Team:Paris/September 2
From 2008.igem.org
(Difference between revisions)
(→Reaction mixtures (3:1 (insert:vector) ratio)) |
(→Ligation L173) |
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[[Image:Part_icon_rbs.png]][[Image:icon_coding.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]] | [[Image:Part_icon_rbs.png]][[Image:icon_coding.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]] | ||
- | '''Ligation L173''' | + | =='''Ligation L173'''== |
{|border="1" style="text-align: center" | {|border="1" style="text-align: center" | ||
+ | |'''Digestion n°''' | ||
|'''Name''' | |'''Name''' | ||
- | |||
|'''Digested by''' | |'''Digested by''' | ||
|'''DNA concentration''' | |'''DNA concentration''' | ||
|- | |- | ||
- | |||
|D112 | |D112 | ||
+ | |rbs-TetR | ||
|EcoRI & SpeI (insert) | |EcoRI & SpeI (insert) | ||
|~20 µg/mL | |~20 µg/mL | ||
|- | |- | ||
- | |||
|D187 | |D187 | ||
+ | |mRFP-LVA-tripart | ||
|EcoRI & XbaI (vector) | |EcoRI & XbaI (vector) | ||
|16 µg/mL | |16 µg/mL | ||
Line 24: | Line 24: | ||
<br> | <br> | ||
- | '''Reaction mixtures''' | + | ==='''Reaction mixtures'''=== |
{|border="1" style="text-align: center" | {|border="1" style="text-align: center" | ||
|rowspan="2"| | |rowspan="2"| | ||
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* overnight reaction at about 16°C. | * overnight reaction at about 16°C. | ||
<br> | <br> | ||
- | |||
=Construction of rbs-FlhDC-mRFP-LVA-tripart (L172)= | =Construction of rbs-FlhDC-mRFP-LVA-tripart (L172)= | ||
Line 69: | Line 68: | ||
=='''Ligation L172'''== | =='''Ligation L172'''== | ||
{|border="1" style="text-align: center" | {|border="1" style="text-align: center" | ||
+ | |'''Digestion n°''' | ||
|'''Name''' | |'''Name''' | ||
- | |||
|'''Digested by''' | |'''Digested by''' | ||
|'''DNA concentration''' | |'''DNA concentration''' | ||
|- | |- | ||
- | |||
|D186 | |D186 | ||
+ | |rbs-FlhDC | ||
|EcoRI & SpeI (insert) | |EcoRI & SpeI (insert) | ||
|~24 µg/mL | |~24 µg/mL | ||
|- | |- | ||
- | |||
|D187 | |D187 | ||
+ | |mRFP-LVA-tripart | ||
|EcoRI & XbaI (vector) | |EcoRI & XbaI (vector) | ||
|16 µg/mL | |16 µg/mL |
Latest revision as of 19:24, 9 September 2008
Construction of rbs-TetR-mRFP-LVA-tripart (L173)Ligation L173
Reaction mixtures
Construction of rbs-FlhDC-mRFP-LVA-tripart (L172)Ligation L172
Reaction mixtures (3:1 (insert:vector) ratio)
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