Team:Paris/July 26

From 2008.igem.org

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== Digestion ==
 
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== Digestion ==
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1µl of enzyme 2<br>
1µl of enzyme 2<br>
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===list of digestion===
 
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{| border="1"
 
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|align="center"|'''Name'''
 
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|align="center"|'''Miniprep'''
 
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|align="center"|'''Biobrick'''
 
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|align="center"|'''Enzyme 1'''
 
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|align="center"|'''Enzyme 2'''
 
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|align="center"|'''Nber tube'''
 
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|-
 
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|align="center"|D100
 
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|align="center"|MP100
 
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|align="center"|B0034
 
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|align="center"|XbaI
 
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|align="center"|PstI
 
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|align="center"|2
 
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|-
 
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|align="center"|
 
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|align="center"|
 
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|align="center"|
 
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|align="center"|EcoRI
 
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|align="center"|XbaI
 
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|align="center"|1
 
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|-
 
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|align="center"|
 
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|align="center"|
 
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|align="center"|
 
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|align="center"|SpeI
 
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|align="center"|PstI
 
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|align="center"|2
 
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|-
 
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|align="center"|D101
 
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|align="center"|MP101
 
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|align="center"|J23101
 
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|align="center"|SpeI
 
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|align="center"|PstI
 
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|align="center"|2
 
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|-
 
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|align="center"|D102
 
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|align="center"|MP102
 
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|align="center"|J23109
 
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|align="center"|SpeI
 
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|align="center"|PstI
 
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|align="center"|2
 
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|-
 
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|align="center"|D103
 
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|align="center"|MP103
 
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|align="center"|R0079
 
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|align="center"|SpeI
 
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|align="center"|PstI
 
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|align="center"|2
 
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|-
 
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|align="center"|D104
 
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|align="center"|MP104
 
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|align="center"|R0040
 
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|align="center"|SpeI
 
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|align="center"|PstI
 
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|align="center"|2
 
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|-
 
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|align="center"|D105
 
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|align="center"|MP105
 
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|align="center"|S03154
 
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|align="center"|SpeI
 
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|align="center"|PstI
 
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|align="center"|1
 
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|-
 
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|align="center"|
 
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|align="center"|
 
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|align="center"|
 
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|align="center"|XbaI
 
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|align="center"|PstI
 
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|align="center"|2
 
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|-
 
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|align="center"|
 
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|align="center"|
 
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|align="center"|
 
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|align="center"|EcoRI
 
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|align="center"|SpeI
 
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|align="center"|1
 
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|-
 
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|align="center"|D106
 
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|align="center"|MP106
 
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|align="center"|J23101
 
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|align="center"|SpeI
 
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|align="center"|PstI
 
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|align="center"|1
 
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|-
 
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|align="center"|
 
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|align="center"|
 
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|align="center"|
 
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|align="center"|XbaI
 
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|align="center"|PstI
 
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|align="center"|2
 
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|-
 
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|align="center"|
 
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|align="center"|
 
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|align="center"|
 
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|align="center"|EcoRI
 
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|align="center"|SpeI
 
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|align="center"|1
 
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|-
 
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|align="center"|D107
 
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|align="center"|MP107
 
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|align="center"|C0079
 
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|align="center"|EcoRI
 
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|align="center"|SpeI
 
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|align="center"|1
 
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|-
 
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|align="center"|D108
 
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|align="center"|MP108
 
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|align="center"|C0179
 
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|align="center"|EcoRI
 
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|align="center"|SpeI
 
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|align="center"|1
 
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|-
 
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|align="center"|D109
 
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|align="center"|MP109
 
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|align="center"|E0030
 
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|align="center"|EcoRI
 
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|align="center"|SpeI
 
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|align="center"|1
 
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|-
 
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|align="center"|D110
 
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|align="center"|MP110
 
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|align="center"|E0040
 
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|align="center"|EcoRI
 
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|align="center"|SpeI
 
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|align="center"|1
 
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|-
 
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|align="center"|D111
 
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|align="center"|MP111
 
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|align="center"|E1010
 
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|align="center"|EcoRI
 
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|align="center"|SpeI
 
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|align="center"|1
 
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|-
 
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|align="center"|D116
 
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|align="center"|MP116
 
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|align="center"|J23100
 
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|align="center"|SpeI
 
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|align="center"|PstI
 
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|align="center"|2
 
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|-
 
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|align="center"|D117
 
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|align="center"|MP117
 
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|align="center"|J23107
 
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|align="center"|SpeI
 
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|align="center"|PstI
 
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|align="center"|2
 
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|-
 
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|align="center"|D118
 
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|align="center"|MP118
 
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|align="center"|B0015
 
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|align="center"|EcoRI
 
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|align="center"|XbaI
 
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|align="center"|2
 
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|-
 
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|align="center"|D119
 
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|align="center"|MP119
 
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|align="center"|I0500
 
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|align="center"|SpeI
 
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|align="center"|PstI
 
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|align="center"|2
 
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|-
 
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|align="center"|D120
 
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|align="center"|MP120
 
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|align="center"|B0030
 
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|align="center"|SpeI
 
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|align="center"|PstI
 
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|align="center"|2
 
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|-
 
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|align="center"|
 
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|align="center"|
 
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|align="center"|
 
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|align="center"|EcoRI
 
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|align="center"|XbaI
 
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|align="center"|1
 
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|-
 
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|align="center"|
 
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|align="center"|
 
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|align="center"|
 
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|align="center"|XbaI
 
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|align="center"|PstI
 
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|align="center"|2
 
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|-
 
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|align="center"|D121
 
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|align="center"|MP121
 
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|align="center"|E0422
 
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|align="center"|XbaI
 
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|align="center"|PstI
 
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|align="center"|3
 
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|-
 
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|align="center"|D122
 
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|align="center"|MP122
 
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|align="center"|E0840
 
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|align="center"|XbaI
 
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|align="center"|PstI
 
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|align="center"|3
 
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|}
 
== Results of digestions : Electrophoresis ==
== Results of digestions : Electrophoresis ==

Revision as of 14:49, 26 July 2008

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Contents

MiniPreps

  • The protocol of MiniPrep of Promega has been done on all the clones cultivated on the 25th.
Name Biobrick Description
MP100 [http://partsregistry.org/Part:BBa_B0034 B0034] Strongest RBS (Efficiency = 1)
MP101 [http://partsregistry.org/Part:BBa_J23101 J23101] Strongest Constitutive promoter
MP102 [http://partsregistry.org/Part:BBa_J23109 J23109] Weak Constitutive promoter
MP103 [http://partsregistry.org/Part:BBa_R0079 R0079] promoter pLas (lasr & PAI regulated)
MP104 [http://partsregistry.org/Part:BBa_R0040 R0040] tetR repressible promoter
MP105 [http://partsregistry.org/Part:BBa_S03154 S03154] B0034(rbs) ->LasI
MP106 [http://partsregistry.org/Part:BBa_S03879 S03879] B0034(rbs) -> TetR
MP107 [http://partsregistry.org/Part:BBa_C0079 C0079] lasR activator with LVA Tag
MP108 [http://partsregistry.org/Part:BBa_C0179 C0179] lasR activator
MP109 [http://partsregistry.org/Part:BBa_E0030 E0030] YFP
MP110 [http://partsregistry.org/Part:BBa_E0040 E0040] GFP
MP111 [http://partsregistry.org/Part:BBa_E1010 E1010] mRFP
MP116 [http://partsregistry.org/Part:BBa_J23100 J23100] Strong constitutive promoter in J61002
MP117 [http://partsregistry.org/Part:BBa_J23107 J23107] Medium constitutive promoter in J61002
MP118 [http://partsregistry.org/Part:BBa_B0015 B0015] Double terminator
MP119 [http://partsregistry.org/Part:BBa_I0500 I0500] AraC pBAD
MP120 [http://partsregistry.org/Part:BBa_B0030 B0030] Strong RBS (Efficiency = 0,6)
MP121 [http://partsregistry.org/Part:BBa_E0422 E0422] ECFP (RBS+LVA+Term)
MP122 [http://partsregistry.org/Part:BBa_E0840 E0840] gfp tri-part; strong rbs


Digestion

Digestion Mix

10µl of Miniprep (clone 1 or 2)
12.5µl of water
2.5µl of Buffer N°2
0.25µl of BSA 100x
1µl of enzyme 1
1µl of enzyme 2


Results of digestions : Electrophoresis

Retrieved from "http://2008.igem.org/Team:Paris/July_26"