"
TUDelft/7 October 2008
From 2008.igem.org
(Difference between revisions)
(→Sonication optimalization) |
|||
| Line 5: | Line 5: | ||
=October 7th= | =October 7th= | ||
| - | ==Sonication | + | ==Sonication optimization== |
| + | To optimize the new use of sonication for lysing cells, we'll be trying the effect of different lengths of sonication on our protein content and luciferase activity. We'll be trying a series of: | ||
| + | * 1 to 5 times 15 seconds sonication, with 15 seconds pause. | ||
| + | * 1 to 4 times 30 seconds sonication, with 30 seconds pause. | ||
| + | * 1 times 60 seconds sonication. | ||
| + | Because earlier luciferase measurements weren't done in duplo, this time we've tried this to see whether it's necessary for following experiments. | ||
| + | Results are displayed in figure 1. | ||
| + | |||
| + | [[Image:TUDelft071008.jpg|Figure 1]] | ||
{{Template:TUDelftiGEM2008_sidebar}} | {{Template:TUDelftiGEM2008_sidebar}} | ||
Revision as of 11:47, 20 October 2008
| July | ||||||
| M | T | W | T | F | S | S |
| 1 | 2 | 3 | 4 | 5 | 6 | |
| 7 | 8 | 9 | 10 | 11 | 12 | 13 |
| 14 | 15 | 16 | 17 | 18 | 19 | 20 |
| 21 | 22 | 23 | 24 | 25 | 26 | 27 |
| 28 | 29 | 30 | 31 | |||
| August | ||||||
| M | T | W | T | F | S | S |
| 1 | 2 | 3 | ||||
| 4 | 5 | 6 | 7 | 8 | 9 | 10 |
| 11 | 12 | 13 | 14 | 15 | 16 | 17 |
| 18 | 19 | 20 | 21 | 22 | 23 | 24 |
| 25 | 26 | 27 | 28 | 29 | 30 | 31 |
| September | ||||||
| M | T | W | T | F | S | S |
| 1 | 2 | 3 | 4 | 5 | 6 | 7 |
| 8 | 9 | 10 | 11 | 12 | 13 | 14 |
| 15 | 16 | 17 | 18 | 19 | 20 | 21 |
| 22 | 23 | 24 | 25 | 26 | 27 | 28 |
| 29 | 30 | |||||
| October | ||||||
| M | T | W | T | F | S | S |
| 1 | 2 | 3 | 4 | 5 | ||
| 6 | 7 | 8 | 9 | 10 | 11 | 12 |
| 13 | 14 | 15 | 16 | 17 | 18 | 19 |
| 20 | 21 | 22 | 23 | 24 | 25 | 26 |
| 27 | 28 | 29 | 30 | 31 | ||
October 7th
Sonication optimization
To optimize the new use of sonication for lysing cells, we'll be trying the effect of different lengths of sonication on our protein content and luciferase activity. We'll be trying a series of:
- 1 to 5 times 15 seconds sonication, with 15 seconds pause.
- 1 to 4 times 30 seconds sonication, with 30 seconds pause.
- 1 times 60 seconds sonication.
Because earlier luciferase measurements weren't done in duplo, this time we've tried this to see whether it's necessary for following experiments.
Results are displayed in figure 1.
