Ligation name
| Nb of colonies
| Description
| Volume
|
L100
| 0
| rbs-TetR <-> ECFP (RBS+LVA+Term)
| 3+6
|
L101
| (1)
| rbs-TetR <-> GFP tripart+strong rbs
| 3+6
|
L102
| +/- 100
| strong RBS (0,6)<-> YFP
| 5+10
|
L103
| a lot ++
| strong RBS (0,6)<-> GFP
| 5+5
|
L104
| a lot ++
| strong RBS (0,6)<-> LasR activator (+LVA)
| 5+4
|
L105
| a lot ++
| strong constitutive promoter <-> ECFP
| 5+8
|
L106
| a lot ++
| strong constitutive promoter <-> GFP tripart
| 5+8
|
L107
| a lot ++
| strongest constitutive promoter <-> ECFP
| 5+8
|
L108
| +/- 100 (2 dishes!?)
| strongest constitutive promoter <-> GFP tripart
| 5+8
|
L109
| +/- 30 or a lot ++ (2 dishes !?)
| medium constitutive promoter <-> ECFP
| 4+7
|
L110
| a lot ++
| medium constitutive promoter <-> gfp tripart
| 4+7
|
L111
| a lot ++
| weak constitutive promoter <-> ECFP
| 3+5
|
L112
| a lot ++
| weak constitutive promoter <-> gfp tripart
| 3+5
|
L113
| 1
| Arac pBAD <-> ECFP
| 5+2.5
|
L114
| 2
| Arac pBAD <-> gfp tripart
| 5+2.5
|
L115
| a lot ++
| pLas <-> ECFP
| 7+11
|
L116
| a lot ++
| pLas <-> gfp tripart
| 7+11
|
L117
| +/- 30
| Double terminator <-> YFP
| 4+3
|
L118
| +/- 30
| Double terminator <-> mRFP
| 4+2
|
L119
| +/- 30
| Double terminator <-> LasR activator (+LVA)
| 4+2
|
L120
| a lot ++
| tetR repressible promoter <-> ECFP
| 6+7.5
|
L121
| a lot ++
| tetR repressible promoter <-> gfp tripart
| 6+7.5
|
L122
| 0
| B0034rbs-LasI <-> ECFP
| 7+3.5
|
L123
| 1
| B0034rbs-LasI <-> gfp tripart
| 7+3.5
|
L124
| a lot ++
| tetR repble promoter <-> ECFP
| 6+7.5
|
L125
| a lot ++
| tetR repble promoter <-> ECFP
| 6+7.5
|
L126
| a lot ++
| tetR repble promoter <-> ECFP
| 6+7.5
|
L127
| a lot ++
| tetR repble promoter <-> ECFP
| 6+7.5
|
|