From 2008.igem.org

(Difference between revisions)

@@ Line 8: / Line 8: @@
 <table class="month"><tr>
-<td align=center width=70%><h1><p style="font-size: 16px; text-align: center; text-decoration: bold;">Table of contents</p></h1></td><td>&nbsp;</td></tr>
+<td align=center width=70%><h1><p style="font-size: 16px; text-align: center; text-decoration: bold;">Table of contents </p></h1></td><td>&nbsp;</td></tr>
 <td width=70% valign=top style="border-left-style: grooved">
 <h3 onClick=switchMenu('one-armed-vect') onMouseOver="this.style.color='blue';" onMouseOut="this.style.color='black';"> Preparation of one-armed bandit vectors</h3>
@@ Line 60: / Line 60: @@
 <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=9&arg0=7_July_2008&arg1=8_July_2008&arg2=14_July_2008&arg3=15_July_2008&arg4=16_July_2008&arg5=21_July_2008&arg6=22_July_2008&arg7=23_July_2008&arg8=28_July_2008&name=Cloning%20omega-A%20fusion%20on%20pKS%20%28second%20attempt%29>Cloning omega-A fusion on pKS (second attempt)</a></li>
 <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=4&arg0=9_July_2008&arg1=10_July_2008&arg2=11_July_2008&arg3=12_July_2008&name=Preparation%20of%20construct%20pKS%20with%20A%20protein>Preparation of construct pKS with A protein </a></li>
-<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=2&arg0=14_July_2008&arg1=15_July_2008&name=Preparation%20of%20alpha%20A%20conctruct>Preparation of alpha A conctruct </a></li>
-<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=5&arg0=21_July_2008&arg1=22_July_2008&arg2=23_July_2008&arg3=24_July_2008&arg4=25_July_2008&name=Cloning%20of%20protein%20A%20DNA%20to%20OmpA%20constructs>Cloning of protein A DNA to OmpA constructs </a></li>
-<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=5&arg0=18_August_2008&arg1=19_August_2008&arg2=20_August_2008&arg3=21_August_2008&arg4=22_August_2008&name=Cloning%20of%20protein%20A%20DNA%20to%20GeneArt%20plasmid>Cloning of protein A DNA to GeneArt plasmid </a></li>
+<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=5&arg0=21_July_2008&arg1=22_July_2008&arg2=23_July_2008&arg3=24_July_2008&arg4=25_July_2008&name=Cloning%20of%20protein%20A%20DNA%20to%20OmpA%20constructs>Cloning of protein A DNA to OmpA constructs </a></li>
@@ Line 72: / Line 68: @@
 <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=7&amp;arg0=22_September_2008&amp;arg1=23_September_2008&amp;arg2=24_September_2008&amp;arg3=25_September_2008&amp;arg4=26_September_2008&amp;arg5=27_September_2008&amp;arg6=30_September_2008&amp;name=Mutagenesis%20of%20protein%20A">Mutagenesis of protein A</a></li>
-<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=2&amp;arg0=24_September_2008&amp;arg1=25_September_2008&amp;name=Preparation%20of%20alpha_A%20construct">Preparation of alpha_A construct</a></li>
+<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=2&arg0=14_July_2008&arg1=15_July_2008&name=Preparation%20of%20alpha%20A%20conctruct>Preparation of alpha A construct #1</a></li>
+<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=2&amp;arg0=24_September_2008&amp;arg1=25_September_2008&amp;name=Preparation%20of%20alpha_A%20construct">Preparation of alpha_A construct #2</a></li>
@@ Line 81: / Line 79: @@
 <div name="submenu_item" id="delta_A" style="display: none">
 <ul>
-<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=3&arg0=29_July_2008&arg1=30_July_2008&arg2=31_July_2008&name=Cloning%20of%20omega_%26Delta%3BA%20DNA%20fragment%20to%20pACYC177%20OmpA_alpha>Cloning of omega_&Delta;A DNA fragment to pACYC177 OmpA_alpha </a></li>
 <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=5&arg0=30_July_2008&arg1=31_July_2008&arg2=1_August_2008&arg3=4_August_2008&arg4=5_August_2008&name=Cloning%20of%20truncated%20fragment%20of%20protein%20A%20%28%26Delta%3BA%29>Cloning of truncated fragment of protein A (&Delta;A) </a></li>
+<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=3&arg0=29_July_2008&arg1=30_July_2008&arg2=31_July_2008&name=Cloning%20of%20omega_%26Delta%3BA%20DNA%20fragment%20to%20pACYC177%20OmpA_alpha>Cloning of omega_&Delta;A DNA fragment to pACYC177 OmpA_alpha </a></li>
 <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=4&arg0=4_August_2008&arg1=5_August_2008&arg2=6_August_2008&arg3=7_August_2008&name=Preparing%20pACYC177%20OmpA_omega_%26Delta%3BA%20construct>Preparing pACYC177 OmpA_omega_&Delta;A construct </a></li>
-<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=4&amp;arg0=4_August_2008&amp;arg1=5_August_2008&amp;arg2=6_August_2008&amp;arg3=7_August_2008&amp;name=Preparing%20pACYC177%20OmpA_omega_%26Delta%3BA%20construct">Preparing pACYC177 OmpA_omega_&Delta;A construct</a>
-</li>
 </ul>
 </div>
+<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=6&arg0=14_July_2008&arg1=15_July_2008&arg2=16_July_2008&arg3=17_July_2008&arg4=18_July_2008&arg5=19_July_2008&name=Cloning%20of%20protein%20Z%20DNA%20to%20OmpA%20constructs>Cloning of protein Z DNA to OmpA constructs </a></li>
 <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=9&amp;arg0=13_August_2008&amp;arg1=14_August_2008&amp;arg2=18_August_2008&amp;arg3=19_August_2008&amp;arg4=20_August_2008&amp;arg5=21_August_2008&amp;arg6=22_August_2008&amp;arg7=25_August_2008&amp;arg8=26_August_2008&amp;name=Cloning%20of%20alpha%20to%20pACYC177%20OmpA_A_omega%20and%20pACYC177%20OmpA_Z_omega">Cloning of alpha to pACYC177 OmpA_A_omega and pACYC177 OmpA_Z_omega</a></li>
@@ Line 119: / Line 117: @@
 <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=7&arg0=17_July_2008&arg1=18_July_2008&arg2=21_July_2008&arg3=22_July_2008&arg4=23_July_2008&arg5=24_July_2008&arg6=25_July_2008&name=Cloning%20of%20protein%20Z%20DNA%20to%20pET15b-OmpA-omega%20in%20place%20of%20OmpA>Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA </a></li>
-<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=7&arg0=19_August_2008&arg1=22_August_2008&arg2=25_August_2008&arg3=26_August_2008&arg4=27_August_2008&arg5=28_August_2008&arg6=29_August_2008&name=Cloning%20of%20protein%20A%20DNA%20to%20pET15b%20OmpA-alpha%20plasmid>Cloning of protein A DNA to pET15b OmpA-alpha plasmid </a></li>
-<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=6&arg0=14_July_2008&arg1=15_July_2008&arg2=16_July_2008&arg3=17_July_2008&arg4=18_July_2008&arg5=19_July_2008&name=Cloning%20of%20protein%20Z%20DNA%20to%20OmpA%20constructs>Cloning of protein Z DNA to OmpA constructs </a></li>
 <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=4&arg0=9_July_2008&arg1=10_July_2008&arg2=11_July_2008&arg3=12_July_2008&name=Cloning%20of%20protein%20Z%20DNA%20to%20pET15b-OmpA-alpha%20in%20place%20of%20OmpA>Cloning of protein Z DNA to pET15b-OmpA-alpha in place of OmpA </a></li>
@@ Line 144: / Line 139: @@
 <div name="menu_item" id="hunter-tests" style="display: none">
 <ol>
-<li class="nb_toc1"><a OnClick="switchMenu('expr-tests','submenu_item')">Protein expression tests</li>
-<div name="submenu_item" id="expr-tests" style="display: none">
-<ul>
-<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=1&arg0=4_August_2008&name=Checking%20OmpA_omega_%26Delta%3BA_alpha%20and%20OmpA_A_alpha%20expression>Checking OmpA_omega_&Delta;A_alpha and OmpA_A_alpha expression </a></li>
-<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=1&arg0=5_August_2008&name=Checking%20OmpA_omega_%26Delta%3BA_alpha%20expression>Checking OmpA_omega_&Delta;A_alpha expression </a></li>
-</ul>
-</div>
 <li class="nb_toc1" OnClick="switchMenu('resist-tests','submenu_item')">Ampicillin-resistance tests</li>
@@ Line 163: / Line 151: @@
 </ul>
 </div>
+<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=2&arg0=4_August_2008&arg1=5_August_2008&name=Checking%20OmpA_omega_%26Delta%3BA_alpha%20expression>Checking OmpA_omega_ΔA_alpha expression</a></li>
@@ Line 183: / Line 173: @@
 <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=2&amp;arg0=17_September_2008&amp;arg1=18_September_2008&amp;name=Optimisation%20of%20primers%20for%20preparation%20of%20parts">Optimisation of primers for preparation of parts</a></li>
-<li class="nb_toc1"><a OnClick=switchMenu('bricks-vect','submenu_item')>Preparation of vectors for BioBricks parts cloning</a></li>
+<li class="nb_toc1" OnClick=switchMenu('bricks-vect','submenu_item')>Preparation of vectors for BioBricks parts cloning</li>
 <div name="submenu_item" id="bricks-vect" style="display: none">
 <ul>
@@ Line 193: / Line 183: @@
 </div>
-<li class="nb_toc1" OnClick=switchMenu('bricks-coding','submenu_item')>Preparation of BioBricks parts (coding type)</a></li>
+<li class="nb_toc1" OnClick=switchMenu('bricks-coding','submenu_item')>Preparation of BioBricks parts (coding type)</li>
 <div name="submenu_item" id="bricks-coding" style="display: none">
 <ul>
@@ Line 217: / Line 207: @@
 </div>
-<li class="nb_toc1" OnClick=switchMenu('bricks-generator','submenu_item')>Preparation of BioBricks parts (coding type)</a></li>
+<li class="nb_toc1" OnClick=switchMenu('bricks-generator','submenu_item')>Preparation of BioBricks parts (coding type)</li>
 <div name="submenu_item" id="bricks-generator" style="display: none">
 <ul>
@@ Line 243: / Line 233: @@
 <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=1&amp;arg0=8_September_2008&amp;name=Our%20webpage%20has%20a%20completely%20new%20layout">Our webpage has a completely new layout</a></li>
 <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=1&amp;arg0=11_September_2008&amp;name=We%27re%20fighting%20hard%20to%20obtain%20funds%20for%20the%20visit%20on%20iGEM2008%20Jamboree%21">We're fighting hard to obtain funds for the visit on iGEM2008 Jamboree!</a></li>
+<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=1&amp;arg0=10_October_2008&amp;name=Visit%20in%20US%20Embassy">Visit in US Embassy</a></li>
 <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=1&amp;arg0=23_October_2008&amp;name=Sending%20our%20bricks%20to%20the%20Registry">Sending our bricks to the Registry</a></li>
 <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=1&amp;arg0=24_October_2008&amp;name=Computer%20meeting%20to%20finish%20our%20webpage">Computer meeting to finish our webpage</a></li>
-<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=1&amp;arg0=10_October_2008&amp;name=Visit%20in%20US%20Embassy">Visit in US Embassy</a></li>
 </ol>
 </div>
 <br>
-<hr width="50%">
-<h3 align="left" OnClick="switchMenu('protocols','submenu_item')" onMouseOver="this.style.color='blue';" onMouseOut="this.style.color='black';">Protocols</h3>
-<div name="submenu_item" id="protocols" style="display:none">
+</td><td valign="top" style> </html>
+{| style="background:transparent;"
+|{{#calendar: query=preload=Team:Warsaw/NotebookPreload | year=2008 | month=05 | title=Team:Warsaw/Calendar-Main}}
+|{{#calendar: query=preload=Team:Warsaw/NotebookPreload | year=2008 | month=06 | title=Team:Warsaw/Calendar-Main}}
+|-
+|{{#calendar: query=preload=Team:Warsaw/NotebookPreload | year=2008 | month=07 | title=Team:Warsaw/Calendar-Main}}
+|{{#calendar: query=preload=Team:Warsaw/NotebookPreload | year=2008 | month=08 | title=Team:Warsaw/Calendar-Main}}
+|-
+|{{#calendar: query=preload=Team:Warsaw/NotebookPreload | year=2008 | month=09 | title=Team:Warsaw/Calendar-Main}}
+|{{#calendar: query=preload=Team:Warsaw/NotebookPreload | year=2008 | month=10 | title=Team:Warsaw/Calendar-Main}}
+|}<html>
+</td></tr></table>
+<hr width="100%">
+<h3 align="left" OnClick="switchMenu('protocols','menu_item')" onMouseOver="this.style.color='blue';" onMouseOut="this.style.color='black';">Protocols</h3>
+<div name="menu_item" id="protocols" style="display:none">
 <ol>
@@ Line 294: / Line 300: @@
 <h3 align="left"><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/primers">Primers</a></h3>
 <h3 align="left"><a href="https://2008.igem.org/Wiki/Team:Warsaw/vectors">Vectors</a></h3>
-<h3 align="left"><a href="https://2008.igem.org/Wiki/Team:Warsaw/scripts">JavaScript tools developed to make work with iGEM notebook more comfortable</a></h3>
+<h3 align="left" OnClick="switchMenu('strains','menu_item')" onMouseOver="this.style.color='blue';" onMouseOut="this.style.color='black';">Strains</h3>
-<h3 align="left">Strains</h3>
+<div name="menu_item" id="strains" style="display:none;">
 <h4><a name="rosetta">Rosetta(DE3)pLysS</a></h4>
 <p>F- ompT hsdSB(RB- mB-) gal dcm &lambda;(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5]) pLysSRARE (CamR)</p>
 <h4><a name="top10">TOP10 (Invitrogen)</a></h4>
 <p>F- mcrA &Delta;(mrr-hsdRMS-mcrBC) &phi;80lacZ&Delta;M15 &Delta;lacX74 nupG recA1 araD139 &Delta;(ara-leu)7697 galE15 galK16 rpsL(StrR) endA1 &lambda;- </p>
-<h4><a name="mutD5">NR9458 (IBB collection; in notebook called mutD5)</a></h4>
+<h4><a name="mutD5">NR9458 (<a href="http://www.ibb.waw.pl/">Institute of Biochemistry and Biophysics</a> collection; in notebook called mutD5)</a></h4>
 <p>ara thi &Delta; pro lac mutD5 zaf-13 ::Tn10</p>
 <h4><a name="gm">GM2163</a></h4>
@@ Line 306: / Line 312: @@
 R(zgb210::Tn10)TetS endA1 rpsL136 dam13::Tn9 xylA-5 mtl-1 thi-1 mcrB1
 hsdR2</p>
+</div>
+<h3 align="left" OnClick="switchMenu('javascript','menu_item')" onMouseOver="this.style.color='blue';" onMouseOut="this.style.color='black';">JavaScript tools developed to make work with iGEM notebook more comfortable</h3>
+<div name="menu_item" id="javascript" style="display: none">
-</td><td valign="top" style> </html>
+<ol>
-{| style="background:transparent;"
+<li><a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/NoAtribution">Author attribution analyzer</a></li>
-|{{#calendar: query=preload=Team:Warsaw/NotebookPreload | year=2008 | month=05 | title=Team:Warsaw/Calendar-Main}}
+<li><a href="https://2008.igem.org/Team:Warsaw/JSTest2">Inretactive Notebook topics list</a></li>
-|{{#calendar: query=preload=Team:Warsaw/NotebookPreload | year=2008 | month=06 | title=Team:Warsaw/Calendar-Main}}
+</ol>
-|-
+</div>
-|{{#calendar: query=preload=Team:Warsaw/NotebookPreload | year=2008 | month=07 | title=Team:Warsaw/Calendar-Main}}
+</td>
-|{{#calendar: query=preload=Team:Warsaw/NotebookPreload | year=2008 | month=08 | title=Team:Warsaw/Calendar-Main}}
-|-
-|{{#calendar: query=preload=Team:Warsaw/NotebookPreload | year=2008 | month=09 | title=Team:Warsaw/Calendar-Main}}
-|{{#calendar: query=preload=Team:Warsaw/NotebookPreload | year=2008 | month=10 | title=Team:Warsaw/Calendar-Main}}
-|}<html>
-</td></tr></table>
- </div></td>
     <td><img src="https://static.igem.org/mediawiki/2008/5/52/Spacer.gif" width="1" height="530" border="0" alt="" /></td>
    </tr>
@@ Line 376: / Line 372: @@
 	}
+}
+qs=parent.document.URL;
-}
+if (qs.indexOf('#')>0) document.getElementById("strains").style.display = 'block';
 //-->
 </script>
 </html>

Wiki/Team:Warsaw/igem notebook.htm

From 2008.igem.org

Latest revision as of 06:51, 30 October 2008

Table of contents

Preparation of one-armed bandit vectors

One-armed bandit tests

Preparation of vectors for 'hunter' part of 'hunter and prey'

Preparation of vectors for 'prey' part of 'hunter and prey'

Purification of prey proteins

'Hunter and prey' tests

Preparation of BioBricks

Other activities

Protocols

Primers

Vectors

Strains

Rosetta(DE3)pLysS

TOP10 (Invitrogen)

NR9458 (Institute of Biochemistry and Biophysics collection; in notebook called mutD5)

GM2163

JavaScript tools developed to make work with iGEM notebook more comfortable