Team:Paris/July 26

From 2008.igem.org

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Revision as of 16:33, 26 July 2008

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Contents

MiniPreps

  • The protocol of MiniPrep of Promega has been done on all the clones cultivated on the 25th.
Name Biobrick Description
MP100 [http://partsregistry.org/Part:BBa_B0034 B0034] Strongest RBS (Efficiency = 1)
MP101 [http://partsregistry.org/Part:BBa_J23101 J23101] Strongest Constitutive promoter
MP102 [http://partsregistry.org/Part:BBa_J23109 J23109] Weak Constitutive promoter
MP103 [http://partsregistry.org/Part:BBa_R0079 R0079] promoter pLas (lasr & PAI regulated)
MP104 [http://partsregistry.org/Part:BBa_R0040 R0040] tetR repressible promoter
MP105 [http://partsregistry.org/Part:BBa_S03154 S03154] B0034(rbs) ->LasI
MP106 [http://partsregistry.org/Part:BBa_S03879 S03879] B0034(rbs) -> TetR
MP107 [http://partsregistry.org/Part:BBa_C0079 C0079] lasR activator with LVA Tag
MP108 [http://partsregistry.org/Part:BBa_C0179 C0179] lasR activator
MP109 [http://partsregistry.org/Part:BBa_E0030 E0030] YFP
MP110 [http://partsregistry.org/Part:BBa_E0040 E0040] GFP
MP111 [http://partsregistry.org/Part:BBa_E1010 E1010] mRFP
MP116 [http://partsregistry.org/Part:BBa_J23100 J23100] Strong constitutive promoter in J61002
MP117 [http://partsregistry.org/Part:BBa_J23107 J23107] Medium constitutive promoter in J61002
MP118 [http://partsregistry.org/Part:BBa_B0015 B0015] Double terminator
MP119 [http://partsregistry.org/Part:BBa_I0500 I0500] AraC pBAD
MP120 [http://partsregistry.org/Part:BBa_B0030 B0030] Strong RBS (Efficiency = 0,6)
MP121 [http://partsregistry.org/Part:BBa_E0422 E0422] ECFP (RBS+LVA+Term)
MP122 [http://partsregistry.org/Part:BBa_E0840 E0840] gfp tri-part; strong rbs


Digestion

Digestion Mix

10µl of Miniprep (clone 1 or 2)
12.5µl of water
2.5µl of Buffer N°2
0.25µl of BSA 100x
1µl of enzyme 1
1µl of enzyme 2


Results of digestions : Electrophoresis

gel1 [[Image:| gel1|200px]] gel2 [[Image:| gel2|200px]] gel3 [[Image:| gel3|200px]] gel4 [[Image:| gel4|200px]]


gel5 [[Image:‎| gel5|200px]] gel6 [[Image:‎| gel6|200px]]



Name BioBrick Tube N° Enz 1 Enz 2 Obs Exp Size Matrix Exp Size BB Mea Size Matrix Mea Size BB Gel Band
D100 B0034 1 XbaI PstI BI 2057 pb 34 pb 6 2
2 6 3
3 EcoRI XbaI FV 2076 pb 15 pb 1 2
4 SpeI PstI BV 2077 pb 14 pb 1 3
5 1 4
D101 J23101 1 SpeI PstI BV 2100 pb 883 pb 1 5
2 1 6
D102 J23109 1 SpeI PstI BV 2100 pb 883 pb 1 7-8
D103 R0079 1 SpeI PstI BV 2222 pb 14 pb 1 9
2 1 10
D104 R0040 1 SpeI PstI BV 2119 pb 14 pb 1 11
2 1 12
D105 S03154 1 SpeI PstI BV 2750 pb 14 pb 2 5
2 XbaI PstI BI 2057 pb 707 pb 2 2
3 2 3
4 EcoRI SpeI FI 2056 pb 708 pb 2 4
D106 S03879 1 SpeI PstI BV 2768 pb 14 pb 2 6
2 XbaI PstI BI 2057 pb 725 pb 2 7
3 2 8
4 EcoRI SpeI FI 2756 pb 726 pb 2 9
D107 C0079 1 EcoRI SpeI FI 4402 pb 779 pb 2 10
2 XbaI PstI BI 4003 pb 778 pb 2 11
D108 C0179 1 EcoRI SpeI FI 4402 pb 746 pb 2 12
2 XbaI PstI BI 4403 pb 745 pb 3 2
D109 E0030 1 EcoRI SpeI FI 3166 pb 746 pb 3 3
2 XbaI PstI BI 3167 pb 745 pb 3 4
D110 E0040 1 EcoRI SpeI FI 2056 pb 743 pb 3 5
2 XbaI PstI BI 2057 pb 742 pb 3 6
D111 E1010 1 EcoRI SpeI FI 4402 pb 704 pb 3 7
2 XbaI PstI BI 4403 pb 703 pb 3 8
D116 J23100 1 SpeI PstI BV 2100 pb 883 pb 3 9
2 3 10
D117 J23107 1 SpeI PstI BV 2100 pb 883 pb 3 11
2 3 12
D118 B0015 1 EcoRI XbaI FV 3303 pb 15 pb 4 2
2 4 3
D119 I0500 1 SpeI PstI FV 5621 pb 14 pb 4 4
2 4 5
D120 B0030 1 XbaI PstI BI 2057 pb 37 pb 6 4
2 6 5
3 EcoRI XbaI FV 2079 pb 15 pb 4 6
4 SpeI PstI BV 2080 pb 14 pb 4 7
5 4 8
D121 E0422 1 XbaI PstI BI 2057 pb 939 pb 4 9
2 4 10
3 4 11
D122 E0840 1 XbaI PstI BI 2057 pb 900 pb
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