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Team:Paris/September 2
From 2008.igem.org
(Difference between revisions)
(→Ligation L173 (rbs-TetR in mRFP-LVA-tripart-pSB1A3)) |
(→Ligation L173 (rbs-TetR in mRFP-LVA-tripart-pSB1A3)) |
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{|border="1" style="text-align: center" | {|border="1" style="text-align: center" | ||
|rowspan="2"| | |rowspan="2"| | ||
| + | |||
|rowspan="2"|'''Control''' | |rowspan="2"|'''Control''' | ||
|colspan="2"|'''Sample''' | |colspan="2"|'''Sample''' | ||
| Line 56: | Line 57: | ||
|13,1 µL | |13,1 µL | ||
|12,6 µL | |12,6 µL | ||
| + | |- | ||
| + | =Ligation L172 ('''rbs-FlhDC''' in '''mRFP-LVA-tripart'''-pSB1A3)= | ||
| + | |||
| + | |||
| + | '''Samples used''' | ||
| + | {|border="1" style="text-align: center" | ||
| + | |'''Name''' | ||
| + | |'''Digestion number''' | ||
| + | |'''Digested by''' | ||
| + | |'''DNA concentration''' | ||
| + | |- | ||
| + | |rbs-FlhDC | ||
| + | |D186 | ||
| + | |EcoRI & SpeI (insert) | ||
| + | |~24 µg/mL | ||
| + | |- | ||
| + | |mRFP-LVA-tripart | ||
| + | |D187 | ||
| + | |EcoRI & XbaI (vector) | ||
| + | |16 µg/mL | ||
|- | |- | ||
|} | |} | ||
| + | <br> | ||
| + | '''Reaction mixtures''' | ||
| + | {|border="1" style="text-align: center" | ||
| + | |rowspan="2"| | ||
| + | |rowspan="2"|'''Control''' | ||
| + | |colspan="2"|'''Sample''' | ||
| + | |- | ||
| + | |'''3:1''' (insert:vector) '''ratio''' | ||
| + | |- | ||
| + | |'''D186''' (insert) | ||
| + | |0 µL | ||
| + | |1 µL | ||
| + | |- | ||
| + | |'''D187''' (vector) | ||
| + | |2,5 µL | ||
| + | |2,5 µL | ||
| + | |} | ||
*Put in the room "Danger azote" at about 16°C at 18h00 (for overnight reaction). | *Put in the room "Danger azote" at about 16°C at 18h00 (for overnight reaction). | ||
Revision as of 16:46, 2 September 2008
Ligation L173 (rbs-TetR in mRFP-LVA-tripart-pSB1A3)Samples used
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