Team:Paris/July 29
From 2008.igem.org
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Revision as of 16:21, 5 August 2008
DNA digestion and purificationMix digestionfor each reaction (total volume : 50 µL)
ProtocolEach reaction was :
==> Unfortunately, there were not enough columms, so we took some columms from the QIAGEN MiniPrep kit, hoping that it will work with the QIAquick DNA Gel Extraction kit. Some of the samples were too voluminous, so we separated them into two tubes.
Each of the samples was then analysed by a 1,5% agarose gel:
The ladder used was the 100 bp ladder from New England Biolabs.
List of the digestion
==> Conclusion :The amount of DNA loaded was too low and the DNA ladder used was the wrong one. So this experiment has to be reconducted tomorrow. TransformationsProtocoluse of TOP10 Chemically competent cells
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