User contributions
From 2008.igem.org
- 02:45, 30 October 2008 (diff | hist) Team:Waterloo/Notebook/Oligos
- 02:42, 30 October 2008 (diff | hist) Team:Waterloo/Notebook/Oligos
- 02:40, 30 October 2008 (diff | hist) Team:Waterloo/Notebook/Oligos
- 02:38, 30 October 2008 (diff | hist) Team:Waterloo/Notebook/Oligos
- 02:35, 30 October 2008 (diff | hist) Team:Waterloo/Parts
- 02:34, 30 October 2008 (diff | hist) Team:Waterloo/Parts
- 02:31, 30 October 2008 (diff | hist) N File:PmeIR.PNG (top)
- 02:31, 30 October 2008 (diff | hist) N File:Prfp.PNG (top)
- 02:31, 30 October 2008 (diff | hist) N File:POCI rfp.PNG (top)
- 02:31, 30 October 2008 (diff | hist) N File:PrecA rfp.PNG (top)
- 02:30, 30 October 2008 (diff | hist) N File:C0052R.PNG (top)
- 02:30, 30 October 2008 (diff | hist) Team:Waterloo/Parts
- 02:29, 30 October 2008 (diff | hist) N File:C0051R.PNG (top)
- 02:28, 30 October 2008 (diff | hist) N File:RfpR.PNG (top)
- 02:28, 30 October 2008 (diff | hist) Team:Waterloo/Parts
- 01:56, 30 October 2008 (diff | hist) N File:RPlacTT.PNG (top)
- 01:56, 30 October 2008 (diff | hist) Team:Waterloo/Parts
- 01:51, 30 October 2008 (diff | hist) File:RTTPOCI.PNG (uploaded a new version of "Image:RTTPOCI.PNG") (top)
- 01:51, 30 October 2008 (diff | hist) N File:RTTPOCI.PNG
- 01:50, 30 October 2008 (diff | hist) Team:Waterloo/Parts
- 01:11, 30 October 2008 (diff | hist) File:Headless Chicken.PNG (uploaded a new version of "Image:Headless Chicken.PNG") (top)
- 01:10, 30 October 2008 (diff | hist) N File:Headless Chicken.PNG
- 01:09, 30 October 2008 (diff | hist) Team:Waterloo/Team
- 01:08, 30 October 2008 (diff | hist) Team:Waterloo/Sponsors
- 01:06, 30 October 2008 (diff | hist) N File:EngSoc.PNG (top)
- 01:05, 30 October 2008 (diff | hist) Team:Waterloo/Sponsors
- 01:05, 30 October 2008 (diff | hist) Team:Waterloo/Sponsors
- 20:47, 29 October 2008 (diff | hist) N Waterloo/30 September 2008 (New page: <u>08/09/30 Tue</u><br> [x] Ligation for C0051'' Plac XO, C0051'', and rfp' XO into pJET<br> [x] Transformation of above ligation<br> [x] Check gel of 09/29 GEx<br> [x] PCR of rfp'+TT<b...) (top)
- 20:42, 29 October 2008 (diff | hist) N Waterloo/29 September 2008 (New page: <u>08/09/29 Mon</u><br> [x] Digest to subclone into pSB2K3<br> [x] Gel<br> [x] Gel extract<br>) (top)
- 20:37, 29 October 2008 (diff | hist) Waterloo/27 September 2008 (top)
- 20:37, 29 October 2008 (diff | hist) N Waterloo/27 September 2008 (New page: <u>08/09/27 Sat</u> [x] check gel of PCR from 09/26 [x] Miniprep PCR products)
- 20:33, 29 October 2008 (diff | hist) N Waterloo/26 September 2008 (New page: <u>08/09/26 Fri</u> <br> [x] Colony PCR of C0051'' and PmeIGene6 patches<br> [x] Make competent cells (TG1)<br>) (top)
- 20:29, 29 October 2008 (diff | hist) N Waterloo/25 September 2008 (New page: <u>08/09/25 Thu</u><br> [x] Inoculation of lasR, C0051'', PmeI' Gene6'<br> [x] Miniprep lasR to get pSB2K3<br> [x] Full digest (E/S) of Gene6', PmeI', C0051', pSB2K3(lasR)<br> [x] Gel of ...) (top)
- 20:24, 29 October 2008 (diff | hist) Waterloo/24 September 2008 (top)
- 20:23, 29 October 2008 (diff | hist) N Waterloo/24 September 2008 (New page: <u>08/09/24 Wed</u> [x] Test gel of 09/23 PCR [x] GEx of 09/23 PCR)
- 20:21, 29 October 2008 (diff | hist) N Waterloo/23 September 2008 (New page: <u>08/09/23</u> <br> [x] Ligation of 09/21 GEx<br> [x] Transformation<br> [x] XO PCR to construct Plac+C0051'', C0051'', rfp''<br> [x] Inoculation of stuff to send for sequencing<br>) (top)
- 20:13, 29 October 2008 (diff | hist) N Waterloo/21 September 2008 (New page: <u>08/09/21 Sun</u><br> [x] Gel of XO PCR from 09/20<br> [x] Excise good bands<br>) (top)
- 20:11, 29 October 2008 (diff | hist) N Waterloo/20 September 2008 (New page: <u>08/09/20 Sat</u> [x] XO PCR to make PmeI'+T7Gene6' and C0051'') (top)
- 20:08, 29 October 2008 (diff | hist) N Waterloo/22 September 2008 (New page: <u>08/09/22 Mon</u><br> [x] Colony PCR of pJET clones<br> [x] Run gel of colony PCR<br> [x] GEx of 9/21 gel<br> [x] Transformation<br>) (top)
- 20:01, 29 October 2008 (diff | hist) N Waterloo/19 September 2008 (New page: <u>08/09/19 Fri</u> <br> [x] Check gel of Plac PCRs on 1.2% gel to see if it is separable from primer cloud<br> [x] Gel extraction<br> [x] Ligation into pJET<br> [x] Transformation of pJE...) (top)
- 19:36, 29 October 2008 (diff | hist) Waterloo/29 October 2008 (top)
- 19:35, 29 October 2008 (diff | hist) N Waterloo/29 October 2008 (New page: <u>08/10/29 Wed</u> [] inoculate (in duplicate) all parts with good sequences to make stock [] make glycerol stock of all good parts - to be done at a later date (after inoculation) [] wo...)
- 19:32, 29 October 2008 (diff | hist) N Waterloo/28 October 2008 (New page: <u>08/10/28 Tue</u><br> [x] ship parts to MIT!<br> [] inoculate (in duplicate) all parts with good sequences to make stock<br> - will be done at a later date<br> [x] autoclave liquid wast...) (top)
- 19:23, 29 October 2008 (diff | hist) Waterloo/27 October 2008 (top)
- 19:23, 29 October 2008 (diff | hist) N Waterloo/27 October 2008 (New page: <u>08/10/27 Mon</u> Submissions must be POSTMARKED by the 29th! [x] Prepare samples for submission [x] check sequencing results!! [x] make more liquid LB media in 5 mL tubes [x] work on ...)
- 19:19, 29 October 2008 (diff | hist) Waterloo/26 October 2008 (top)
- 19:19, 29 October 2008 (diff | hist) N Waterloo/26 October 2008 (New page: <u>08/10/26 Sun</u> [x] subculture inoculations from Friday [x] inoculation of PrecA-rfp' transformaion in TG1)
- 19:16, 29 October 2008 (diff | hist) Waterloo/24 October 2008 (top)
- 19:15, 29 October 2008 (diff | hist) N Waterloo/24 October 2008 (New page: <u>08/10/24 Fri</u> [] pestering Scott/neils about protocols for plate reader [] based on sequencing results, prepare samples to ship on Monday (including putative PrecA-rfp' preps!) http...)
- 17:58, 29 October 2008 (diff | hist) Team:Waterloo (→Gold Level Support Provided By)
- 17:56, 29 October 2008 (diff | hist) Team:Waterloo
- 17:54, 29 October 2008 (diff | hist) Team:Waterloo
- 17:49, 29 October 2008 (diff | hist) Team:Waterloo
- 17:26, 29 October 2008 (diff | hist) Team:Waterloo/Notebook/Oligos
- 16:50, 29 October 2008 (diff | hist) Team:Waterloo/Notebook/Oligos
- 16:48, 29 October 2008 (diff | hist) Team:Waterloo/Notebook/Oligos
- 16:38, 29 October 2008 (diff | hist) N File:Robyn Halbot.PNG (top)
- 16:36, 29 October 2008 (diff | hist) N Team:Waterloo/Notebook/Oligos (New page: {| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center" !align="center"|Home !align="cent...)
- 16:34, 29 October 2008 (diff | hist) Team:Waterloo/Notebook (→Oligos)
- 16:33, 29 October 2008 (diff | hist) Team:Waterloo/Notebook
- 15:36, 29 October 2008 (diff | hist) N File:Erica Tiberia.PNG (top)
- 14:31, 29 October 2008 (diff | hist) Team:Waterloo/Team
- 14:25, 29 October 2008 (diff | hist) N File:Jordan LaPointe.PNG (top)
- 14:23, 29 October 2008 (diff | hist) N File:Ben Ho.PNG (top)
- 14:21, 29 October 2008 (diff | hist) N File:Maria Trainer.PNG (top)
- 14:16, 29 October 2008 (diff | hist) N File:Scott Clark.PNG (top)
- 14:11, 29 October 2008 (diff | hist) File:John Heil.PNG (uploaded a new version of "Image:John Heil.PNG") (top)
- 14:09, 29 October 2008 (diff | hist) N File:Nick Fischer.PNG (top)
- 14:07, 29 October 2008 (diff | hist) N File:John Heil.PNG
- 13:59, 29 October 2008 (diff | hist) N File:Jostenn Herritt.PNG (top)
- 13:53, 29 October 2008 (diff | hist) N File:Somil Bhargava.PNG (top)
- 13:34, 29 October 2008 (diff | hist) N File:Hillary Yeung.PNG (top)
- 13:32, 29 October 2008 (diff | hist) Team:Waterloo/Team
- 13:29, 29 October 2008 (diff | hist) Team:Waterloo/Team
- 13:25, 29 October 2008 (diff | hist) N File:Andre Masella.PNG (top)
- 13:24, 29 October 2008 (diff | hist) Team:Waterloo/Team
- 01:14, 29 October 2008 (diff | hist) Team:Waterloo/Sponsors
- 23:28, 28 October 2008 (diff | hist) Team:Waterloo/Sponsors
- 23:27, 28 October 2008 (diff | hist) N File:DeptAppMath.PNG (top)
- 23:25, 28 October 2008 (diff | hist) N File:WEEF.PNG (top)
- 23:24, 28 October 2008 (diff | hist) N File:Sarstedt.PNG (top)
- 23:24, 28 October 2008 (diff | hist) N File:FisherSci.PNG (top)
- 23:20, 28 October 2008 (diff | hist) Team:Waterloo/Sponsors
- 22:55, 28 October 2008 (diff | hist) Team:Waterloo/Notebook/Protocols/Competent Cells (top)
- 22:54, 28 October 2008 (diff | hist) Team:Waterloo/Notebook/Protocols/Transformation (top)
- 22:54, 28 October 2008 (diff | hist) Team:Waterloo/Notebook/Protocols/Inoculation (top)
- 22:54, 28 October 2008 (diff | hist) Team:Waterloo/Notebook/Protocols/Glycerol Stock Preparation (top)
- 22:54, 28 October 2008 (diff | hist) Team:Waterloo/Notebook/Protocols/Ligation (top)
- 22:53, 28 October 2008 (diff | hist) Team:Waterloo/Notebook/Protocols/Gel Electrophoresis (top)
- 22:53, 28 October 2008 (diff | hist) Team:Waterloo/Notebook/Protocols/DNA Extraction (top)
- 22:52, 28 October 2008 (diff | hist) Team:Waterloo/Notebook/Protocols/DNA Digestion (top)
- 22:51, 28 October 2008 (diff | hist) Team:Waterloo/Notebook/Protocols/Colony PCR (top)
- 22:51, 28 October 2008 (diff | hist) Team:Waterloo/Notebook/Recipes (top)
- 22:51, 28 October 2008 (diff | hist) Team:Waterloo/Notebook/Protocols/Miniprep (top)
- 22:50, 28 October 2008 (diff | hist) Team:Waterloo/Notebook/Daily Log (top)
- 22:50, 28 October 2008 (diff | hist) Team:Waterloo/Notebook/Daily Log
- 22:37, 28 October 2008 (diff | hist) N File:Brendan McConkey.PNG (top)
- 22:36, 28 October 2008 (diff | hist) N File:Josh Neufeld.PNG (top)
- 22:34, 28 October 2008 (diff | hist) N File:Marc Aucoin.PNG (top)
- 22:33, 28 October 2008 (diff | hist) N File:Trevor Charles.PNG (top)
- 22:31, 28 October 2008 (diff | hist) N File:Barb Moffatt.PNG (top)
- 22:29, 28 October 2008 (diff | hist) N File:Brian Ingalls.PNG (top)
- 22:25, 28 October 2008 (diff | hist) Team:Waterloo/Notebook/Daily Log
- 22:25, 28 October 2008 (diff | hist) Team:Waterloo/Notebook
- 22:24, 28 October 2008 (diff | hist) Team:Waterloo/Notebook (→Daily Log)
- 22:23, 28 October 2008 (diff | hist) Team:Waterloo/Notebook/Daily Log
- 22:22, 28 October 2008 (diff | hist) N Team:Waterloo/Notebook/Daily Log (New page: <b><u>Lab Calendar</u></b> <center> <tr><td> {{#calendar: title=Waterloo |year=2008 | month=05}} <td><td> {{#calendar: title=Waterloo |year=2008 | month=06}} <td><td> {{#cal...)
- 22:21, 28 October 2008 (diff | hist) Team:Waterloo/Notebook
- 21:26, 28 October 2008 (diff | hist) Team:Waterloo/Notebook/Recipes
- 21:22, 28 October 2008 (diff | hist) Team:Waterloo/Notebook/Recipes
- 21:22, 28 October 2008 (diff | hist) Team:Waterloo/Notebook/Recipes
- 21:20, 28 October 2008 (diff | hist) Team:Waterloo/Notebook/Recipes
- 21:18, 28 October 2008 (diff | hist) Team:Waterloo/Notebook/Recipes
- 21:17, 28 October 2008 (diff | hist) Team:Waterloo/Notebook/Making SOB Media (Removing all content from page) (top)
- 21:17, 28 October 2008 (diff | hist) Team:Waterloo/Notebook/Recipes
- 21:16, 28 October 2008 (diff | hist) Team:Waterloo/Notebook/Making LB Media (Removing all content from page) (top)
- 21:16, 28 October 2008 (diff | hist) Team:Waterloo/Notebook/Recipes
- 21:15, 28 October 2008 (diff | hist) Team:Waterloo/Notebook/Making AI Media (Removing all content from page) (top)
- 21:14, 28 October 2008 (diff | hist) N Team:Waterloo/Notebook/Recipes (New page: * Making Auto Induction Media * Making LB Media * [[Team:Waterloo/Notebook/Making SOB Media|Making SOB...)
- 21:13, 28 October 2008 (diff | hist) Team:Waterloo/Notebook
- 21:08, 28 October 2008 (diff | hist) N Team:Waterloo/Notebook/Competent Cells (Team:Waterloo/Notebook/Competent Cells moved to Team:Waterloo/Notebook/Protocols/Competent Cells) (top)
- 21:08, 28 October 2008 (diff | hist) m Team:Waterloo/Notebook/Protocols/Competent Cells (Team:Waterloo/Notebook/Competent Cells moved to Team:Waterloo/Notebook/Protocols/Competent Cells)
- 21:07, 28 October 2008 (diff | hist) N Team:Waterloo/Notebook/Transformation (Team:Waterloo/Notebook/Transformation moved to Team:Waterloo/Notebook/Protocols/Transformation) (top)
- 21:07, 28 October 2008 (diff | hist) m Team:Waterloo/Notebook/Protocols/Transformation (Team:Waterloo/Notebook/Transformation moved to Team:Waterloo/Notebook/Protocols/Transformation)
- 21:06, 28 October 2008 (diff | hist) N Team:Waterloo/Notebook/Inoculation (Team:Waterloo/Notebook/Inoculation moved to Team:Waterloo/Notebook/Protocols/Inoculation) (top)
- 21:06, 28 October 2008 (diff | hist) m Team:Waterloo/Notebook/Protocols/Inoculation (Team:Waterloo/Notebook/Inoculation moved to Team:Waterloo/Notebook/Protocols/Inoculation)
- 21:06, 28 October 2008 (diff | hist) N Team:Waterloo/Notebook/Glycerol Stock Preparation (Team:Waterloo/Notebook/Glycerol Stock Preparation moved to Team:Waterloo/Notebook/Protocols/Glycerol Stock Preparation) (top)
- 21:06, 28 October 2008 (diff | hist) m Team:Waterloo/Notebook/Protocols/Glycerol Stock Preparation (Team:Waterloo/Notebook/Glycerol Stock Preparation moved to Team:Waterloo/Notebook/Protocols/Glycerol Stock Preparation)
- 21:05, 28 October 2008 (diff | hist) N Team:Waterloo/Notebook/Ligation (Team:Waterloo/Notebook/Ligation moved to Team:Waterloo/Notebook/Protocols/Ligation) (top)
- 21:05, 28 October 2008 (diff | hist) m Team:Waterloo/Notebook/Protocols/Ligation (Team:Waterloo/Notebook/Ligation moved to Team:Waterloo/Notebook/Protocols/Ligation)
- 21:04, 28 October 2008 (diff | hist) N Team:Waterloo/Notebook/Gel Electrophoresis (Team:Waterloo/Notebook/Gel Electrophoresis moved to Team:Waterloo/Notebook/Protocols/Gel Electrophoresis) (top)
- 21:04, 28 October 2008 (diff | hist) m Team:Waterloo/Notebook/Protocols/Gel Electrophoresis (Team:Waterloo/Notebook/Gel Electrophoresis moved to Team:Waterloo/Notebook/Protocols/Gel Electrophoresis)
- 21:04, 28 October 2008 (diff | hist) N Team:Waterloo/Notebook/DNA Extraction (Team:Waterloo/Notebook/DNA Extraction moved to Team:Waterloo/Notebook/Protocols/DNA Extraction) (top)
- 21:04, 28 October 2008 (diff | hist) m Team:Waterloo/Notebook/Protocols/DNA Extraction (Team:Waterloo/Notebook/DNA Extraction moved to Team:Waterloo/Notebook/Protocols/DNA Extraction)
- 21:02, 28 October 2008 (diff | hist) N Team:Waterloo/Notebook/DNA Digestion (Team:Waterloo/Notebook/DNA Digestion moved to Team:Waterloo/Notebook/Protocols/DNA Digestion) (top)
- 21:02, 28 October 2008 (diff | hist) m Team:Waterloo/Notebook/Protocols/DNA Digestion (Team:Waterloo/Notebook/DNA Digestion moved to Team:Waterloo/Notebook/Protocols/DNA Digestion)
- 21:02, 28 October 2008 (diff | hist) N Team:Waterloo/Notebook/Colony PCR (Team:Waterloo/Notebook/Colony PCR moved to Team:Waterloo/Notebook/Protocols/Colony PCR) (top)
- 21:02, 28 October 2008 (diff | hist) m Team:Waterloo/Notebook/Protocols/Colony PCR (Team:Waterloo/Notebook/Colony PCR moved to Team:Waterloo/Notebook/Protocols/Colony PCR)
- 21:01, 28 October 2008 (diff | hist) Team:Waterloo/Notebook/Protocols (top)
- 21:00, 28 October 2008 (diff | hist) N Team:Waterloo/Notebook/Miniprep (Team:Waterloo/Notebook/Miniprep moved to Team:Waterloo/Notebook/Protocols/Miniprep) (top)
- 21:00, 28 October 2008 (diff | hist) m Team:Waterloo/Notebook/Protocols/Miniprep (Team:Waterloo/Notebook/Miniprep moved to Team:Waterloo/Notebook/Protocols/Miniprep)
- 20:59, 28 October 2008 (diff | hist) Team:Waterloo/Sponsors/Protocols (Removing all content from page) (top)
- 20:58, 28 October 2008 (diff | hist) N Team:Waterloo/Notebook/Protocols (New page: {| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center" !align="center"|Home !align="cent...)
- 20:58, 28 October 2008 (diff | hist) Team:Waterloo/Notebook
- 20:57, 28 October 2008 (diff | hist) N Team:Waterloo/Sponsors/Protocols (New page: {| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center" !align="center"|Home !align="cent...)
- 20:56, 28 October 2008 (diff | hist) Team:Waterloo/Notebook
- 20:23, 28 October 2008 (diff | hist) N File:Fermentas.PNG (top)
- 20:22, 28 October 2008 (diff | hist) N File:DiaMed.PNG (top)
- 20:22, 28 October 2008 (diff | hist) N File:BioRad.PNG (top)
- 20:21, 28 October 2008 (diff | hist) Team:Waterloo/Sponsors
- 20:15, 28 October 2008 (diff | hist) N File:SNCLavalin.PNG (top)
- 20:14, 28 October 2008 (diff | hist) N File:EngFac.PNG (top)
- 20:13, 28 October 2008 (diff | hist) Team:Waterloo/Sponsors
- 20:11, 28 October 2008 (diff | hist) N File:ChemEng.PNG (top)
- 20:10, 28 October 2008 (diff | hist) N File:NEB.PNG (top)
- 20:09, 28 October 2008 (diff | hist) N File:FSF.PNG (top)
- 20:08, 28 October 2008 (diff | hist) N File:DeptBio.PNG (top)
- 20:08, 28 October 2008 (diff | hist) Team:Waterloo/Sponsors
- 20:04, 28 October 2008 (diff | hist) Team:Waterloo/Sponsors
- 20:03, 28 October 2008 (diff | hist) N File:SciFac.PNG (top)
- 20:03, 28 October 2008 (diff | hist) Team:Waterloo/Sponsors
- 19:58, 28 October 2008 (diff | hist) Team:Waterloo/Sponsors
- 19:46, 28 October 2008 (diff | hist) Team:Waterloo/Sponsors
- 19:43, 28 October 2008 (diff | hist) N File:MEF.PNG (top)
- 19:18, 28 October 2008 (diff | hist) Team:Waterloo/Sponsors
- 19:08, 28 October 2008 (diff | hist) Team:Waterloo/Team
- 19:07, 28 October 2008 (diff | hist) m Team:Waterloo/Team
- 18:57, 28 October 2008 (diff | hist) Team:Waterloo/Team
- 18:30, 28 October 2008 (diff | hist) Team:Waterloo/Team
- 18:07, 28 October 2008 (diff | hist) N File:Adam Stinchcombe.PNG (top)
- 18:06, 28 October 2008 (diff | hist) Team:Waterloo/Team
- 18:03, 28 October 2008 (diff | hist) Team:Waterloo/Team
- 18:00, 28 October 2008 (diff | hist) N File:Vanja Polic.PNG (top)
- 18:00, 28 October 2008 (diff | hist) Team:Waterloo/Team
- 17:53, 28 October 2008 (diff | hist) File:Emily Wedland.PNG (uploaded a new version of "Image:Emily Wedland.PNG": Reverted to version as of 17:18, 28 October 2008) (top)
- 17:49, 28 October 2008 (diff | hist) File:Emily Wedland.PNG (uploaded a new version of "Image:Emily Wedland.PNG")
- 17:48, 28 October 2008 (diff | hist) Team:Waterloo/Team
- 17:18, 28 October 2008 (diff | hist) N File:Emily Wedland.PNG
- 17:17, 28 October 2008 (diff | hist) Team:Waterloo/Team
- 14:53, 28 October 2008 (diff | hist) Team:Waterloo/Sponsors
- 14:51, 28 October 2008 (diff | hist) Team:Waterloo/Sponsors
- 14:47, 28 October 2008 (diff | hist) Team:Waterloo/Sponsors
- 14:08, 28 October 2008 (diff | hist) Team:Waterloo/Sponsors
- 14:05, 28 October 2008 (diff | hist) Team:Waterloo/Sponsors
- 13:53, 28 October 2008 (diff | hist) Team:Waterloo/Sponsors
- 13:31, 28 October 2008 (diff | hist) Team:Waterloo/Sponsors
- 13:09, 28 October 2008 (diff | hist) Team:Waterloo/Sponsors
- 19:35, 27 October 2008 (diff | hist) N Team:Waterloo/Notebook/Making SOB Media (New page: <big><b><u>Making SOB Medium</u></b></big><br> Used in growing bacteria for preparing chemically competent cells <br> <b>Ingredients </b><br> - 0.5% (w/v) yeast extract <br> - 2% (w/v)...)
- 19:28, 27 October 2008 (diff | hist) N Team:Waterloo/Notebook/Protocols/Competent Cells (New page: <big><b><u>Recipe for Competent Cells </u></b></big> <br> <b>Materials </b><br> Frozen stock DH5a <br> 250mL Erlenmeyer Flask (w/ caps) <br> LB tubes <br> Sterile LB <br> <b>Instructio...)
- 19:08, 27 October 2008 (diff | hist) N Team:Waterloo/Notebook/Making LB Media (New page: <big><b><u>Making LB Media</u></b></big> <br> <b>Materials </b><br> 500 mL media bottle or rack of test tubes <br> 1 L beaker <br> Magnetic stir bar <br> Tryptone <br> Yeast extract<br> ...)
- 18:59, 27 October 2008 (diff | hist) N Team:Waterloo/Notebook/Making AI Media (New page: <b><u><big>Making Auto Induction (AI) Media</big></u></b><br> <b>Before you start </b><br> Certain parts are on variable copy number plasmids. The plasmid is normally low copy number, b...)
- 18:47, 27 October 2008 (diff | hist) N Team:Waterloo/Notebook/Protocols/Transformation (New page: <b><u><big>Transformation</big></u></b><br> <b>Before you start </b><br> Cells lose their competence once thawed, so be sure to:<br> a) keep them on ice at all times.<br> b) use cells imm...)
- 18:26, 27 October 2008 (diff | hist) N Team:Waterloo/Notebook/Protocols/Inoculation (New page: <big><b><u>Inoculation Protocol </u></b></big><br> <b>Materials </b><br> DNA Samples <br> Ampicillin (2µL/sample)<br> Kanamycin (2.5µL/sample)<br> Liquid LB Media (1 tube/sample)<br> <...)
- 18:20, 27 October 2008 (diff | hist) N Team:Waterloo/Notebook/Protocols/Glycerol Stock Preparation (New page: <b><u><big>Glycerol Stock Preparation </big></u></b><br> <b>Before you start </b><br> - It is important to use aseptic technique when carrying out the procedures <br> <b>Materials </b><...)
- 18:10, 27 October 2008 (diff | hist) N Team:Waterloo/Notebook/Protocols/Ligation (New page: <big><b><u>Ligation (10µL recipe with 5 insert : 1 vector ratio)</u></b></big> <br> <b>Materials </b><br> MQ water <br> Ligase buffer<br> Ligase <br> Eppendorf tube<br> Vector DNA<br> I...)
- 18:03, 27 October 2008 (diff | hist) N Team:Waterloo/Notebook/Protocols/Gel Electrophoresis (New page: <b><u><big>Gel Electrophoresis</big></u></b><br> <b>Materials </b><br> Agarose <br> 1X TAE buffer <br> Gel Red<br> 1:10 diluted 1kb DNA ladder<br> Loading Dye<br> Gel Rig<br> <b>Instru...)
- 17:43, 27 October 2008 (diff | hist) N Team:Waterloo/Notebook/Protocols/DNA Extraction (New page: <b><u><big>DNA Extraction </big></u></b><br> <b>Materials </b><br> All required materials provided in the Fermentas DNA Extraction Kit (K0513)<br> <b>Instructions </b><br> 1. Follow the ...)
- 17:31, 27 October 2008 (diff | hist) Team:Waterloo/Notebook (→Protocols)
- 17:31, 27 October 2008 (diff | hist) N Team:Waterloo/Notebook/Protocols/DNA Digestion (New page: <b><u><big>Digestion of Plasmid DNA </big></u></b><br> <b>Before you start </b><br> - Know what is the total digestion volume mix. From that you can determine the enzyme concentration whi...)
- 17:25, 27 October 2008 (diff | hist) N Team:Waterloo/Notebook/Diagnostic Digestion (New page: <b><u><big>Diagnostic Digestion of Plasmid DNA </big></u></b><br> <b>Before you start </b><br> - Know what is the total digestion volume mix. From that you can determine the enzyme concen...) (top)
- 17:02, 27 October 2008 (diff | hist) Team:Waterloo/Notebook/Protocols/Colony PCR
- 17:00, 27 October 2008 (diff | hist) Team:Waterloo/Notebook/Protocols/Colony PCR
- 16:57, 27 October 2008 (diff | hist) N Team:Waterloo/Notebook/Protocols/Colony PCR (New page: <b><u><big>Colony PCR </big></u></b><br> <b>Materials</b><br> - 19 µL of Master Mix<br> - 1 µL<br> <u>Calculations for Master Mix</u><br> ___ number of colonies (and control) <br> (__...)
- 15:44, 27 October 2008 (diff | hist) N Team:Waterloo/Notebook/Protocols/Miniprep (New page: <b><u><big>1-2-3 Miniprep (Resuspension, Lysis, Neutralization)</big></u></b><br> <b>Before you start</b><br> Prepare solutions 1, 2, and 3. Only solution 2 needs to be made fresh. Soluti...)
- 18:26, 26 October 2008 (diff | hist) N File:1Sequence (6).jpg (top)
- 18:17, 26 October 2008 (diff | hist) N File:1Sequence (4).jpg (top)
- 18:16, 26 October 2008 (diff | hist) N File:1Sequence (5).jpg (top)
- 18:15, 26 October 2008 (diff | hist) File:Sequence (5).jpg (uploaded a new version of "Image:Sequence (5).jpg") (top)
- 18:15, 26 October 2008 (diff | hist) Team:Waterloo/Project (→Steps in Assembly)
- 18:12, 26 October 2008 (diff | hist) Team:Waterloo/Project (→Steps in Assembly)
- 18:11, 26 October 2008 (diff | hist) Team:Waterloo/Project (→Steps in Assembly)
- 18:11, 26 October 2008 (diff | hist) File:Sequence (5).jpg (uploaded a new version of "Image:Sequence (5).jpg")
- 18:05, 26 October 2008 (diff | hist) Team:Waterloo/Project (→Steps in Assembly)
- 18:04, 26 October 2008 (diff | hist) File:Sequence (4).jpg (uploaded a new version of "Image:Sequence (4).jpg") (top)
- 18:04, 26 October 2008 (diff | hist) Team:Waterloo/Project (→Steps in Assembly)
- 18:03, 26 October 2008 (diff | hist) File:Sequence (4).jpg (uploaded a new version of "Image:Sequence (4).jpg": Reverted to version as of 01:32, 25 October 2008)
- 18:03, 26 October 2008 (diff | hist) File:Sequence (4).jpg (uploaded a new version of "Image:Sequence (4).jpg")
- 17:58, 26 October 2008 (diff | hist) File:Sequence (3).jpg (uploaded a new version of "Image:Sequence (3).jpg") (top)
- 17:54, 26 October 2008 (diff | hist) Team:Waterloo/Project (→Steps in Assembly)
- 17:53, 26 October 2008 (diff | hist) File:Sequence (2).jpg (uploaded a new version of "Image:Sequence (2).jpg") (top)
- 17:53, 26 October 2008 (diff | hist) File:Sequence (2).jpg (uploaded a new version of "Image:Sequence (2).jpg")
- 17:46, 26 October 2008 (diff | hist) Team:Waterloo/Project (→Steps in Assembly)
- 17:44, 26 October 2008 (diff | hist) File:Sequence (1).jpg (uploaded a new version of "Image:Sequence (1).jpg") (top)
- 03:24, 26 October 2008 (diff | hist) N File:Construct 1.jpg (top)
- 03:17, 26 October 2008 (diff | hist) N File:Construct 1.JPG (top)
- 16:05, 24 October 2008 (diff | hist) N Waterloo/23 October 2008 (New page: <u>08/10/23 Thu</u><br> [x] Kit prep rfp'2/5 pRecA3<br> [x] Nanodrop<br> [x] Send above samples for sequencing along with rTT+POCI and rPlac+TT samples from September 17th<br> [x] make m...) (top)
- 16:01, 24 October 2008 (diff | hist) N Waterloo/21 October 2008 (New page: <u>08/10/21 Tues</u><br> [x] run gel of all colony PCR results<br> [x] inoculate decent looking stuff<br> [x] bring official wiki up to date with lab activities<br>) (top)
- 15:58, 24 October 2008 (diff | hist) N Waterloo/22 October 2008 (New page: <u>08/10/22 Wed</u><br> [x] kit prep of decent looking samples from 10/21 gel<br> - inoculations for all 6 tubes of rfp'2/5 PrecA3 failed<br> [x] nanodrop kit preps<br> [x] send for seque...) (top)
- 15:53, 24 October 2008 (diff | hist) Team:Waterloo/Notebook (→Protocols)
- 15:52, 24 October 2008 (diff | hist) Team:Waterloo/Notebook (→Recipes)
- 15:50, 24 October 2008 (diff | hist) Team:Waterloo/Notebook (→Recipes)
- 15:50, 24 October 2008 (diff | hist) Team:Waterloo/Notebook (→Protocols)
- 15:28, 24 October 2008 (diff | hist) Team:Waterloo/Notebook
- 15:09, 24 October 2008 (diff | hist) Team:Waterloo/Sponsors
- 14:59, 24 October 2008 (diff | hist) Team:Waterloo/Notebook
- 14:57, 24 October 2008 (diff | hist) Team:Waterloo/Sponsors
- 14:56, 24 October 2008 (diff | hist) Team:Waterloo/Modeling
- 14:55, 24 October 2008 (diff | hist) Team:Waterloo/Parts
- 14:54, 24 October 2008 (diff | hist) Team:Waterloo/Project
- 14:53, 24 October 2008 (diff | hist) Team:Waterloo/Team
- 14:53, 24 October 2008 (diff | hist) Team:Waterloo/Notebook
- 14:36, 24 October 2008 (diff | hist) N Team:Waterloo/Sponsors (New page: ==2008 Sponsors== ===Gold Level=== <html><center><img src="http://igem.uwaterloo.ca/share/Logos/MEFLogo.png" width="453px" height="147px"><br> <a href="http://www.mef.uwaterloo.ca/"> Mathe...)
- 14:33, 24 October 2008 (diff | hist) Team:Waterloo
- 15:45, 21 October 2008 (diff | hist) N Waterloo/20 October 2008 (New page: <u>08/10/20 Monday</u><br> [x] patches of transformants<br> [x] Stuff tips and general housekeeping<br> [x] Autoclave<br> [x] colony PCR of patches from morning<br>) (top)
- 15:41, 21 October 2008 (diff | hist) N Waterloo/19 October 2008 (New page: <u>08/10/19 Sunday</u><br> [x] transformation of ligations<br>) (top)
- 15:37, 21 October 2008 (diff | hist) N Waterloo/18 October 2008 (New page: <u>08/10/18 Saturday</u><br> [] kit prep Plac-rfp'<br> - Inoculations seemed to have failed again<br> [x] digest (digest 16 ul of a ~600-800 bp part in pSB1A2 so we can subclone into this...) (top)
- 15:33, 21 October 2008 (diff | hist) N Waterloo/17 October 2008 (New page: <u>08/10/17 Friday</u><br> [x] kit prep of transformant inoculants<br> - also, kit prep of plac-rfp'<br> - Inoculations for Plac-rfp' from frozen stock did not work<br> [] digest (digest...) (top)
- 15:27, 21 October 2008 (diff | hist) N Waterloo/16 October 2008 (New page: <u>08/10/16 Thursday</u><br> [x] inoculate transformants (use one colony from each, but inoculate in duplicate!)<br> [x] BIOMORTAR<br> [x] update Box Contents Google Doc with location nu...) (top)
- 15:25, 21 October 2008 (diff | hist) N Waterloo/15 October 2008 (New page: <u>08/10/15 Wednesday</u><br> [x] transform c0051' 6, c0051''-4, PMEI gene 6 (1,2,4)<br>) (top)
- 15:22, 21 October 2008 (diff | hist) N Waterloo/14 October 2008 (New page: <u>08/10/14 Tuesday</u> <br> [] send stuff for sequencing<br> [] inoculate stuff to kit prep more DNA of (running low on some of the stuff we need to subclone/assemble)<br> - c0051', c00...) (top)
- 15:17, 21 October 2008 (diff | hist) N Waterloo/13 October 2008 (New page: <u>08/10/13 Monday</u><br> [] patch of transformants<br> [] colony PCR in evening<br> [] prepare stuff to send or resend for sequencing - a few things are now listed on the sequencing f...) (top)
- 15:15, 21 October 2008 (diff | hist) N Waterloo/12 October 2008 (New page: <u>08/10/12 Sunday</u><br> [] ligation either into<br> a) pJET <br> b) BioBrick vector subcloning<br> [] transformation of ligations<br> [] during next transformation, include: <br> a) ...) (top)
- 15:10, 21 October 2008 (diff | hist) N Waterloo/11 October 2008 (New page: <u>08/10/11 Saturday</u><br> [] kit prep<br> [] digest<br> [] gel for GEx<br> [] make glycerol stocks<br> [] GEx<br>) (top)
- 15:09, 21 October 2008 (diff | hist) N Waterloo/10 October 2008 (New page: <u>08/10/10 Friday </u><br> [x] fill more tubes with LB media<br> [] inoculate stuff to kit prep more DNA of (running low on some of the stuff we need to subclone/assemble)<br> [] inocu...) (top)
- 15:05, 21 October 2008 (diff | hist) N Waterloo/9 October 2008 (New page: <u>08/10/09 Thursday</u><br> [x] kit prep of cultures inoculated on Wed<br> [x] digest<br> a) above kit preps<br> b) rfp"-2 and -5 (X/P)<br> c) anything from the sequencing results that ...) (top)
- 13:44, 21 October 2008 (diff | hist) N Waterloo/8 October 2008 (New page: <u>08/10/08 Wednesday</u> <br> [x] kit prep POcI<br> [x] digest stuff for cloning (pJET(rfp") - E/S and S/P; pSB2K3(PRecA) - S/P; pSB1A2(POcI) - S/P)<br> [x] gel for GEx:<br> a) digests<...) (top)
- 13:36, 21 October 2008 (diff | hist) N Waterloo/7 October 2008 (New page: <u>08/10/07 Tuesday</u> <br> [x] take picture of Gel from yesterday<br> [x] GEx band from yesterday<br> [] ligation of PrecA into biobrick vector<br> [] transformation<br> [x] Inoculated ...) (top)
- 13:33, 21 October 2008 (diff | hist) N Waterloo/6 October 2008 (New page: <u>08/10/06 Monday</u> <br> [x] prepare and send stuff for sequencing<br> [x] Digest vector for cloning in PrecA<br> [] GEx vector<br>) (top)
- 13:29, 21 October 2008 (diff | hist) N Waterloo/5 October 2008 (New page: <u>08/10/05 Sunday</u><br> [x] kit prep cultures from Saturday<br>) (top)
- 13:28, 21 October 2008 (diff | hist) N Waterloo/4 October 2008 (New page: <u>08/10/04 Saturday</u><br> [x] check gel of colony PCR<br> [x] Inoculate positive colonies<br>) (top)
- 13:27, 21 October 2008 (diff | hist) N Waterloo/3 October 2008 (New page: <u>08/10/03 Friday</u> <br> [x] Patch transformants <br> [x] set up colony PCR<br> [] Make gel for tomorrow) (top)
- 13:23, 21 October 2008 (diff | hist) N Waterloo/2 October 2008 (New page: <u>08/10/02 Thursday</u> <br> [] Patch again<br> - Transformation didn't work<br> [x] pour Km and Ap plates<br> [x] digest and run gel<br> [x] Gel extraction of digest<br> [x] ligation<br...) (top)
- 19:47, 20 October 2008 (diff | hist) N Waterloo/18 September 2008 (New page: <u>08/09/18 Thursday </u><br> [x] continue with crossover PCR<br> - run with KOD at various DMSO%<br> - RFP' in the G box should be used as positive control using VR and Pre+RBS primers a...) (top)
- 19:44, 20 October 2008 (diff | hist) N Waterloo/17 September 2008 (New page: <u>080917 Wed</u><br> [x] rerun diagnostic digest and gel on potential BioBrick subclones using Buffer O, since it's just a NotI single digest<br> [x] make lots of media! (fill 5 mL tube...) (top)
- 19:41, 20 October 2008 (diff | hist) N Waterloo/16 September 2008 (New page: <u>080916 Tue</u> <br> [x] run gel of PCR products<br> [x] based on above results, set up PCR of best conditions using Charles lab KOD Hot Start<br> [] make lots of liquid media! (fill...) (top)
- 19:39, 20 October 2008 (diff | hist) Waterloo/12 September 2008 (top)
- 19:38, 20 October 2008 (diff | hist) N Waterloo/15 September 2008 (New page: <u>080915 Mon</u><br> [x] rerun diagnostic digest and gel on potential BioBrick subclones<br> - seems to have contamination again<br> [x] run PCR of crossover stuff using Taq master mix ...) (top)
- 19:36, 20 October 2008 (diff | hist) N Waterloo/12 September 2008 (New page: <u>08/09/12 Friday</u><br> [] based on gel results, get stuff ready to send for sequencing<br> - gel results looked terrible therefore no sequencing for Friday<br> [] send promising Thurs...)
- 19:33, 20 October 2008 (diff | hist) N Waterloo/11 September 2008 (New page: <u>080911 Thu</u><br> [x] kit prep inoculated cultures from Wednesday, Sept. 10<br> [x] diagnostic digest of above<br> [x] diagnostic gel of above<br>) (top)
- 19:32, 20 October 2008 (diff | hist) N Waterloo/10 September 2008 (New page: <u>080910 Wed</u> <br> [x] order crossover PCR primers<br> [x] make CaCl2 competent E. coli of strain TG1 which contains lacI for Plac testing<br> - Box E, 10 500uL aliquots<br> [x] anal...) (top)
- 19:24, 20 October 2008 (diff | hist) Waterloo/9 September 2008 (top)
- 19:21, 20 October 2008 (diff | hist) N Waterloo/8 September 2008 (New page: <u>080908 Mon</u><br> [x] check Sunday's transformation results<br> Putative good constructs yet to be sequenced:<br> [x] inoculate culture from transformants of parts for sequencing (an...) (top)
- 19:21, 20 October 2008 (diff | hist) Waterloo/9 September 2008
- 19:18, 20 October 2008 (diff | hist) N Waterloo/9 September 2008 (New page: <u>080908 Mon</u><br> [x] check Sunday's transformation results<br> Putative good constructs yet to be sequenced:<br> [x] inoculate culture from transformants of parts for sequencing (an...)
- 19:10, 20 October 2008 (diff | hist) N Waterloo/7 September 2008 (New page: <u>080907 Sun</u> <br> [x] redo transformation:<br> a) parts to sequence<br> b) synthesized parts (Ap)<br> [x] inoculate cultures from:<br> a) Saturday's transformants (only gene6 and Pm...) (top)
- 19:06, 20 October 2008 (diff | hist) N Waterloo/6 September 2008 (New page: <u>080906 Sat</u> <br> [x] compile list of things to sequence this week<br> [x] run check gel of GEx products<br> - everything looked good (had ~correctly sized bands for each GEx)<br> [x...) (top)
- 18:55, 20 October 2008 (diff | hist) N Waterloo/5 September 2008 (New page: <u>080905 Fri </u> <br> [x] analyze Thursday's gel; select which parts to move forward with<br> [x] redo diag digest and gel of gene6 vs. putative gene6'<br> - gene6' looks too much larg...) (top)
- 18:49, 20 October 2008 (diff | hist) N Waterloo/4 September 2008 (New page: <u>080904 Thu </u> <br> [x] redo diag of things that didn't work<br> [x] fill tips, tubes, etc.<br> [x] annealed fwd and rev oligos for pRecA<br>) (top)
- 18:48, 20 October 2008 (diff | hist) N Waterloo/3 September 2008 (New page: <u>080903 Wed</u> <br> [x] run NotI diagnostic gel:<br> - pJET(gene6) vs. pSB1A2(RBS-gene6?) --> [if looks okay, send for sequencing]<br> - cI'? (both C0051'? and C0052'?) (in good box) ...) (top)
- 18:46, 20 October 2008 (diff | hist) Waterloo/28 August 2008 (top)
- 18:44, 20 October 2008 (diff | hist) N Waterloo/28 August 2008 (New page: <u>08/08/28 Thurs</u> <br> - Last day for coops<br> [] miniprep of c0051'', c0052'' <br> [] diag digest<br> [] diag gel<br>)
- 18:42, 20 October 2008 (diff | hist) N Waterloo/27 August 2008 (New page: <u>08/08/27 Wed</u> <br> - Bioseminar presentation<br> [] frozen stock of transformants<br> [] p/s/i of transformation (c0051'', c0052'')<br>) (top)
- 18:41, 20 October 2008 (diff | hist) N Waterloo/26 August 2008 (New page: <u>08/08/26 Tues</u> <br> [] competent cells<br> [] gel of full digestion (c0051', c0052', TT, TT, RBS, RBS)<br> [] gel extraction<br> [] ligation<br> [] transformation<br> [] inoculate...) (top)
- 18:34, 20 October 2008 (diff | hist) N Waterloo/25 August 2008 (New page: <u>08/08/25 Mon</u><br> [x] frozen stock of pJET + gene 6 (27 and 29)<br> [x] diagnostic digest of gene6' results - biobrick double digest (do not have the appropriate enzyme in stock to ...) (top)
- 18:30, 20 October 2008 (diff | hist) N Waterloo/24 August 2008 (New page: <u>08/08/24 Sun</u> <br> [x] reinoculate pJET + gene 6<br> [] inoculate 2 tubes each of a couple RBS-gene6 samples that looked good based on Friday's colony PCR results<br> - Monday: make...) (top)
- 18:23, 20 October 2008 (diff | hist) N Waterloo/22 August 2008 (New page: <u>08/08/22 Fri</u> <br> [x] miniprep of TT, RBS, 8 samples<br> [] prepare list for transformations<br>) (top)
- 18:21, 20 October 2008 (diff | hist) N Waterloo/21 August 2008 (New page: <u>08/08/21 Thurs</u> <br> [x] gel of full digestion digestion<br> - not left long enough, will repeat<br> - TT did not work, will reinoculate<br> [] gel extraction<br> [] ligation<br> []...) (top)
- 18:07, 20 October 2008 (diff | hist) N Waterloo/20 August 2008 (New page: <u>08/08/20 Wed </u> <br> [x] miniprep of inoculatants<br> [x] diagnostic digestion<br> [x] diag gel of inoculants and PCR from Tues<br> [] diagnostic gel of sketchy DNA<br> [] gel and ...) (top)
- 17:59, 20 October 2008 (diff | hist) N Waterloo/19 August 2008 (New page: <u>08/08/19 Tues</u> <br> [x] inoculate good colonies from colony PCR<br> [x] glycerol stock of iGEM stabs<br> [x] repeat PCR phb testing<br> - Try the ones that didn't work again, thi...) (top)
- 17:56, 20 October 2008 (diff | hist) N Waterloo/18 August 2008 (New page: <u>08/08/18 Mon</u> <br> [x] check gel of PHB PCR products<br> [x] GEx of PHB PCR products (if PCR successful based on check gel)<br> [x] colony PCR of c0051', c0052', gene 6'<br> [x] di...) (top)
- 17:52, 20 October 2008 (diff | hist) N Waterloo/17 August 2008 (New page: <u>08/08/17 Sun</u> <br> [x] PCR of PHB A, B, C genes using genomic DNA and Phusion polymerase<br> - Try it with the 1:100 dilution of Rm1021 CB (cosmid bank) DNA, left in the "good const...) (top)
- 17:40, 20 October 2008 (diff | hist) N Waterloo/15 August 2008 (New page: <u>08/08/15 Fri</u> <br> [x] diag gel of PCR from yesterday<br> [x] PCR (colony PCR testing with RBS)<br> [x] diag gel of PCR<br>) (top)
- 17:37, 20 October 2008 (diff | hist) N Waterloo/14 August 2008 (New page: <u>8/08/14 Thurs</u> <br> [x] send gene 6 for sequencing<br> [] p/s/i of c0052', c0051', T7 gene 6 + RBS<br> [x] miniprep of inoculatants from wed<br> [x] Gel of overnight PCR of phbA ...) (top)
- 17:28, 20 October 2008 (diff | hist) N Waterloo/13 August 2008 (New page: <u>08/08/13 Wed </u><br> [x] gel of full digestion (c0052', c0051', T7 gene6-27)<br> [x] gel extraction<br> [x] check gel of extraction<br> [x] ligation of parts from extraction and c0052...) (top)
- 17:16, 20 October 2008 (diff | hist) N Waterloo/12 August 2008 (New page: <u>08/08/12 Tues </u><br> [x] kit prep T7 gene 6 and gene 5 - High Priority<br> [x] diagnostic digest of kit prep of gene 6&5<br> - Double (e/s) and Single (e)<br> [] run gel of diagnost...) (top)
- 17:12, 20 October 2008 (diff | hist) N Waterloo/11 August 2008 (New page: <u>08/08/11 Mon</u> <br> [x] transformation of T7 gene 5 (4) in pJET<br> [x] Inoculation of gene 5&6 colonies from pJET transformants<br> [x] inoculate c0051' and c0052'<br> [x] inoculate ...) (top)
- 17:09, 20 October 2008 (diff | hist) N Waterloo/10 August 2008 (New page: <u>10/08/08 Sun </u> <br> [] Inoculation of gene 5&6 colonies from pJET transformants<br> - no one was able to come in for this) (top)
- 17:06, 20 October 2008 (diff | hist) N Waterloo/9 August 2008 (New page: <u>09/08/08 Sat</u> <br> [x] gel of PCR T7 gene 5 from friday<br> [x] gel extraction of PCR<br> [x] pJET ligation<br> [x] transformation<br>) (top)
- 20:28, 17 October 2008 (diff | hist) N Waterloo/7 August 2008 (New page: <u>08/08/07 Thurs</u> <br> [] p/s/i of c0051' and c0052' - will wait until colonies are bigger<br> [x] repeat of test PCR T7 gene 5 <br> [x] diagnostic gel of PCR<br> [] if colonies are b...) (top)
- 20:26, 17 October 2008 (diff | hist) N Waterloo/8 August 2008 (New page: <u>08/08/08 Fri</u> <br> [x] colony PCR of T7 gene 6, c0051', c0052' <br> [x] repeat of PCR t7 gene 5 <br> [x] diag gel of colony pcr and t7 gene 5 PCR <br>) (top)
- 18:41, 17 October 2008 (diff | hist) N Waterloo/6 August 2008 (New page: <u>08/08/06 Wed </u> <br> [x] extraction gel of PCR (gene 5) and full digest (TT, RBS, ligation controls)<br> [x] gel extraction of above<br> [x] ligation of RBS + C0051, RBS + C0052, lig...) (top)
- 18:27, 17 October 2008 (diff | hist) N Waterloo/5 August 2008 (New page: <u>08/08/05 Tues</u> <br> [x] full gel of PCR T7 gene 6 (samples 3 and 4)<br> [x] gel extract gene 6. (x2)<br> [x] diagnostic gel of PCR gene 6 (x2)<br> [x] full digestion of RBS (1) TT ...) (top)
- 18:56, 16 October 2008 (diff | hist) N Waterloo/1 August 2008 (New page: <u>08/08/01 Fri</u> <br> [x] Repeat gene 6 PCR with iProof<br> - iProof worked, but not the home-made PFU<br> - positive control didn't work either<br> [x] diagnostic gel<br>) (top)
- 18:54, 16 October 2008 (diff | hist) N Waterloo/31 July 2008 (New page: <u>08/07/31 Thurs</u><br> [x] full gel of PCR products (gene 6)<br> [x] diagnostic gel of t7 gene 6, t7 gene 5 (speedvacced from yesterday)<br> - Gene 5 is ok, but Gene 6 didn't work<br>...) (top)
- 18:50, 16 October 2008 (diff | hist) N Waterloo/30 July 2008 (New page: <u>08/07/30 Wed</u> <br> [] p/s/i of POCI rfp and gfp<br> - no growth observed<br> - religate and retransform<br> [x] full digestion of c0052, c0051<br> [x] diagnostic digest of yesterd...) (top)
- 18:45, 16 October 2008 (diff | hist) N Waterloo/29 July 2008 (New page: <u>08/07/29 Tues</u><br> [x] run full gel of new PCR results<br> [x] full gel of PCR 12 (25/7)<br> [x] transformation of POCI, gfp and rfp<br> [x] gel extraction<br> [x] frozen stock ...) (top)
- 18:43, 16 October 2008 (diff | hist) N Waterloo/28 July 2008 (New page: <u>08/07/28 Mon</u><br> [x] gel of PCR<br> [x] run check gel of gel extraction product<br> - not successful. looks like no dna<br> - will repeat PCR<br> [] if above successful, Clone T7...) (top)
- 18:39, 16 October 2008 (diff | hist) N Waterloo/25 July 2008 (New page: <u>08/07/25 Fri</u><br> [x] autoclave waste<br> [x] PCR testing of different conditions (glycerol, DMSO, iProof, MgSO4)<br> [x] full gel of Gene 6<br> [x] gel extraction/purification o...) (top)
- 18:38, 16 October 2008 (diff | hist) N Waterloo/24 July 2008 (New page: <u>08/07/24 Thurs</u> <br> [x] diagnostic gel of PCR cloning T7 gene 5<br> [x] throw out sketchy DNA<br> [] if cloning worked, full gel and cloning into commercial vector<br> - Only pos...) (top)
- 18:36, 16 October 2008 (diff | hist) N Waterloo/23 July 2008 (New page: <u>8/07/23 Wed</u> <br> [x] decide on a reverse TT <br> [] check details of synthesis deal <br> [] get sequences, design, etc. to order<br> [x] miniprep transformants <br> - Some transfo...) (top)
- 18:31, 16 October 2008 (diff | hist) N Waterloo/22 July 2008 (New page: <u>08/07/22 Tues</u> <br> [x] autoclave tips/tubes<br> [x] cloning PCR of T7 genes 5 and 6, PmeIR (colony PCR) from LB broth<br> [x] diagnostic digest and gel of c0052'(2-8) and gene DN...) (top)
- 18:29, 16 October 2008 (diff | hist) N Waterloo/21 July 2008 (New page: <u>08/07/21 Mon</u> <br> [x] ligation of plac rfp'', POCI rfp'', POCI gfp'', Pconst rfp'', Pconst gfp''<br> [x] transformations of ligations (and plac gfp'' from previously ligated DNA)<...) (top)
- 18:26, 16 October 2008 (diff | hist) N Waterloo/18 July 2008 (New page: <u>08/07/18 Fri</u> <br> [x] miniprep c0052' transformant incoulations and glycerol stock inoculations<br> [x] gel extraction and purification of plac rfp'', plac gfp'', POCI rfp'', POCI...) (top)
- 18:24, 16 October 2008 (diff | hist) N Waterloo/17 July 2008 (New page: <u>08/07/17 Thurs</u> <br> [x] miniprep of transformants inoculations (rfp'')<br> [x] diagnostic digest of transformants and c0051, c0052<br> [x] gel of diagnostic digest<br> [x] glyce...) (top)
- 18:09, 16 October 2008 (diff | hist) N Waterloo/16 July 2008 (New page: <u>08/07/16 Wed</u> <br> [] colony PCR of CI' (51) and CI' (52)<br> [] inoculation<br> [] test Pfu with new buffer at the same time<br> - no growth in ligation plates of c0052 and c0051<b...) (top)
- 18:03, 16 October 2008 (diff | hist) N Waterloo/15 July 2008 (New page: <u>08/07/15 Tues</u> <br> [x] observe results from competent cells test, rfp'', C0052', C0051', streaked placgfp'' to UV competent cells<br> - placgfp'' grew, [rfp'', C0052',C0051' did no...) (top)
- 17:54, 16 October 2008 (diff | hist) N Waterloo/14 July 2008 (New page: <u>08/07/14 Mon</u> <br> [x] transformation of ligation (rfp''), CI' (51), CI' (52)<br> [x] competent cells test (LB and no DNA, LB and DNA, +ve and no DNA, +ve and DNA)<br> [x] make I...) (top)
- 17:52, 16 October 2008 (diff | hist) N Waterloo/11 July 2008 (New page: <u>8/07/11 Fri</u> <br> [x] gel of diagnostic digest from yesterday.<br> [x] miniprep P. mendocina, stabs from iGEM (2).<br> [x] Make glycerol stock of P. mendocina KR1, stabs if it gr...) (top)
- 17:49, 16 October 2008 (diff | hist) N Waterloo/10 July 2008 (New page: <u>08/07/10 Thurs</u> <br> [x] competent cells (finish)<br> [x] gel continued from yesterday (PCI (1-3), Pconst (1-2), PtetR (1-2), PLacOCI(1-3), TTr(2,5) and uncuts)<br> [x] gel purifi...) (top)
- 17:45, 16 October 2008 (diff | hist) N Waterloo/9 July 2008 (New page: <u>08/07/09 Wed</u> <br> [x] Diagnostic digest and gel of TTr (2 and 5, uncut and single cut) + Promoters (single cut + uncut + control)<br> [x] full digest of rfp' and tt and gel and ge...) (top)
- 17:42, 16 October 2008 (diff | hist) N Waterloo/8 July 2008 (New page: <u>08/07/08 Tues</u> <br> [x] autoclave SOC media<br> [x] Make more Amp plates. There are still 12 left for Kan<br> [x] make competent cells<br> - finished part I<br> [x] make glycero...) (top)
- 17:39, 16 October 2008 (diff | hist) N Waterloo/7 July 2008 (New page: <u>08/07/07 Mon</u> <br> [x] make SOC media and TE buffer and autoclave both<br> [x] elute PocI and lacI from 2008 registry, follow protocol exactly.<br> [x] transformation of 2008 regi...) (top)
- 17:35, 16 October 2008 (diff | hist) N Waterloo/4 July 2008 (New page: <u>08/07/04 Fri</u> <br> [] p/s/i of PocI and lacI if there is growth <br> - no growth observed, will elute from 2008 registry<br> [x] Mini Prep<br> - concentrations between 2700 and 40...) (top)
- 17:30, 16 October 2008 (diff | hist) N Waterloo/3 July 2008 (New page: <u>08/07/03 Thurs</u> <br> p/s/i<br> Transformation from (2/7), 3 samples <br> - TT(reverse) good, was put in 4 degree<br> - PocI and lacI had no growth, so retransformed<br> PCR to test...) (top)
- 16:06, 16 October 2008 (diff | hist) N Waterloo/2 July 2008 (New page: <u>08/07/02 Wed</u> <br> [x] Elution<br> - TT (reverse) B0014 1-1G<br> - PocI R0051<br> - lacI<br> [x] Transformation<br> - Controls<br> - above elutions (TT reverse, PocI, lacI)<br> [x...) (top)
- 15:58, 16 October 2008 (diff | hist) N Waterloo/27 June 2008 (New page: <u>08/06/27 Friday</u> <br> [x] Gel of 06/26 PCR products<br> - assess which pol.s/buffers are working will run PCR again<br> - GEx Pfu product (if successful) to sequence) (top)
- 15:54, 16 October 2008 (diff | hist) N Waterloo/26 June 2008 (New page: <u>08/06/26 Thursday</u> <br> *Should have all the registration parts in by today !!*<br> [x] Repeat Gel of 2007 Registry<br> [x] streak<br> - cc on LB<br> - Placgfp'' Transformation...) (top)
- 15:46, 16 October 2008 (diff | hist) N Waterloo/25 June 2008 (New page: <u>08/06/25 Wednesday</u><br> Mini prep, Partial digest and Diganostic gel<br> [x] 2007 registry parts (~ 1.5hr)<br> - Repeat Gel tomrrow<br> [] 2008 registry parts<br> - No growt...) (top)
- 15:40, 16 October 2008 (diff | hist) N Waterloo/24 June 2008 (New page: <u>08/06/24 Tuesday </u> <br> [x] streak/patch/innoculate 2007 and 2008 registry parts (13:00 - 14:00)<br> [x] re-transform one sample from yesterday and PocI (13:00)<br> - No growth...) (top)
- 15:33, 16 October 2008 (diff | hist) N Waterloo/23 June 2008 (New page: <u>08/06/23 Monday</u> <br> Morning <br> [x] Mini prep cultures from Sunday (2007 and 2008 registry) 10:00 - 12:30 <br> - 2007 done, but need to redo 2008 miniprep on Wednesda...) (top)
- 15:25, 16 October 2008 (diff | hist) N Waterloo/22 June 2008 (New page: <u>08/06/22 Sunday</u> <br> [x] Inoculated 07 registry parts we need and subcultured cultures from Thursday's transformations (they're all mixed together in the 37, but the transformation...) (top)
- 15:23, 16 October 2008 (diff | hist) N Waterloo/21 June 2008 (New page: <u>08/06/21 Saturday</u> <br> [x] Moved patch/streak plates to 4 degree (they're perched precariously in the igem box) <br> innoculate using cultures from streaked plate (?) and also in...) (top)
- 15:17, 16 October 2008 (diff | hist) N Waterloo/20 June 2008 (New page: <u>08/06/20 Friday</u> <br> To do: <br> [x] Make competent cells<br> [x] Mini prep PluxR-gfp"<br> [x] Diagnostic digest of mini preps (and run on gel) - 1 + 1.5 hr<br> - I think all tr...) (top)
- 15:10, 16 October 2008 (diff | hist) N Waterloo/19 June 2008 (New page: <u>08/06/19 Thursday</u><br> To do:<br> [x] Elution of registry parts ( including RecA promoter) - ~4 hrs<br> [x] Transformation (Plac gfp'' (?) and RecA promoter) - overlap...) (top)