Team:Paris/July 31

From 2008.igem.org

(Difference between revisions)
(→Transformation results)
(→Transformation results)
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|align="center"|L122
|align="center"|L122
|align="center"|0
|align="center"|0
-
|align="center"|tetR repble promoter <-> ECFP
+
|align="center"|B0034rbs-LasI <-> ECFP
-
|align="center"|6+7.5
+
|align="center"|7+3.5
|-
|-
-
|align="center"|L120
+
|align="center"|L123
-
|align="center"|a lot ++
+
|align="center"|1
-
|align="center"|tetR repble promoter <-> ECFP
+
|align="center"|B0034rbs-LasI <-> gfp tripart
-
|align="center"|6+7.5
+
|align="center"|7+3.5
|-
|-
-
|align="center"|L120
+
|align="center"|L124
|align="center"|a lot ++
|align="center"|a lot ++
|align="center"|tetR repble promoter <-> ECFP
|align="center"|tetR repble promoter <-> ECFP
|align="center"|6+7.5
|align="center"|6+7.5
|-
|-
-
|align="center"|L120
+
|align="center"|L125
|align="center"|a lot ++
|align="center"|a lot ++
|align="center"|tetR repble promoter <-> ECFP
|align="center"|tetR repble promoter <-> ECFP
|align="center"|6+7.5
|align="center"|6+7.5
|-
|-
-
|align="center"|L120
+
|align="center"|L126
|align="center"|a lot ++
|align="center"|a lot ++
|align="center"|tetR repble promoter <-> ECFP
|align="center"|tetR repble promoter <-> ECFP
|align="center"|6+7.5
|align="center"|6+7.5
|-
|-
-
|align="center"|L120
+
|align="center"|L127
|align="center"|a lot ++
|align="center"|a lot ++
|align="center"|tetR repble promoter <-> ECFP
|align="center"|tetR repble promoter <-> ECFP
|align="center"|6+7.5
|align="center"|6+7.5
-
|-
+
|
-
|align="center"|L120
+
-
|align="center"|a lot ++
+
-
|align="center"|tetR repble promoter <-> ECFP
+
-
|align="center"|6+7.5
+
-
|-
+
-
|align="center"|2F_SP 1016
+
-
|align="center"|E0030
+
-
|align="center"|K
+
-
|align="center"|a lot
+
|}
|}

Revision as of 14:03, 4 August 2008

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Oligonucleotides design

RBS +
RBS -

Creation of 3 oligonucleotides (O140, O141, O142) in order to create a particular plasmid


Transformation results

Ligation name Nb of colonies Description Volume
L100 0 rbs-TetR <-> ECFP (RBS+LVA+Term) 3+6
L101 (1) rbs-TetR <-> GFP tripart+strong rbs 3+6
L102 +/- 100 strong RBS (0,6)<-> YFP 5+10
L103 a lot ++ strong RBS (0,6)<-> GFP 5+5
L104 a lot ++ strong RBS (0,6)<-> LasR activator (+LVA) 5+4
L105 a lot ++ strong constitutive promoter <-> ECFP 5+8
L106 a lot ++ strong constitutive promoter <-> GFP tripart 5+8
L107 a lot ++ strongest constitutive promoter <-> ECFP 5+8
L108 +/- 100 (2 dishes!?) strongest constitutive promoter <-> GFP tripart 5+8
L109 +/- 30 or a lot ++ (2 dishes !?) medium constitutive promoter <-> ECFP 4+7
L110 a lot ++ medium constitutive promoter <-> gfp tripart 4+7
L111 a lot ++ weak constitutive promoter <-> ECFP 3+5
L112 a lot ++ weak constitutive promoter <-> gfp tripart 3+5
L113 1 Arac pBAD <-> ECFP 5+2.5
L114 2 Arac pBAD <-> gfp tripart 5+2.5
L115 a lot ++ pLas <-> ECFP 7+11
L116 a lot ++ pLas <-> gfp tripart 7+11
L117 +/- 30 Double terminator <-> YFP 4+3
L118 +/- 30 Double terminator <-> mRFP 4+2
L119 +/- 30 Double terminator <-> LasR activator (+LVA) 4+2
L120 a lot ++ tetR repressible promoter <-> ECFP 6+7.5
L121 a lot ++ tetR repressible promoter <-> gfp tripart 6+7.5
L122 0 B0034rbs-LasI <-> ECFP 7+3.5
L123 1 B0034rbs-LasI <-> gfp tripart 7+3.5
L124 a lot ++ tetR repble promoter <-> ECFP 6+7.5
L125 a lot ++ tetR repble promoter <-> ECFP 6+7.5
L126 a lot ++ tetR repble promoter <-> ECFP 6+7.5
L127 a lot ++ tetR repble promoter <-> ECFP 6+7.5
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