Team:Paris/September 1

From 2008.igem.org

(Difference between revisions)
Line 1: Line 1:
{{Paris/Calendar_Links|August 31|September 2}}
{{Paris/Calendar_Links|August 31|September 2}}
 +
=Cloning of EnvZ*=
 +
 +
==Transformation results of L165 (EnvZ*-pSB1A2) from August 29th==
{|border="1" style="text-align: center"
{|border="1" style="text-align: center"
-
|
+
|'''sample'''
-
|'''DNA concentration'''
+
|'''number of colonies'''
-
|'''A260 / A2801'''
+
|-
 +
|positive control pUC19
 +
|many colonies
 +
|-
 +
|transformation negative control
 +
|0
 +
|-
 +
|ligation without insert
 +
|0
 +
|-
 +
|3:1 insert:vector ratio
 +
|5
|-
|-
-
|D159 (EnvZ*)
+
|4:1 insert / vector ratio
-
|9 µg/mL
+
|5
-
|1,59
+
|-
|-
-
|D116 (pSB1A2)
 
-
|13 µg/mL
 
-
|1,29
 
|}
|}

Revision as of 11:36, 1 September 2008

← Yesterday

↓ Calendar ↑

Tomorrow →

Cloning of EnvZ*

Transformation results of L165 (EnvZ*-pSB1A2) from August 29th

sample number of colonies
positive control pUC19 many colonies
transformation negative control 0
ligation without insert 0
3:1 insert:vector ratio 5
4:1 insert / vector ratio 5