Team:Paris/September 5

From 2008.igem.org

(Difference between revisions)
(→Purification of D112 (rbs-TetR))
(→Purification of D112 (rbs-TetR))
Line 26: Line 26:
Qiaquick Gel Extraction Kit
Qiaquick Gel Extraction Kit
 +
*elution in 30 µL of EB buffer
==Concentration measurement==
==Concentration measurement==

Revision as of 16:02, 5 September 2008

← Yesterday

↓ Calendar ↑

Tomorrow →

Purification of D112 (rbs-TetR)

Electrophoresis and gel extraction

D112 before gel excision
after gel excision

1% agarose gel

Expected size Measured size
pSB1A2 (vector) 2079 bp 2 kb
rbs-TetR (insert) 703 bp 0,7 kb

Qiaquick Gel Extraction Kit

  • elution in 30 µL of EB buffer

Concentration measurement

Sample DNA concentration A260/A280
D112 8 µg/mL 2,31
Retrieved from "http://2008.igem.org/Team:Paris/September_5"