Team:Paris/September 4

From 2008.igem.org

(Difference between revisions)
(Transformation results (rbs-TetR in mRFP-LVA-tripart-pSB1A3))
(Transformation results (rbs-TetR in mRFP-LVA-tripart-pSB1A3))
Line 18: Line 18:
|-
|-
|'''Number of colonies'''
|'''Number of colonies'''
-
|
+
|many
-
|
+
|0
-
|
+
|0
-
|
+
|0
-
|
+
|0
|-
|-
|}
|}

Revision as of 12:53, 4 September 2008

← Yesterday

↓ Calendar ↑

Tomorrow →

← Yesterday

↓ Calendar ↑

Tomorrow →

Transformation results (rbs-TetR in mRFP-LVA-tripart-pSB1A3)

Ligation L173

  • insert: D112 (ES) rbs-TetR
  • vector: D187 (EX) mRFP-LVA-tripart-pSB1A3
Sample positive control
pUC19
negative control
no DNA
ligation control
(without insert)
L173 (3:1 ratio) L173 (4:1 ratio)
Number of colonies many 0 0 0 0


L177 = d134 + d187 (pFlhB-mRFP LVA+)

L175 = d149 + d° (pFliL - ECFP LVA+)

L176 = d149 + d° (pFliL - ECFP LVA-)