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TUDelft/10 October 2008
From 2008.igem.org
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Contents |
October 10th
Growing for Luciferase assay
For our luciferase assay to include temperature of 20ºC, we'll grow all our cultures (K115012 & K115029-36) in fourfold over the weekend at 20oC. All of them are induced by arabinose (although not strictly necessary) and are grown on ampicillin.
Colony PCR BBa_K115035
A lot of colonies formed on the transformant plate of K115035. So much that we doubt everything went right with the transformation, it might be that the antibiotics were not right. To test this, we have two negative controls (Kanamycin and Tetracycline plates) in the transformation of the thermoparts. To get an idea today, we also did a colony PCR and tested it on gel. This looked fine for K115035.
Transformation thermoparts
Parts that were ligated o/n were now used to transform bacteria. K115001 - K115003 are transformed in DH5α, the others (K115008, K115009, K115016, K115017 and K115020) were transformed in TOP10. Negative controls (non-transformed cells) were performed using DB3.1
