Wiki/Team:Warsaw/igem notebook.htm

From 2008.igem.org

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<tdalign=center><h1>Table of contents</h1></td><td>&nbsp;</td></tr>
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<td align=center width=70%><h1><p style="font-size: 16px; text-align: center; text-decoration: bold;">Table of contents </p></h1></td><td>&nbsp;</td></tr>
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<h3 class="toc"><a onClick=switchMenu('one-armed-vect')> Preparation of one-armed bandit vectors</a></h3>
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<h3 onClick=switchMenu('one-armed-vect') onMouseOver="this.style.color='blue';" onMouseOut="this.style.color='black';"> Preparation of one-armed bandit vectors</h3>
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<div name="menu_item" style="display: none" id="one-armed-vect">
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<h3 class="toc"><a OnClick=switchMenu('one-armed-tests')> One-armed bandit tests</a></h3>
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<h3 OnClick=switchMenu('one-armed-tests') onMouseOver="this.style.color='blue';" onMouseOut="this.style.color='black';"> One-armed bandit tests</h3>
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<div name="menu_item" style="display: none" id="one-armed-tests">
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<h3 class="toc"><a OnClick=switchMenu('hunter-vect')>Preparation of vectors for 'hunter' part of 'hunter and prey'</a></h3>
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<h3 OnClick=switchMenu('hunter-vect') onMouseOver="this.style.color='blue';" onMouseOut="this.style.color='black';">Preparation of vectors for 'hunter' part of 'hunter and prey'</h3>
<div name="menu_item" id="hunter-vect" style="display: none">
<div name="menu_item" id="hunter-vect" style="display: none">
<ol>
<ol>
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<li class="nb_toc1"><a OnClick=switchMenu('prot_A','submenu_item')>Preparation of vectors with protein A derivatives fusions</a></li>
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<li class="nb_toc1" OnClick=switchMenu('prot_A','submenu_item')>Preparation of vectors with protein A derivatives fusions</li>
<div name="submenu_item" id="prot_A" style="display: none">
<div name="submenu_item" id="prot_A" style="display: none">
<ul>
<ul>
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=9&arg0=7_July_2008&arg1=8_July_2008&arg2=14_July_2008&arg3=15_July_2008&arg4=16_July_2008&arg5=21_July_2008&arg6=22_July_2008&arg7=23_July_2008&arg8=28_July_2008&name=Cloning%20omega-A%20fusion%20on%20pKS%20%28second%20attempt%29>Cloning omega-A fusion on pKS (second attempt)</a></li>
<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=9&arg0=7_July_2008&arg1=8_July_2008&arg2=14_July_2008&arg3=15_July_2008&arg4=16_July_2008&arg5=21_July_2008&arg6=22_July_2008&arg7=23_July_2008&arg8=28_July_2008&name=Cloning%20omega-A%20fusion%20on%20pKS%20%28second%20attempt%29>Cloning omega-A fusion on pKS (second attempt)</a></li>
<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=4&arg0=9_July_2008&arg1=10_July_2008&arg2=11_July_2008&arg3=12_July_2008&name=Preparation%20of%20construct%20pKS%20with%20A%20protein>Preparation of construct pKS with A protein </a></li>
<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=4&arg0=9_July_2008&arg1=10_July_2008&arg2=11_July_2008&arg3=12_July_2008&name=Preparation%20of%20construct%20pKS%20with%20A%20protein>Preparation of construct pKS with A protein </a></li>
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=2&arg0=14_July_2008&arg1=15_July_2008&name=Preparation%20of%20alpha%20A%20conctruct>Preparation of alpha A conctruct </a></li>
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=5&arg0=21_July_2008&arg1=22_July_2008&arg2=23_July_2008&arg3=24_July_2008&arg4=25_July_2008&name=Cloning%20of%20protein%20A%20DNA%20to%20OmpA%20constructs>Cloning of protein A DNA to OmpA constructs </a></li>
<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=5&arg0=21_July_2008&arg1=22_July_2008&arg2=23_July_2008&arg3=24_July_2008&arg4=25_July_2008&name=Cloning%20of%20protein%20A%20DNA%20to%20OmpA%20constructs>Cloning of protein A DNA to OmpA constructs </a></li>
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=3&arg0=29_July_2008&arg1=30_July_2008&arg2=31_July_2008&name=Cloning%20of%20omega_%26Delta%3BA%20DNA%20fragment%20to%20pACYC177%20OmpA_alpha>Cloning of omega_&Delta;A DNA fragment to pACYC177 OmpA_alpha </a></li>
 
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=5&arg0=30_July_2008&arg1=31_July_2008&arg2=1_August_2008&arg3=4_August_2008&arg4=5_August_2008&name=Cloning%20of%20truncated%20fragment%20of%20protein%20A%20%28%26Delta%3BA%29>Cloning of truncated fragment of protein A (&Delta;A) </a></li>
 
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=5&arg0=18_August_2008&arg1=19_August_2008&arg2=20_August_2008&arg3=21_August_2008&arg4=22_August_2008&name=Cloning%20of%20protein%20A%20DNA%20to%20GeneArt%20plasmid>Cloning of protein A DNA to GeneArt plasmid </a></li>
 
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<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=4&amp;arg0=4_August_2008&amp;arg1=5_August_2008&amp;arg2=6_August_2008&amp;arg3=7_August_2008&amp;name=Preparing%20pACYC177%20OmpA_omega_%26Delta%3BA%20construct">Preparing pACYC177 OmpA_omega_&Delta;A construct</a>
 
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</li>
 
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=5&amp;arg0=18_August_2008&amp;arg1=19_August_2008&amp;arg2=20_August_2008&amp;arg3=21_August_2008&amp;arg4=22_August_2008&amp;name=Cloning%20of%20protein%20A%20DNA%20to%20GeneArt%20plasmid">Cloning of protein A DNA to GeneArt plasmid</a></li>
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=5&amp;arg0=18_August_2008&amp;arg1=19_August_2008&amp;arg2=20_August_2008&amp;arg3=21_August_2008&amp;arg4=22_August_2008&amp;name=Cloning%20of%20protein%20A%20DNA%20to%20GeneArt%20plasmid">Cloning of protein A DNA to GeneArt plasmid</a></li>
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<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=7&amp;arg0=22_September_2008&amp;arg1=23_September_2008&amp;arg2=24_September_2008&amp;arg3=25_September_2008&amp;arg4=26_September_2008&amp;arg5=27_September_2008&amp;arg6=30_September_2008&amp;name=Mutagenesis%20of%20protein%20A">Mutagenesis of protein A</a></li>
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=7&amp;arg0=22_September_2008&amp;arg1=23_September_2008&amp;arg2=24_September_2008&amp;arg3=25_September_2008&amp;arg4=26_September_2008&amp;arg5=27_September_2008&amp;arg6=30_September_2008&amp;name=Mutagenesis%20of%20protein%20A">Mutagenesis of protein A</a></li>
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<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=2&amp;arg0=24_September_2008&amp;arg1=25_September_2008&amp;name=Preparation%20of%20alpha_A%20construct">Preparation of alpha_A construct</a></li>
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=2&arg0=14_July_2008&arg1=15_July_2008&name=Preparation%20of%20alpha%20A%20conctruct>Preparation of alpha A construct #1</a></li>
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 +
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=2&amp;arg0=24_September_2008&amp;arg1=25_September_2008&amp;name=Preparation%20of%20alpha_A%20construct">Preparation of alpha_A construct #2</a></li>
 +
 
-
<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=4&arg0=4_August_2008&arg1=5_August_2008&arg2=6_August_2008&arg3=7_August_2008&name=Preparing%20pACYC177%20OmpA_omega_%26Delta%3BA%20construct>Preparing pACYC177 OmpA_omega_&Delta;A construct </a></li>
 
</ul>
</ul>
</div>
</div>
 +
<li class="nb_toc1" OnClick=switchMenu('delta_A','submenu_item')>Preparation of vectors with B-domain of protein A derivatives fusions</li>
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<div name="submenu_item" id="delta_A" style="display: none">
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<ul>
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=5&arg0=30_July_2008&arg1=31_July_2008&arg2=1_August_2008&arg3=4_August_2008&arg4=5_August_2008&name=Cloning%20of%20truncated%20fragment%20of%20protein%20A%20%28%26Delta%3BA%29>Cloning of truncated fragment of protein A (&Delta;A) </a></li>
-
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=9&amp;arg0=13_August_2008&amp;arg1=14_August_2008&amp;arg2=18_August_2008&amp;arg3=19_August_2008&amp;arg4=20_August_2008&amp;arg5=21_August_2008&amp;arg6=22_August_2008&amp;arg7=25_August_2008&amp;arg8=26_August_2008&amp;name=Cloning%20of%20alpha%20to%20pACYC177%20OmpA_A_omega%20and%20pACYC177%20OmpA_Z_omega">Cloning of alpha to pACYC177 OmpA_A_omega and pACYC177 OmpA_Z_omega</a></li>
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=3&arg0=29_July_2008&arg1=30_July_2008&arg2=31_July_2008&name=Cloning%20of%20omega_%26Delta%3BA%20DNA%20fragment%20to%20pACYC177%20OmpA_alpha>Cloning of omega_&Delta;A DNA fragment to pACYC177 OmpA_alpha </a></li>
 +
<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=4&arg0=4_August_2008&arg1=5_August_2008&arg2=6_August_2008&arg3=7_August_2008&name=Preparing%20pACYC177%20OmpA_omega_%26Delta%3BA%20construct>Preparing pACYC177 OmpA_omega_&Delta;A construct </a></li>
 +
</ul>
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</div>
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=6&arg0=14_July_2008&arg1=15_July_2008&arg2=16_July_2008&arg3=17_July_2008&arg4=18_July_2008&arg5=19_July_2008&name=Cloning%20of%20protein%20Z%20DNA%20to%20OmpA%20constructs>Cloning of protein Z DNA to OmpA constructs </a></li>
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<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=9&amp;arg0=13_August_2008&amp;arg1=14_August_2008&amp;arg2=18_August_2008&amp;arg3=19_August_2008&amp;arg4=20_August_2008&amp;arg5=21_August_2008&amp;arg6=22_August_2008&amp;arg7=25_August_2008&amp;arg8=26_August_2008&amp;name=Cloning%20of%20alpha%20to%20pACYC177%20OmpA_A_omega%20and%20pACYC177%20OmpA_Z_omega">Cloning of alpha to pACYC177 OmpA_A_omega and pACYC177 OmpA_Z_omega</a></li>
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<h3 class="toc"><a OnClick=switchMenu('prey-vect')>Preparation of vectors for 'prey' part of 'hunter and prey'</a></h3>
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<h3 OnClick=switchMenu('prey-vect') onMouseOver="this.style.color='blue';" onMouseOut="this.style.color='black';">Preparation of vectors for 'prey' part of 'hunter and prey'</h3>
<div name="menu_item" id="prey-vect" style="display: none">
<div name="menu_item" id="prey-vect" style="display: none">
<ol>
<ol>
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=7&arg0=17_July_2008&arg1=18_July_2008&arg2=21_July_2008&arg3=22_July_2008&arg4=23_July_2008&arg5=24_July_2008&arg6=25_July_2008&name=Cloning%20of%20protein%20Z%20DNA%20to%20pET15b-OmpA-omega%20in%20place%20of%20OmpA>Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA </a></li>
<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=7&arg0=17_July_2008&arg1=18_July_2008&arg2=21_July_2008&arg3=22_July_2008&arg4=23_July_2008&arg5=24_July_2008&arg6=25_July_2008&name=Cloning%20of%20protein%20Z%20DNA%20to%20pET15b-OmpA-omega%20in%20place%20of%20OmpA>Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA </a></li>
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=7&arg0=19_August_2008&arg1=22_August_2008&arg2=25_August_2008&arg3=26_August_2008&arg4=27_August_2008&arg5=28_August_2008&arg6=29_August_2008&name=Cloning%20of%20protein%20A%20DNA%20to%20pET15b%20OmpA-alpha%20plasmid>Cloning of protein A DNA to pET15b OmpA-alpha plasmid </a></li>
 
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=6&arg0=14_July_2008&arg1=15_July_2008&arg2=16_July_2008&arg3=17_July_2008&arg4=18_July_2008&arg5=19_July_2008&name=Cloning%20of%20protein%20Z%20DNA%20to%20OmpA%20constructs>Cloning of protein Z DNA to OmpA constructs </a></li>
 
<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=4&arg0=9_July_2008&arg1=10_July_2008&arg2=11_July_2008&arg3=12_July_2008&name=Cloning%20of%20protein%20Z%20DNA%20to%20pET15b-OmpA-alpha%20in%20place%20of%20OmpA>Cloning of protein Z DNA to pET15b-OmpA-alpha in place of OmpA </a></li>
<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=4&arg0=9_July_2008&arg1=10_July_2008&arg2=11_July_2008&arg3=12_July_2008&name=Cloning%20of%20protein%20Z%20DNA%20to%20pET15b-OmpA-alpha%20in%20place%20of%20OmpA>Cloning of protein Z DNA to pET15b-OmpA-alpha in place of OmpA </a></li>
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<h3 class="toc"><a OnClick=switchMenu('prey-purif')>Purification of prey proteins</a></h3>
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<h3 OnClick=switchMenu('prey-purif') onMouseOver="this.style.color='blue';" onMouseOut="this.style.color='black';">Purification of prey proteins</h3>
<div name="menu_item" id="prey-purif" style="display: none">
<div name="menu_item" id="prey-purif" style="display: none">
<ol>
<ol>
<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=9&arg0=28_July_2008&arg1=29_July_2008&arg2=30_July_2008&arg3=31_July_2008&arg4=7_August_2008&arg5=11_August_2008&arg6=12_August_2008&arg7=14_August_2008&arg8=18_August_2008&name=Purification%20of%20proteins%3A%20Z-alpha%20and%20Z-omega>Purification of proteins: Z-alpha and Z-omega </a></li>
<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=9&arg0=28_July_2008&arg1=29_July_2008&arg2=30_July_2008&arg3=31_July_2008&arg4=7_August_2008&arg5=11_August_2008&arg6=12_August_2008&arg7=14_August_2008&arg8=18_August_2008&name=Purification%20of%20proteins%3A%20Z-alpha%20and%20Z-omega>Purification of proteins: Z-alpha and Z-omega </a></li>
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<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=8&amp;arg0=29_August_2008&
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<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=8&amp;arg0=29_August_2008&amp;arg1=1_September_2008&amp;arg2=2_September_2008&amp;arg3=3_September_2008&amp;arg4=4_September_2008&amp;arg5=5_September_2008&amp;arg6=8_September_2008&amp;arg7=9_September_2008&amp;name=Purification%20of%20proteins%3A%20A-alpha">Purification of proteins: A-alpha</a></li>
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amp;arg1=1_September_2008&amp;arg2=2_September_2008&amp;arg3=3_September_2008&amp;arg4=4_September_2008&amp;arg5=5_September_2008&amp;arg6=8_September_2008&amp;arg7=9_September_2008&amp;name=Purification%20of%20proteins%3A%20A-alpha">Purification of proteins: A-alpha</a></li>
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<h3 class="toc"><a OnClick="switchMenu('hunter-tests')">'Hunter and prey' tests</h3>
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<h3 OnClick="switchMenu('hunter-tests')" onMouseOver="this.style.color='blue';" onMouseOut="this.style.color='black';">'Hunter and prey' tests</h3>
<div name="menu_item" id="hunter-tests" style="display: none">
<div name="menu_item" id="hunter-tests" style="display: none">
<ol>
<ol>
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<li class="nb_toc1"><a OnClick="switchMenu('expr-tests','submenu_item')">Protein expression tests</li>
 
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<div name="submenu_item" id="expr-tests" style="display: none">
 
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<ul>
 
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=1&arg0=4_August_2008&name=Checking%20OmpA_omega_%26Delta%3BA_alpha%20and%20OmpA_A_alpha%20expression>Checking OmpA_omega_&Delta;A_alpha and OmpA_A_alpha expression </a></li>
 
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=1&arg0=5_August_2008&name=Checking%20OmpA_omega_%26Delta%3BA_alpha%20expression>Checking OmpA_omega_&Delta;A_alpha expression </a></li>
 
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</ul>
 
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</div>
 
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<li class="nb_toc1"><a OnClick="switchMenu('resist-tests','submenu_item')">Ampicillin-resistance tests</li>
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<li class="nb_toc1" OnClick="switchMenu('resist-tests','submenu_item')">Ampicillin-resistance tests</li>
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<div name="submenu_item" id="resist-tests" style="display: none">
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=2&arg0=4_August_2008&arg1=5_August_2008&name=Checking%20OmpA_omega_%26Delta%3BA_alpha%20expression>Checking OmpA_omega_ΔA_alpha expression</a></li>
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<h3 class="toc"><a OnClick=switchMenu('bricks')>Preparation of BioBricks</a></h3>
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<h3 OnClick=switchMenu('bricks') onMouseOver="this.style.color='blue';" onMouseOut="this.style.color='black';">Preparation of BioBricks</h3>
<div name="menu_item" id="bricks" style="display: none">
<div name="menu_item" id="bricks" style="display: none">
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<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=2&amp;arg0=17_September_2008&amp;arg1=18_September_2008&amp;name=Optimisation%20of%20primers%20for%20preparation%20of%20parts">Optimisation of primers for preparation of parts</a></li>
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=2&amp;arg0=17_September_2008&amp;arg1=18_September_2008&amp;name=Optimisation%20of%20primers%20for%20preparation%20of%20parts">Optimisation of primers for preparation of parts</a></li>
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<li class="nb_toc1"><a OnClick=switchMenu('bricks-vect','submenu_item')>Preparation of vectors for BioBricks parts cloning</a></li>
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<li class="nb_toc1" OnClick=switchMenu('bricks-vect','submenu_item')>Preparation of vectors for BioBricks parts cloning</li>
<div name="submenu_item" id="bricks-vect" style="display: none">
<div name="submenu_item" id="bricks-vect" style="display: none">
<ul>
<ul>
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</div>
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<li class="nb_toc1"><a OnClick=switchMenu('bricks-coding','submenu_item')>Preparation of BioBricks parts (coding type)</a></li>
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<li class="nb_toc1" OnClick=switchMenu('bricks-coding','submenu_item')>Preparation of BioBricks parts (coding type)</li>
<div name="submenu_item" id="bricks-coding" style="display: none">
<div name="submenu_item" id="bricks-coding" style="display: none">
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</div>
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<li class="nb_toc1"><a OnClick=switchMenu('bricks-generator','submenu_item')>Preparation of BioBricks parts (coding type)</a></li>
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<li class="nb_toc1" OnClick=switchMenu('bricks-generator','submenu_item')>Preparation of BioBricks parts (coding type)</li>
<div name="submenu_item" id="bricks-generator" style="display: none">
<div name="submenu_item" id="bricks-generator" style="display: none">
<ul>
<ul>
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<h3 class="toc"><a OnClick=switchMenu('other')>Other activities</a></h3>
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<h3 OnClick=switchMenu('other') onMouseOver="this.style.color='blue';" onMouseOut="this.style.color='black';">Other activities</h3>
<div name="menu_item" id="other" style="display: none">
<div name="menu_item" id="other" style="display: none">
<ol>
<ol>
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<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=1&amp;arg0=8_September_2008&amp;name=Our%20webpage%20has%20a%20completely%20new%20layout">Our webpage has a completely new layout</a></li>
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=1&amp;arg0=8_September_2008&amp;name=Our%20webpage%20has%20a%20completely%20new%20layout">Our webpage has a completely new layout</a></li>
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=1&amp;arg0=11_September_2008&amp;name=We%27re%20fighting%20hard%20to%20obtain%20funds%20for%20the%20visit%20on%20iGEM2008%20Jamboree%21">We're fighting hard to obtain funds for the visit on iGEM2008 Jamboree!</a></li>
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=1&amp;arg0=11_September_2008&amp;name=We%27re%20fighting%20hard%20to%20obtain%20funds%20for%20the%20visit%20on%20iGEM2008%20Jamboree%21">We're fighting hard to obtain funds for the visit on iGEM2008 Jamboree!</a></li>
 +
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=1&amp;arg0=10_October_2008&amp;name=Visit%20in%20US%20Embassy">Visit in US Embassy</a></li>
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=1&amp;arg0=23_October_2008&amp;name=Sending%20our%20bricks%20to%20the%20Registry">Sending our bricks to the Registry</a></li>
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=1&amp;arg0=23_October_2008&amp;name=Sending%20our%20bricks%20to%20the%20Registry">Sending our bricks to the Registry</a></li>
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=1&amp;arg0=24_October_2008&amp;name=Computer%20meeting%20to%20finish%20our%20webpage">Computer meeting to finish our webpage</a></li>
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=1&amp;arg0=24_October_2008&amp;name=Computer%20meeting%20to%20finish%20our%20webpage">Computer meeting to finish our webpage</a></li>
-
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=1&amp;arg0=10_October_2008&amp;name=Visit%20in%20US%20Embassy">Visit in US Embassy</a></li>
+
 
</ol>
</ol>
</div>
</div>
 +
<br>
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|{{#calendar: query=preload=Team:Warsaw/NotebookPreload | year=2008 | month=09 | title=Team:Warsaw/Calendar-Main}}
|{{#calendar: query=preload=Team:Warsaw/NotebookPreload | year=2008 | month=09 | title=Team:Warsaw/Calendar-Main}}
|{{#calendar: query=preload=Team:Warsaw/NotebookPreload | year=2008 | month=10 | title=Team:Warsaw/Calendar-Main}}
|{{#calendar: query=preload=Team:Warsaw/NotebookPreload | year=2008 | month=10 | title=Team:Warsaw/Calendar-Main}}
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|{{#calendar: query=preload=Team:Warsaw/NotebookPreload | year=2008 | month=11 | title=Team:Warsaw/Calendar-Main}}
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</td></tr></table>
</td></tr></table>
 +
<hr width="100%">
-
<h2 align="left"><a href=https://2008.igem.org/Wiki/Team:Warsaw/protocols_list>Protocols</a></h2>
+
<h3 align="left" OnClick="switchMenu('protocols','menu_item')" onMouseOver="this.style.color='blue';" onMouseOut="this.style.color='black';">Protocols</h3>
 +
<div name="menu_item" id="protocols" style="display:none">
 +
 
 +
<ol>
 +
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#Z_a_Z_o">Purification of His_Z_alpha and His_Z_omega</a></li>
 +
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#A_a">Purification of His_A_alpha</a></li>
 +
 
 +
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#Testing various hunter/prey combinations">Testing various hunter/prey combinations</a></li>
 +
 
 +
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Plasmid DNA isolation</a></li>
 +
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">DNA isolation from agarose gel</a></li>
 +
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_purification_after_enzymatic_reaction">DNA purification after enzymatic reaction</a></li>
 +
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#genomic_DNA_isolation">Genomic DNA isolation</a></li>
 +
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemocompetent">Preparation of chemocompetent bacteria</a></li>
 +
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#electrocompetent">Preparation of electrocompetent bacteria</a></li>
 +
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Chemotransformation</a></li>
 +
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#electrotransform">Electrotransformation</a></li>
 +
 
 +
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a></li>
 +
 
 +
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#blunting">Blunting</a></li>
 +
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#concentrations">Standard concentrations of antibiotics and other supplements
 +
</a></li>
 +
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#rif">Rifampicin test
 +
</a></li>
 +
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#removing">Removing 5' phosphate groups from DNA ends
 +
</a></li>
 +
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#taxi">TAXI protocol (Tet+Ap 30+X-Gal+IPTG)
 +
</a></li>
 +
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#bca">Protein concentration measurement (BCA method)
 +
</a></li>
 +
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">DNA digest
 +
</a></li>
 +
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR
 +
</a></li>
 +
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcl">Polymerase Chain Ligation (PCL)
 +
</a></li>
 +
 
 +
</ol>
 +
</div>
-
<h2 align="left"><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/primers">Primers</a></h2>
+
<h3 align="left"><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/primers">Primers</a></h3>
-
<h2 align="left"><a href="https://2008.igem.org/Wiki/Team:Warsaw/vectors">Vectors</a></h2>
+
<h3 align="left"><a href="https://2008.igem.org/Wiki/Team:Warsaw/vectors">Vectors</a></h3>
-
<h2 align="left"><a href="https://2008.igem.org/Wiki/Team:Warsaw/scripts">JavaScript tools developed to make working with iGEM notebook more comfortable</a></h2>
+
<h3 align="left" OnClick="switchMenu('strains','menu_item')" onMouseOver="this.style.color='blue';" onMouseOut="this.style.color='black';">Strains</h3>
-
<h2 align="left">Strains</h2>
+
<div name="menu_item" id="strains" style="display:none;">
<h4><a name="rosetta">Rosetta(DE3)pLysS</a></h4>
<h4><a name="rosetta">Rosetta(DE3)pLysS</a></h4>
<p>F- ompT hsdSB(RB- mB-) gal dcm &lambda;(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5]) pLysSRARE (CamR)</p>
<p>F- ompT hsdSB(RB- mB-) gal dcm &lambda;(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5]) pLysSRARE (CamR)</p>
<h4><a name="top10">TOP10 (Invitrogen)</a></h4>
<h4><a name="top10">TOP10 (Invitrogen)</a></h4>
<p>F- mcrA &Delta;(mrr-hsdRMS-mcrBC) &phi;80lacZ&Delta;M15 &Delta;lacX74 nupG recA1 araD139 &Delta;(ara-leu)7697 galE15 galK16 rpsL(StrR) endA1 &lambda;- </p>
<p>F- mcrA &Delta;(mrr-hsdRMS-mcrBC) &phi;80lacZ&Delta;M15 &Delta;lacX74 nupG recA1 araD139 &Delta;(ara-leu)7697 galE15 galK16 rpsL(StrR) endA1 &lambda;- </p>
-
<h4><a name="mutD5">NR9458 (IBB collection; in notebook called mutD5)</a></h4>
+
<h4><a name="mutD5">NR9458 (<a href="http://www.ibb.waw.pl/">Institute of Biochemistry and Biophysics</a> collection; in notebook called mutD5)</a></h4>
<p>ara thi &Delta; pro lac mutD5 zaf-13 ::Tn10</p>
<p>ara thi &Delta; pro lac mutD5 zaf-13 ::Tn10</p>
<h4><a name="gm">GM2163</a></h4>
<h4><a name="gm">GM2163</a></h4>
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R(zgb210::Tn10)TetS endA1 rpsL136 dam13::Tn9 xylA-5 mtl-1 thi-1 mcrB1
R(zgb210::Tn10)TetS endA1 rpsL136 dam13::Tn9 xylA-5 mtl-1 thi-1 mcrB1
hsdR2</p>
hsdR2</p>
-
 
+
</div>
-
</div></td>
+
<h3 align="left" OnClick="switchMenu('javascript','menu_item')" onMouseOver="this.style.color='blue';" onMouseOut="this.style.color='black';">JavaScript tools developed to make work with iGEM notebook more comfortable</h3>
 +
<div name="menu_item" id="javascript" style="display: none">
 +
<ol>
 +
<li><a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/NoAtribution">Author attribution analyzer</a></li>
 +
<li><a href="https://2008.igem.org/Team:Warsaw/JSTest2">Inretactive Notebook topics list</a></li>
 +
</ol>
 +
</div>
 +
</td>
   <td><img src="https://static.igem.org/mediawiki/2008/5/52/Spacer.gif" width="1" height="530" border="0" alt="" /></td>
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else {
else {
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document.getElementById(obj).style.display = 'block';
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qs=parent.document.URL;
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//-->
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</script>
</script>
</html>
</html>

Latest revision as of 06:51, 30 October 2008

Gallery Bricks Notebook Team Project Home

Table of contents

 

Preparation of one-armed bandit vectors

One-armed bandit tests

Preparation of vectors for 'hunter' part of 'hunter and prey'

Preparation of vectors for 'prey' part of 'hunter and prey'

Purification of prey proteins

'Hunter and prey' tests

Preparation of BioBricks

Other activities

May
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
June
MTWTFSS
            1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30
July
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30 31
August
MTWTFSS
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
September
MTWTFSS
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30
October
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31

Protocols

Primers

Vectors

Strains

JavaScript tools developed to make work with iGEM notebook more comfortable
Retrieved from "http://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm"