Wiki/Team:Warsaw/igem notebook.htm

From 2008.igem.org

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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=2&arg0=14_July_2008&arg1=15_July_2008&name=Preparation%20of%20alpha%20A%20conctruct>Preparation of alpha A conctruct </a></li>
<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=2&arg0=14_July_2008&arg1=15_July_2008&name=Preparation%20of%20alpha%20A%20conctruct>Preparation of alpha A conctruct </a></li>
<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=5&arg0=21_July_2008&arg1=22_July_2008&arg2=23_July_2008&arg3=24_July_2008&arg4=25_July_2008&name=Cloning%20of%20protein%20A%20DNA%20to%20OmpA%20constructs>Cloning of protein A DNA to OmpA constructs </a></li>
<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=5&arg0=21_July_2008&arg1=22_July_2008&arg2=23_July_2008&arg3=24_July_2008&arg4=25_July_2008&name=Cloning%20of%20protein%20A%20DNA%20to%20OmpA%20constructs>Cloning of protein A DNA to OmpA constructs </a></li>
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=3&arg0=29_July_2008&arg1=30_July_2008&arg2=31_July_2008&name=Cloning%20of%20omega_%26Delta%3BA%20DNA%20fragment%20to%20pACYC177%20OmpA_alpha>Cloning of omega_&Delta;A DNA fragment to pACYC177 OmpA_alpha </a></li>
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=5&arg0=30_July_2008&arg1=31_July_2008&arg2=1_August_2008&arg3=4_August_2008&arg4=5_August_2008&name=Cloning%20of%20truncated%20fragment%20of%20protein%20A%20%28%26Delta%3BA%29>Cloning of truncated fragment of protein A (&Delta;A) </a></li>
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=5&arg0=18_August_2008&arg1=19_August_2008&arg2=20_August_2008&arg3=21_August_2008&arg4=22_August_2008&name=Cloning%20of%20protein%20A%20DNA%20to%20GeneArt%20plasmid>Cloning of protein A DNA to GeneArt plasmid </a></li>
<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=5&arg0=18_August_2008&arg1=19_August_2008&arg2=20_August_2008&arg3=21_August_2008&arg4=22_August_2008&name=Cloning%20of%20protein%20A%20DNA%20to%20GeneArt%20plasmid>Cloning of protein A DNA to GeneArt plasmid </a></li>
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<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=4&amp;arg0=4_August_2008&amp;arg1=5_August_2008&amp;arg2=6_August_2008&amp;arg3=7_August_2008&amp;name=Preparing%20pACYC177%20OmpA_omega_%26Delta%3BA%20construct">Preparing pACYC177 OmpA_omega_&Delta;A construct</a>
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<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=5&amp;arg0=18_August_2008&amp;arg1=19_August_2008&amp;arg2=20_August_2008&amp;arg3=21_August_2008&amp;arg4=22_August_2008&amp;name=Cloning%20of%20protein%20A%20DNA%20to%20GeneArt%20plasmid">Cloning of protein A DNA to GeneArt plasmid</a></li>
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=5&amp;arg0=18_August_2008&amp;arg1=19_August_2008&amp;arg2=20_August_2008&amp;arg3=21_August_2008&amp;arg4=22_August_2008&amp;name=Cloning%20of%20protein%20A%20DNA%20to%20GeneArt%20plasmid">Cloning of protein A DNA to GeneArt plasmid</a></li>
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<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=2&amp;arg0=24_September_2008&amp;arg1=25_September_2008&amp;name=Preparation%20of%20alpha_A%20construct">Preparation of alpha_A construct</a></li>
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=2&amp;arg0=24_September_2008&amp;arg1=25_September_2008&amp;name=Preparation%20of%20alpha_A%20construct">Preparation of alpha_A construct</a></li>
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=4&arg0=4_August_2008&arg1=5_August_2008&arg2=6_August_2008&arg3=7_August_2008&name=Preparing%20pACYC177%20OmpA_omega_%26Delta%3BA%20construct>Preparing pACYC177 OmpA_omega_&Delta;A construct </a></li>
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<li class="nb_toc1"><a OnClick=switchMenu('delta_A','submenu_item')>Preparation of vectors with B-domain of protein A derivatives fusions</a></li>
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=3&arg0=29_July_2008&arg1=30_July_2008&arg2=31_July_2008&name=Cloning%20of%20omega_%26Delta%3BA%20DNA%20fragment%20to%20pACYC177%20OmpA_alpha>Cloning of omega_&Delta;A DNA fragment to pACYC177 OmpA_alpha </a></li>
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=5&arg0=30_July_2008&arg1=31_July_2008&arg2=1_August_2008&arg3=4_August_2008&arg4=5_August_2008&name=Cloning%20of%20truncated%20fragment%20of%20protein%20A%20%28%26Delta%3BA%29>Cloning of truncated fragment of protein A (&Delta;A) </a></li>
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<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=4&arg0=4_August_2008&arg1=5_August_2008&arg2=6_August_2008&arg3=7_August_2008&name=Preparing%20pACYC177%20OmpA_omega_%26Delta%3BA%20construct>Preparing pACYC177 OmpA_omega_&Delta;A construct </a></li>
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<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=4&amp;arg0=4_August_2008&amp;arg1=5_August_2008&amp;arg2=6_August_2008&amp;arg3=7_August_2008&amp;name=Preparing%20pACYC177%20OmpA_omega_%26Delta%3BA%20construct">Preparing pACYC177 OmpA_omega_&Delta;A construct</a>
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<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=9&amp;arg0=13_August_2008&amp;arg1=14_August_2008&amp;arg2=18_August_2008&amp;arg3=19_August_2008&amp;arg4=20_August_2008&amp;arg5=21_August_2008&amp;arg6=22_August_2008&amp;arg7=25_August_2008&amp;arg8=26_August_2008&amp;name=Cloning%20of%20alpha%20to%20pACYC177%20OmpA_A_omega%20and%20pACYC177%20OmpA_Z_omega">Cloning of alpha to pACYC177 OmpA_A_omega and pACYC177 OmpA_Z_omega</a></li>
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=9&amp;arg0=13_August_2008&amp;arg1=14_August_2008&amp;arg2=18_August_2008&amp;arg3=19_August_2008&amp;arg4=20_August_2008&amp;arg5=21_August_2008&amp;arg6=22_August_2008&amp;arg7=25_August_2008&amp;arg8=26_August_2008&amp;name=Cloning%20of%20alpha%20to%20pACYC177%20OmpA_A_omega%20and%20pACYC177%20OmpA_Z_omega">Cloning of alpha to pACYC177 OmpA_A_omega and pACYC177 OmpA_Z_omega</a></li>

Revision as of 12:44, 29 October 2008

Gallery Bricks Notebook Team Project Home

Table of contents

 

Preparation of one-armed bandit vectors

One-armed bandit tests

Preparation of vectors for 'hunter' part of 'hunter and prey'

Preparation of vectors for 'prey' part of 'hunter and prey'

Purification of prey proteins

'Hunter and prey' tests

Preparation of BioBricks

Other activities

May
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
June
MTWTFSS
            1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30
July
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30 31
August
MTWTFSS
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
September
MTWTFSS
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30
October
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
November
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30

Protocols

Primers

Vectors

JavaScript tools developed to make working with iGEM notebook more comfortable

Strains

Rosetta(DE3)pLysS

F- ompT hsdSB(RB- mB-) gal dcm λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5]) pLysSRARE (CamR)

TOP10 (Invitrogen)

F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ(ara-leu)7697 galE15 galK16 rpsL(StrR) endA1 λ-

NR9458 (IBB collection; in notebook called mutD5)

ara thi Δ pro lac mutD5 zaf-13 ::Tn10

GM2163

ara-14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm-6 hisG4 rfbD1 R(zgb210::Tn10)TetS endA1 rpsL136 dam13::Tn9 xylA-5 mtl-1 thi-1 mcrB1 hsdR2