Team:Paris/July 31

From 2008.igem.org

(Difference between revisions)
(→Transformation results)
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|-
|-
|align="center"|L110
|align="center"|L110
-
|align="center"|+/- 30 or a lot ++ (2 dishes !?)
+
|align="center"|a lot ++
-
|align="center"|medium constitutive promoter <-> ECFP
+
|align="center"|medium constitutive promoter <-> gfp tripart
|align="center"|4+7
|align="center"|4+7
|-
|-
-
|align="center"|2G_SP 1000
+
|align="center"|L111
 +
|align="center"|a lot ++
 +
|align="center"|weak constitutive promoter <-> ECFP
 +
|align="center"|3+5
 +
|-
 +
|align="center"|L112
 +
|align="center"|a lot ++
 +
|align="center"|weak constitutive promoter <-> gfp tripart
 +
|align="center"|3+5
 +
|-
 +
|align="center"|L113
 +
|align="center"|1
 +
|align="center"|Arac pBAD <-> ECFP
 +
|align="center"|5+2.5
 +
|-
 +
|align="center"|L114
 +
|align="center"|2
 +
|align="center"|Arac pBAD <-> gfp tripart
 +
|align="center"|5+2.5
 +
|-
 +
|align="center"|L115
 +
|align="center"|a lot ++
 +
|align="center"|pLas <-> ECFP
 +
|align="center"|7+11
 +
|-
 +
|align="center"|L110
 +
|align="center"|a lot ++
 +
|align="center"|pLas <-> gfp tripart
 +
|align="center"|7+11
 +
|-
 +
|align="center"|L117
|align="center"|C0040
|align="center"|C0040
|align="center"|A
|align="center"|A

Revision as of 13:56, 4 August 2008

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Oligonucleotides design

RBS +
RBS -

Creation of 3 oligonucleotides (O140, O141, O142) in order to create a particular plasmid


Transformation results

Ligation name Nb of colonies Description Volume
L100 0 rbs-TetR <-> ECFP (RBS+LVA+Term) 3+6
L101 (1) rbs-TetR <-> GFP tripart+strong rbs 3+6
L102 +/- 100 strong RBS (0,6)<-> YFP 5+10
L103 a lot ++ strong RBS (0,6)<-> GFP 5+5
L104 a lot ++ strong RBS (0,6)<-> LasR activator (+LVA) 5+4
L105 a lot ++ strong constitutive promoter <-> ECFP 5+8
L106 a lot ++ strong constitutive promoter <-> GFP tripart 5+8
L107 a lot ++ strongest constitutive promoter <-> ECFP 5+8
L108 +/- 100 (2 dishes!?) strongest constitutive promoter <-> GFP tripart 5+8
L109 +/- 30 or a lot ++ (2 dishes !?) medium constitutive promoter <-> ECFP 4+7
L110 a lot ++ medium constitutive promoter <-> gfp tripart 4+7
L111 a lot ++ weak constitutive promoter <-> ECFP 3+5
L112 a lot ++ weak constitutive promoter <-> gfp tripart 3+5
L113 1 Arac pBAD <-> ECFP 5+2.5
L114 2 Arac pBAD <-> gfp tripart 5+2.5
L115 a lot ++ pLas <-> ECFP 7+11
L110 a lot ++ pLas <-> gfp tripart 7+11
L117 C0040 A 0 CFU
1C_SP 1008 PSB1A3 A few
7G_SP 1013 E1010 K medium
7E_SP 1001 E0040 A 2 CFU
2F_SP 1016 E0030 K a lot
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