Team:Paris/July 31

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[[Image:RBS-.jpg|thumb|RBS -]]
[[Image:RBS-.jpg|thumb|RBS -]]
Creation of 3 oligonucleotides ([[team:Paris/Notebook/Oligo|O140, O141, O142]]) in order to create a particular plasmid
Creation of 3 oligonucleotides ([[team:Paris/Notebook/Oligo|O140, O141, O142]]) in order to create a particular plasmid
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==Transformation results==
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{| border="1"
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|align="center"|'''Ligation name'''
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|align="center"|'''Nb of colonies'''
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|align="center"|'''Description'''
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|align="center"|'''Volume'''
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|-
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|align="center"|L100
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|align="center"|0
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|align="center"|rbs-TetR <-> ECFP (RBS+LVA+Term)
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|align="center"|3+6
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|-
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|align="center"|L101
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|align="center"|(1)
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|align="center"|rbs-TetR <-> GFP tripart+strong rbs
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|align="center"|3+6
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|-
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|align="center"|L102
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|align="center"|+/- 100
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|align="center"|strong RBS (0,6)<-> YFP
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|align="center"|5+10
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|-
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|align="center"|L103
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|align="center"|a lot ++
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|align="center"|strong RBS (0,6)<-> GFP
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|align="center"|5+5
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|-
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|align="center"|L104
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|align="center"|a lot ++
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|align="center"|strong RBS (0,6)<-> LasR activator (+LVA)
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|align="center"|5+4
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|-
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|align="center"|L105
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|align="center"|a lot ++
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|align="center"|strong constitutive promoter <-> ECFP
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|align="center"|5+8
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|-
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|align="center"|L106
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|align="center"|a lot ++
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|align="center"|strong constitutive promoter <-> GFP tripart
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|align="center"|5+8
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|-
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|align="center"|L107
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|align="center"|a lot ++
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|align="center"|strongest constitutive promoter <-> ECFP
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|align="center"|5+8
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|-
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|align="center"|L108
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|align="center"|+/- 100 (2 dishes!?)
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|align="center"|strongest constitutive promoter <-> GFP tripart
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|align="center"|5+8
 +
|-
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|align="center"|L109
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|align="center"|+/- 30 or a lot ++ (2 dishes !?)
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|align="center"|medium constitutive promoter <-> ECFP
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|align="center"|4+7
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|-
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|align="center"|L110
 +
|align="center"|+/- 30 or a lot ++ (2 dishes !?)
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|align="center"|medium constitutive promoter <-> ECFP
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|align="center"|4+7
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|-
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|align="center"|2G_SP 1000
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|align="center"|C0040
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|align="center"|A
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|align="center"|0 CFU
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|-
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|align="center"|1C_SP 1008
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|align="center"|PSB1A3
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|align="center"|A
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|align="center"|few
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|-
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|align="center"|7G_SP 1013
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|align="center"|E1010
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|align="center"|K
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|align="center"|medium
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|-
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|align="center"|7E_SP 1001
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|align="center"|E0040
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|align="center"|A
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|align="center"|2 CFU
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|-
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|align="center"|2F_SP 1016
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|align="center"|E0030
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|align="center"|K
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|align="center"|a lot
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|}

Revision as of 13:03, 4 August 2008

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Oligonucleotides design

RBS +
RBS -

Creation of 3 oligonucleotides (O140, O141, O142) in order to create a particular plasmid


Transformation results

Ligation name Nb of colonies Description Volume
L100 0 rbs-TetR <-> ECFP (RBS+LVA+Term) 3+6
L101 (1) rbs-TetR <-> GFP tripart+strong rbs 3+6
L102 +/- 100 strong RBS (0,6)<-> YFP 5+10
L103 a lot ++ strong RBS (0,6)<-> GFP 5+5
L104 a lot ++ strong RBS (0,6)<-> LasR activator (+LVA) 5+4
L105 a lot ++ strong constitutive promoter <-> ECFP 5+8
L106 a lot ++ strong constitutive promoter <-> GFP tripart 5+8
L107 a lot ++ strongest constitutive promoter <-> ECFP 5+8
L108 +/- 100 (2 dishes!?) strongest constitutive promoter <-> GFP tripart 5+8
L109 +/- 30 or a lot ++ (2 dishes !?) medium constitutive promoter <-> ECFP 4+7
L110 +/- 30 or a lot ++ (2 dishes !?) medium constitutive promoter <-> ECFP 4+7
2G_SP 1000 C0040 A 0 CFU
1C_SP 1008 PSB1A3 A few
7G_SP 1013 E1010 K medium
7E_SP 1001 E0040 A 2 CFU
2F_SP 1016 E0030 K a lot
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