Team:Paris/July 26

From 2008.igem.org

(Difference between revisions)
(New page: {{Paris/Calendar_Links|July 25|July 27}} == MiniPreps == *The protocol of MiniPrep of Promega has been done on all the clones cultivated on the 25th. {| border="1" |align="center"|'''...)
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|align="center"|MP100
|align="center"|MP100
-
|align="center"|
+
|align="center"|[http://partsregistry.org/Part:BBa_B0034 B0034]
-
|align="center"|
+
|align="center"|Strongest RBS (Efficiency = 1)
|-
|-
|align="center"|MP101
|align="center"|MP101
-
|align="center"|
+
|align="center"|[http://partsregistry.org/Part:BBa_J23101 J23101]
-
|align="center"|
+
|align="center"|Strongest Constitutive promoter
|-
|-
|align="center"|MP102
|align="center"|MP102
-
|align="center"|
+
|align="center"|[http://partsregistry.org/Part:BBa_J23109 J23109]
-
|align="center"|
+
|align="center"|Weak Constitutive promoter
|-
|-
|align="center"|MP103
|align="center"|MP103
-
|align="center"|
+
|align="center"|[http://partsregistry.org/Part:BBa_R0079 R0079]
-
|align="center"|
+
|align="center"|promoter pLas (lasr & PAI regulated)
|-
|-
|align="center"|MP104
|align="center"|MP104
-
|align="center"|
+
|align="center"|[http://partsregistry.org/Part:BBa_R0040 R0040]
-
|align="center"|
+
|align="center"|tetR repressible promoter
|-
|-
|align="center"|MP105
|align="center"|MP105
-
|align="center"|
+
|align="center"|[http://partsregistry.org/Part:BBa_S03154 S03154]
-
|align="center"|
+
|align="center"|B0034(rbs) ->LasI
|-
|-
|align="center"|MP106
|align="center"|MP106
-
|align="center"|
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|align="center"|[http://partsregistry.org/Part:BBa_S03879 S03879]
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|align="center"|
+
|align="center"|B0034(rbs) -> TetR
|-
|-
|align="center"|MP107
|align="center"|MP107
-
|align="center"|
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|align="center"|[http://partsregistry.org/Part:BBa_C0079 C0079]
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|align="center"|
+
|align="center"|lasR activator with LVA Tag
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|-
+
|-  
|align="center"|MP108
|align="center"|MP108
-
|align="center"|
+
|align="center"|[http://partsregistry.org/Part:BBa_C0179 C0179]
-
|align="center"|
+
|align="center"|lasR activator
|-
|-
|align="center"|MP109
|align="center"|MP109
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|align="center"|
+
|align="center"|[http://partsregistry.org/Part:BBa_E0030 E0030]
-
|align="center"|
+
|align="center"|YFP
|-
|-
|align="center"|MP110
|align="center"|MP110
-
|align="center"|
+
|align="center"|[http://partsregistry.org/Part:BBa_E0040 E0040]
-
|align="center"|
+
|align="center"|GFP
-
|-
+
|-  
|align="center"|MP111
|align="center"|MP111
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|align="center"|
+
|align="center"|[http://partsregistry.org/Part:BBa_E1010 E1010]
-
|align="center"|
+
|align="center"|mRFP
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|-
|align="center"|MP116
|align="center"|MP116
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|align="center"|J23100
+
|align="center"|[http://partsregistry.org/Part:BBa_J23100 J23100]
-
|align="center"|
+
|align="center"|Strong constitutive promoter in J61002
|-
|-
|align="center"|MP117
|align="center"|MP117
-
|align="center"|J23107
+
|align="center"|[http://partsregistry.org/Part:BBa_J23107 J23107]
-
|align="center"|
+
|align="center"|Medium constitutive promoter in J61002
|-
|-
|align="center"|MP118
|align="center"|MP118
-
|align="center"|B0015
+
|align="center"|[http://partsregistry.org/Part:BBa_B0015 B0015]
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|align="center"|
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|align="center"|Double terminator
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|align="center"|MP119
|align="center"|MP119
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|align="center"|I0500
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|align="center"|[http://partsregistry.org/Part:BBa_I0500 I0500]
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|align="center"|
+
|align="center"|AraC pBAD
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|-
|align="center"|MP120
|align="center"|MP120
-
|align="center"|B0030
+
|align="center"|[http://partsregistry.org/Part:BBa_B0030 B0030]
-
|align="center"|
+
|align="center"|Strong RBS (Efficiency = 0,6)
|-
|-
|align="center"|MP121
|align="center"|MP121
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|align="center"|E0422
+
|align="center"|[http://partsregistry.org/Part:BBa_E0422 E0422]
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|align="center"|
+
|align="center"|ECFP (RBS+LVA+Term)
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|-
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|align="center"|MP122
+
|align="center"| MP122
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|align="center"|E0840
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|align="center"|[http://partsregistry.org/Part:BBa_E0840 E0840]
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|align="center"|
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|align="center"|gfp tri-part; strong rbs
 +
|
|}
|}
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== Digestion ==
== Digestion ==

Revision as of 14:40, 26 July 2008

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Contents

MiniPreps

  • The protocol of MiniPrep of Promega has been done on all the clones cultivated on the 25th.
Name Biobrick Description
MP100 [http://partsregistry.org/Part:BBa_B0034 B0034] Strongest RBS (Efficiency = 1)
MP101 [http://partsregistry.org/Part:BBa_J23101 J23101] Strongest Constitutive promoter
MP102 [http://partsregistry.org/Part:BBa_J23109 J23109] Weak Constitutive promoter
MP103 [http://partsregistry.org/Part:BBa_R0079 R0079] promoter pLas (lasr & PAI regulated)
MP104 [http://partsregistry.org/Part:BBa_R0040 R0040] tetR repressible promoter
MP105 [http://partsregistry.org/Part:BBa_S03154 S03154] B0034(rbs) ->LasI
MP106 [http://partsregistry.org/Part:BBa_S03879 S03879] B0034(rbs) -> TetR
MP107 [http://partsregistry.org/Part:BBa_C0079 C0079] lasR activator with LVA Tag
MP108 [http://partsregistry.org/Part:BBa_C0179 C0179] lasR activator
MP109 [http://partsregistry.org/Part:BBa_E0030 E0030] YFP
MP110 [http://partsregistry.org/Part:BBa_E0040 E0040] GFP
MP111 [http://partsregistry.org/Part:BBa_E1010 E1010] mRFP
MP116 [http://partsregistry.org/Part:BBa_J23100 J23100] Strong constitutive promoter in J61002
MP117 [http://partsregistry.org/Part:BBa_J23107 J23107] Medium constitutive promoter in J61002
MP118 [http://partsregistry.org/Part:BBa_B0015 B0015] Double terminator
MP119 [http://partsregistry.org/Part:BBa_I0500 I0500] AraC pBAD
MP120 [http://partsregistry.org/Part:BBa_B0030 B0030] Strong RBS (Efficiency = 0,6)
MP121 [http://partsregistry.org/Part:BBa_E0422 E0422] ECFP (RBS+LVA+Term)
MP122 [http://partsregistry.org/Part:BBa_E0840 E0840] gfp tri-part; strong rbs

Digestion

Digestion Mix

10µl of Miniprep (clone 1 or 2)
12.5µl of water
2.5µl of Buffer N°2
0.25µl of BSA 100x
1µl of enzyme 1
1µl of enzyme 2

list of digestion

Name Miniprep Biobrick Enzyme 1 Enzyme 2 Nber tube
D100 MP100 B0034 XbaI PstI 2
EcoRI XbaI 1
SpeI PstI 2
D101 MP101 J23101 SpeI PstI 2
D102 MP102 J23109 SpeI PstI 2
D103 MP103 R0079 SpeI PstI 2
D104 MP104 R0040 SpeI PstI 2
D105 MP105 S03154 SpeI PstI 1
XbaI PstI 2
EcoRI SpeI 1
D106 MP106 J23101 SpeI PstI 1
XbaI PstI 2
EcoRI SpeI 1
D107 MP107 C0079 EcoRI SpeI 1
D108 MP108 C0179 EcoRI SpeI 1
D109 MP109 E0030 EcoRI SpeI 1
D110 MP110 E0040 EcoRI SpeI 1
D111 MP111 E1010 EcoRI SpeI 1
D116 MP116 J23100 SpeI PstI 2
D117 MP117 J23107 SpeI PstI 2
D118 MP118 B0015 EcoRI XbaI 2
D119 MP119 I0500 SpeI PstI 2
D120 MP120 B0030 SpeI PstI 2
EcoRI XbaI 1
XbaI PstI 2
D121 MP121 E0422 XbaI PstI 3
D122 MP122 E0840 XbaI PstI 3

Results of digestions : Electrophoresis

Retrieved from "http://2008.igem.org/Team:Paris/July_26"