff;" | R0040
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|align=center|1
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! rowspan="2"| SpeI
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! rowspan="2"| PstI
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! rowspan="2"| BV
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! rowspan="2"| 2119 pb
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! rowspan="2"|
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|align=center|
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|align=center|
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|-
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|align=center|2
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|align=center|
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|align=center|
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|-
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! rowspan="4" style="background: #ccccff;" | D105
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! rowspan="4" style="background: #ccccff;" | S03154
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|align=center|1
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! rowspan="1"| SpeI
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! rowspan="1"| PstI
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! rowspan="1"| BV
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! rowspan="1"| 2750 pb
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|align=center|
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|align=center|
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|align=center|
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|-
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|align=center|2
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! rowspan="2"| XbaI
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! rowspan="2"| PstI
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! rowspan="2"| BI
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! rowspan="2"| 707 pb
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! rowspan="2"|
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|align=center|
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|align=center|
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|-
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|align=center|3
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|align=center|
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|align=center|
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|-
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|align=center|4
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|align=center|EcoRI
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|align=center|SpeI
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|align=center|FI
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|align=center| 708 pb
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|align=center|
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|align=center|
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|align=center|
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|-
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! rowspan="4" style="background: #ccccff;" | D106
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! rowspan="4" style="background: #ccccff;" | S03879
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|align=center|1
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|align=center|SpeI
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|align=center|PstI
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|align=center|BV
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|align=center| 2768 pb
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|align=center|
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|align=center|
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|align=center|
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|-
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|align=center|2
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! rowspan="2"| XbaI
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! rowspan="2"| PstI
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! rowspan="2"| BI
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! rowspan="2"| 725 pb
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! rowspan="2"|
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|align=center|
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|align=center|
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|-
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|align=center|3
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|align=center|
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|align=center|
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|-
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|align=center|4
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|align=center|EcoRI
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|align=center|SpeI
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|align=center|FI
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|align=center| 726 pb
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|align=center|
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|align=center|
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|align=center|
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|-
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! rowspan="2" style="background: #ccccff;" |D107
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! rowspan="2" style="background: #ccccff;" |C0079
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|align=center| 1
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|align=center|EcoRI
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|align=center|SpeI
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|align=center|FI
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|align=center| 779 pb
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|align=center|
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|align=center|
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|align=center|
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|-
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|align=center| 2
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|align=center|XbaI
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|align=center|PstI
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|align=center|BI
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|align=center| 778 pb
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|align=center|
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|align=center|
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|align=center|
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|-
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! rowspan="2" style="background: #ccccff;"|D108
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! rowspan="2" style="background: #ccccff;"|C0179
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|align=center| 1
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|align=center| EcoRI
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|align=center| SpeI
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|align=center| FI
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|align=center| 746 pb
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|align=center|
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|align=center|
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|align=center|
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|-
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|align=center| 2
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|align=center| XbaI
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|align=center| PstI
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|align=center| BI
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|align=center| 745 pb
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|align=center|
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|align=center|
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|align=center|
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|-
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! rowspan="2" style="background: #ccccff;"|D109
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! rowspan="2" style="background: #ccccff;"|E0030
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|align=center| 1
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|align=center|EcoRI
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|align=center|SpeI
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|align=center|FI
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|align=center| 746 pb
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|align=center|
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|align=center|
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|align=center|
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|-
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|align=center| 2
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|align=center| XbaI
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|align=center| PstI
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|align=center| BI
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|align=center| 745 pb
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|align=center|
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|align=center|
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|align=center|
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|-
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! rowspan="2" style="background: #ccccff;"|D110
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! rowspan="2" style="background: #ccccff;"|E0040
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|align=center| 1
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|align=center| EcoRI
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|align=center| SpeI
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|align=center| FI
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|align=center| 743 pb
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|align=center|
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|align=center|
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|align=center|
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|-
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|align=center| 2
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|align=center| XbaI
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|align=center| PstI
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|align=center| BI
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|align=center| 742 pb
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|align=center|
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|align=center|
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|align=center|
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|-
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! rowspan="2" style="background: #ccccff;"|D111
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! rowspan="2" style="background: #ccccff;"|E1010
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|align=center| 1
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|align=center| EcoRI
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|align=center| SpeI
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|align=center| FI
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|align=center| 704 pb
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|align=center|
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|align=center|
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|align=center|
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|-
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|align=center| 2
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|align=center| XbaI
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|align=center| PstI
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|align=center| BI
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|align=center| 703 pb
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|align=center|
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|align=center|
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|align=center|
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|-
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! rowspan="2" style="background: #ccccff;"|D116
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! rowspan="2" style="background: #ccccff;"|J23100
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|align=center|1
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! rowspan="2"| SpeI
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! rowspan="2"| PstI
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! rowspan="2"| BV
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! rowspan="2"| 2100 pb
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! rowspan="2"|
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! rowspan="1"|
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! rowspan="1"|
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|-
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|align=center|2
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! rowspan="1"|
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! rowspan="1"|
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|-
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! rowspan="2" style="background: #ccccff;"|D117
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! rowspan="2" style="background: #ccccff;"|J23107
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|align=center|1
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! rowspan="2"| SpeI
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! rowspan="2"| PstI
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! rowspan="2"| BV
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! rowspan="2"| 2100 pb
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! rowspan="2"|
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! rowspan="1"|
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! rowspan="1"|
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|-
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|align=center|2
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! rowspan="1"|
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! rowspan="1"|
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|-
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! rowspan="2" style="background: #ccccff;"|D118
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! rowspan="2" style="background: #ccccff;"|B0015
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|align=center|1
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! rowspan="2"|EcoRI
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! rowspan="2"|XbaI
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! rowspan="2"| FV
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! rowspan="2"| 3303 pb
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! rowspan="2"|
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! rowspan="1"|
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! rowspan="1"|
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|-
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|align=center|2
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! rowspan="1"|
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! rowspan="1"|
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|-
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! rowspan="2" style="background: #ccccff;"|D119
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! rowspan="2" style="background: #ccccff;"|I0500
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|align=center|1
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! rowspan="2"|SpeI
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! rowspan="2"|PstI
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! rowspan="2"| FV
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! rowspan="2"| 5621 pb
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! rowspan="2"|
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! rowspan="1"|
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! rowspan="1"|
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|-
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|align=center|2
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! rowspan="1"|
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! rowspan="1"|
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|-
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! rowspan="5" style="background: #ccccff;" | D120
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! rowspan="5" style="background: #ccccff;" | B0030
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|align=center|1
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! rowspan="2"| XbaI
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! rowspan="2"| PstI
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! rowspan="2"| BI
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! rowspan="2"| 37 pb
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! rowspan="2"|
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! rowspan="1"|
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! rowspan="1"|
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|-
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|align=center|2
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! rowspan="1"|
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! rowspan="1"|
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|-
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|align=center|3
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! rowspan="1"|  EcoRI
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! rowspan="1"|  XbaI
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! rowspan="1"|  FV
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! rowspan="1"| 2079 pb
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! rowspan="1"|
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! rowspan="1"|
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! rowspan="1"|
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|-
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|align=center|4
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! rowspan="2"| SpeI
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! rowspan="2"| PstI
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! rowspan="2"| BV
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! rowspan="2"| 2080 pb
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! rowspan="2"|
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! rowspan="1"|
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! rowspan="1"|
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|-
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|align=center|5
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! rowspan="1"|
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! rowspan="1"|
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|-
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! rowspan="3" style="background: #ccccff;"|D121
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! rowspan="3" style="background: #ccccff;"|E0422
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|align=center|1
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! rowspan="3"| XbaI
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! rowspan="3"| PstI
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! rowspan="3"| BI
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! rowspan="3"| 939 pb
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! rowspan="3"|
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! rowspan="1"|
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! rowspan="1"|
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|-
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|align=center|2
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! rowspan="1"|
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! rowspan="1"|
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|-
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|align=center|3
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! rowspan="1"|
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! rowspan="1"|
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|-
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! rowspan="3" style="background: #ccccff;"|D122
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! rowspan="3" style="background: #ccccff;"|E0840
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|align=center|1
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! rowspan="3"| XbaI
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! rowspan="3"| PstI
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! rowspan="3"| BI
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! rowspan="3"| 900 pb
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! rowspan="1"|
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! rowspan="1"|
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! rowspan="1"|
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|-
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|align=center|2
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! rowspan="1"|
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! rowspan="1"|
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|-
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|align=center|3
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! rowspan="1"|
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! rowspan="1"|
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|}
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==> conclusion :
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Latest revision as of 16:10, 28 July 2008

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