Team:Paris/July 31

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Oligonucleotides design

RBS +
RBS -

Creation of 3 oligonucleotides (O140, O141, O142) in order to create a particular plasmid


Transformation results

Ligation name Nb of colonies Description Volume
L100 0 rbs-TetR <-> ECFP (RBS+LVA+Term) 3+6
L101 (1) rbs-TetR <-> GFP tripart+strong rbs 3+6
L102 +/- 100 strong RBS (0,6)<-> YFP 5+10
L103 a lot ++ strong RBS (0,6)<-> GFP 5+5
L104 a lot ++ strong RBS (0,6)<-> LasR activator (+LVA) 5+4
L105 a lot ++ strong constitutive promoter <-> ECFP 5+8
L106 a lot ++ strong constitutive promoter <-> GFP tripart 5+8
L107 a lot ++ strongest constitutive promoter <-> ECFP 5+8
L108 +/- 100 (2 dishes!?) strongest constitutive promoter <-> GFP tripart 5+8
L109 +/- 30 or a lot ++ (2 dishes !?) medium constitutive promoter <-> ECFP 4+7
L110 +/- 30 or a lot ++ (2 dishes !?) medium constitutive promoter <-> ECFP 4+7
2G_SP 1000 C0040 A 0 CFU
1C_SP 1008 PSB1A3 A few
7G_SP 1013 E1010 K medium
7E_SP 1001 E0040 A 2 CFU
2F_SP 1016 E0030 K a lot
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