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Team:Paris/September 4
From 2008.igem.org
(Difference between revisions)
(→Transformation results (rbs-TetR in mRFP-LVA-tripart-pSB1A3)) |
(→Transformation results (rbs-TetR in mRFP-LVA-tripart-pSB1A3)) |
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{{Paris/Calendar_Links|September 3|September 5}} | {{Paris/Calendar_Links|September 3|September 5}} | ||
| - | =Transformation results (rbs-TetR in mRFP-LVA-tripart-pSB1A3)= | + | =PCR Screening of L173 transformants= |
| + | |||
| + | ==Transformation results (rbs-TetR in mRFP-LVA-tripart-pSB1A3)== | ||
Ligation '''L173''' | Ligation '''L173''' | ||
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|} | |} | ||
| + | ==PCR Screening== | ||
| + | |||
| + | PCR screening programm | ||
| + | *elongation tim: 2 min | ||
| + | *primers used: O18 & O19 | ||
| + | *positive control: S158 (pSB3K3) | ||
| + | *negative control: no template | ||
| + | |||
| + | {|border="1" style="text-align: center" | ||
| + | |'''Well n°''' | ||
| + | |1 | ||
| + | |2 | ||
| + | |3 | ||
| + | |4 | ||
| + | |5 | ||
| + | |6 | ||
| + | |7 | ||
| + | |- | ||
| + | |'''Sample''' | ||
| + | |1 kb DNA ladder | ||
| + | |positive PCR control | ||
| + | |negative PCR control | ||
| + | |negative transformation control clone | ||
| + | |L173.1 | ||
| + | |L173.2 | ||
| + | |- | ||
| + | |'''Expected size''' | ||
| + | |colspan="5"| | ||
| + | |colspan="2"|1894 bp | ||
| + | |- | ||
| + | |'''Measured size''' | ||
| + | | | ||
| + | | | ||
| + | | | ||
| + | | | ||
| + | | | ||
| + | | | ||
| + | | | ||
| + | |- | ||
| + | |} | ||
| + | |||
| + | =Transformation results= | ||
L177 = d134 + d187 (pFlhB-mRFP LVA+) | L177 = d134 + d187 (pFlhB-mRFP LVA+) | ||
Revision as of 15:54, 4 September 2008
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