Team:Paris/July 31

From 2008.igem.org

(Difference between revisions)

Revision as of 14:01, 4 August 2008

← Yesterday

↓ Calendar ↑

Tomorrow →

Oligonucleotides design

RBS +

RBS -

Creation of 3 oligonucleotides (O140, O141, O142) in order to create a particular plasmid

Transformation results

Ligation name	Nb of colonies	Description	Volume
L100	0	rbs-TetR <-> ECFP (RBS+LVA+Term)	3+6
L101	(1)	rbs-TetR <-> GFP tripart+strong rbs	3+6
L102	+/- 100	strong RBS (0,6)<-> YFP	5+10
L103	a lot ++	strong RBS (0,6)<-> GFP	5+5
L104	a lot ++	strong RBS (0,6)<-> LasR activator (+LVA)	5+4
L105	a lot ++	strong constitutive promoter <-> ECFP	5+8
L106	a lot ++	strong constitutive promoter <-> GFP tripart	5+8
L107	a lot ++	strongest constitutive promoter <-> ECFP	5+8
L108	+/- 100 (2 dishes!?)	strongest constitutive promoter <-> GFP tripart	5+8
L109	+/- 30 or a lot ++ (2 dishes !?)	medium constitutive promoter <-> ECFP	4+7
L110	a lot ++	medium constitutive promoter <-> gfp tripart	4+7
L111	a lot ++	weak constitutive promoter <-> ECFP	3+5
L112	a lot ++	weak constitutive promoter <-> gfp tripart	3+5
L113	1	Arac pBAD <-> ECFP	5+2.5
L114	2	Arac pBAD <-> gfp tripart	5+2.5
L115	a lot ++	pLas <-> ECFP	7+11
L116	a lot ++	pLas <-> gfp tripart	7+11
L117	+/- 30	Double terminator <-> YFP	4+3
L118	+/- 30	Double terminator <-> mRFP	4+2
L119	+/- 30	Double terminator <-> LasR activator (+LVA)	4+2
L120	a lot ++	tetR repressible promoter <-> ECFP	6+7.5
L121	a lot ++	tetR repressible promoter <-> gfp tripart	6+7.5
L122	0	tetR repble promoter <-> ECFP	6+7.5
L120	a lot ++	tetR repble promoter <-> ECFP	6+7.5
L120	a lot ++	tetR repble promoter <-> ECFP	6+7.5
L120	a lot ++	tetR repble promoter <-> ECFP	6+7.5
L120	a lot ++	tetR repble promoter <-> ECFP	6+7.5
L120	a lot ++	tetR repble promoter <-> ECFP	6+7.5
L120	a lot ++	tetR repble promoter <-> ECFP	6+7.5
2F_SP 1016	E0030	K	a lot

@@ Line 96: / Line 96: @@
 |align="center"|7+11
 |-
-|align="center"|L110
+|align="center"|L116
 |align="center"|a lot ++
 |align="center"|pLas <-> gfp tripart
@@ Line 102: / Line 102: @@
 |-
 |align="center"|L117
-|align="center"|C0040
+|align="center"|+/- 30
-|align="center"|A
+|align="center"|Double terminator <-> YFP
-|align="center"|0 CFU
+|align="center"|4+3
 |-
-|align="center"|1C_SP 1008
+|align="center"|L118
-|align="center"|PSB1A3
+|align="center"|+/- 30
-|align="center"|A
+|align="center"|Double terminator <-> mRFP
-|align="center"|few
+|align="center"|4+2
 |-
-|align="center"|7G_SP 1013
+|align="center"|L119
-|align="center"|E1010
+|align="center"|+/- 30
-|align="center"|K
+|align="center"|Double terminator <-> LasR activator (+LVA)
-|align="center"|medium
+|align="center"|4+2
 |-
-|align="center"|7E_SP 1001
+|align="center"|L120
-|align="center"|E0040
+|align="center"|a lot ++
-|align="center"|A
+|align="center"|tetR repressible promoter <-> ECFP
-|align="center"|2 CFU
+|align="center"|6+7.5
+|-
+|align="center"|L121
+|align="center"|a lot ++
+|align="center"|tetR repressible promoter <-> gfp tripart
+|align="center"|6+7.5
+|-
+|align="center"|L122
+|align="center"|0
+|align="center"|tetR repble promoter <-> ECFP
+|align="center"|6+7.5
+|-
+|align="center"|L120
+|align="center"|a lot ++
+|align="center"|tetR repble promoter <-> ECFP
+|align="center"|6+7.5
+|-
+|align="center"|L120
+|align="center"|a lot ++
+|align="center"|tetR repble promoter <-> ECFP
+|align="center"|6+7.5
+|-
+|align="center"|L120
+|align="center"|a lot ++
+|align="center"|tetR repble promoter <-> ECFP
+|align="center"|6+7.5
+|-
+|align="center"|L120
+|align="center"|a lot ++
+|align="center"|tetR repble promoter <-> ECFP
+|align="center"|6+7.5
+|-
+|align="center"|L120
+|align="center"|a lot ++
+|align="center"|tetR repble promoter <-> ECFP
+|align="center"|6+7.5
+|-
+|align="center"|L120
+|align="center"|a lot ++
+|align="center"|tetR repble promoter <-> ECFP
+|align="center"|6+7.5
 |-
 |align="center"|2F_SP 1016