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  • Team:Chiba/jk/β/weaks 1-2
    :'''[[Team:Chiba/protocol/transformation|Transformation]]''' :'''[[Team:Chiba/protocol/PCR|PCR]]'''
    43 KB (6,909 words) - 07:07, 20 September 2008
  • Team:Chiba/jk/β/week 2
    >[[Team:Chiba/protocol/j|Protocols]] :(22/8)--->'''[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]'''
    22 KB (3,468 words) - 12:11, 23 October 2008
  • Team:Chiba/notebook/input/august
    '''[[Team:Chiba/protocol/PCR|PCR]]''' -->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
    17 KB (2,289 words) - 05:02, 24 October 2008
  • Team:Chiba/jk/β/week 3
    >[[Team:Chiba/protocol/j|Protocols]] :'''[[Team:Chiba/protocol/transformation|Transformation]]'''
    26 KB (4,447 words) - 04:05, 24 October 2008
  • Team:Chiba/Calendar-Home/30 August 2008
    '''[[Team:Chiba/protocol/digestion|Digestion]]''' -->30μl'''[[Team:Chiba/protocol/ligation/gelex|Gel extraction]]'''
    11 KB (1,784 words) - 23:13, 29 October 2008
  • Team:Chiba/protocol/j
    ===[[Team:Chiba/protocol/transformation/j|Z コンピ]] === ===[[Team:Chiba/protocol/DNA_Purification/sigma/j|シグマプレップ]]===
    4 KB (188 words) - 05:02, 27 June 2009
  • Team:Cambridge/Bacillus/Lab Work
    '''Protocol''' *Add Disks in antibiotic solution for 5 mins [ Protocol to sterilize tweezers : Wipe with Kimwipes, Ethanol, Flame ]
    65 KB (9,746 words) - 03:56, 30 October 2008
  • Team:Chiba/protocol
    ===[[Team:Chiba/protocol/transformation|Zymo comp.]] === ===[[Team:Chiba/protocol/transformation|Electro poration]]===
    1 KB (181 words) - 12:46, 16 December 2008
  • Team:iHKU/protocol
    <td><a href="/Team:iHKU/protocol"><img src="/wiki/images/f/f2/Button_protocol.jpg" width="200" height="25" / <li> PROTOCOL-1 <a href="#p1">Pattern formation</a></li>
    63 KB (6,466 words) - 19:25, 29 October 2008
  • Team:Chiba/jk/β/week 1
    >[[Team:Chiba/protocol/j|Protocols]] :'''[[Team:Chiba/protocol/transformation|Transformation]]'''
    22 KB (3,633 words) - 12:34, 30 September 2008
  • Team:Chiba/jk/β/week 5
    >[[Team:Chiba/protocol/j|Protocols]] :(13/9)--->'''[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]'''
    6 KB (804 words) - 13:20, 1 October 2008
  • GenProtocols
    This protocol is designed for the purification of up to 20 ug high-copy plasmid DNA from ==Ligation protocol, using Roche Rapid Ligation Kit==
    17 KB (2,715 words) - 19:24, 26 October 2008
  • Team:BrownTwo/Notebook
    ...or about 4.5 hours since other obligations prevented us from finishing the protocol. Normally, we would have plated the transformations immediately after they <p>Gel Mixture Protocol for 1% Agarose gel:</p>
    226 KB (32,672 words) - 04:53, 29 October 2008
  • Team:Paris/August 8
    ==== '''Protocol''' ==== ==== Protocol : ====
    18 KB (2,438 words) - 13:22, 15 August 2008
  • Team:Chiba/jk/β/weaks 3-4
    :'''[[Team:Chiba/protocol/transformation|Transformation]]''' '''[[Team:Chiba/protocol/digestion|Digestion]]'''
    13 KB (2,259 words) - 07:03, 20 September 2008
  • Team:Harvard/GenProtocols
    ==Ligation protocol, using Roche Rapid Ligation Kit== ===Brief Protocol===
    13 KB (2,102 words) - 05:38, 29 October 2008
  • Team:Paris/August 15
    ==='''Protocol'''=== ...ly competent cells [[Team:Paris/Notebook/Protocols#Transformation|standard protocol]].
    9 KB (1,174 words) - 18:06, 4 September 2008
  • Team:Chiba/Calendar-Home/1 September 2008
    '''[[Team:Chiba/protocol/gelcheck|Gel Check]]''' '''[[Team:Chiba/protocol/transformation|Transformation]]'''(Double Transformation)
    8 KB (1,254 words) - 01:42, 30 October 2008
  • Team:Chiba/jk/γ/week 2
    [[Team:Chiba/protocol/transformation|Transformation]] [[Team:Chiba/protocol/digestion|Digestion]]
    9 KB (1,396 words) - 05:50, 24 October 2008
  • Team:Chiba/Calendar-Home/26 August 2008
    :(8/25)--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]''' --->'''[[Team:Chiba/protocol/ligation/gelex|Gel Extract]]'''
    7 KB (1,128 words) - 01:04, 30 October 2008
  • Team:Calgary Wetware/Protocols
    ...I wrote up a procedure for diluting primers. As an added bonus, contains a protocol for PCR purification! [[Team:Calgary_Wetware/Protocols/Diluting|Check it ou <li><a href="#transformation" title="Bacterial Transformation Protocol"> Bacterial Transformation </a></li>
    23 KB (3,681 words) - 23:18, 29 October 2008
  • Team:TUDelft/Protocols
    ...We did make a stock of chemically competent DB3.1 cells with the following protocol (found on OpenWetWare). We found that these cells were indeed very competen ''Protocol found on OpenWetWare''
    19 KB (3,279 words) - 23:04, 29 October 2008
  • Team:KULeuven/Data/Other Parts
    ...s can be tested (the construct that have to be made and the actual testing protocol). It will also give an overview of the results we had so far. We worked as === Construct & protocol ===
    14 KB (2,307 words) - 23:28, 29 October 2008
  • Team:Chiba/jk/β/week 4
    [[Team:Chiba/protocol/digestion|Digestion Test]] :--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
    5 KB (714 words) - 12:52, 30 September 2008
  • Team:Paris/August 6
    ==== Protocol ==== ====Protocol====
    8 KB (1,222 words) - 14:06, 13 August 2008
  • Team:Illinois/Bimolecular Fluorescence Biosensor Notebook
    ** PCR protocol as in paper for both N terminal * Gel Extraction Protocol
    14 KB (2,108 words) - 18:50, 29 October 2008
  • Team:Chiba/Calendar-Home/28 August 2008
    '''[[Team:Chiba/protocol/transformation|Transformation]]''' '''[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]'''
    5 KB (757 words) - 03:14, 30 October 2008
  • July
    -&gt; protocol 07-10-2008 <br> we repeated step 5 and 6 of the protocol.<br>
    22 KB (3,483 words) - 02:40, 30 October 2008
  • Team:Imperial College/Light Inducible
    The aim of this protocol is to characterise the light induced expression of GFPmut3b under the contr ======Protocol======
    6 KB (938 words) - 17:44, 28 October 2008
  • Team:Hawaii/Make Competent E. Coli
    ...at6709852.pdf | Bloom04]] patent describes the use of essentially the same protocol for the Invitrogen Mach 1 cells. '''This is the chemical transformation protocol used by [[User:Tk|Tom Knight]] and the [http://partsregistry.org Registry o
    7 KB (1,158 words) - 23:54, 30 May 2008
  • Team:University of Lethbridge/Notebook/Project1August
    ...ok & Russell, (2001) ''Molecular Cloning: A Laboratory Manual 3rd Edition. Protocol 25'' '''Vol. 1; 1.117'''): Protocol:
    13 KB (1,866 words) - 02:06, 30 October 2008
  • Team:Paris/August 27
    [[Team:Paris/Notebook/Protocols#Concentration_of_the_Miniprep | Protocol (it's same that for Miniprep)]] [[Team:Paris/Notebook/Protocols#Digestion|Protocol Digestion]]
    5 KB (688 words) - 19:16, 9 September 2008
  • Team:KULeuven/Data/Other Components
    ... can be tested (the constructs that have to be made and the actual testing protocol). It will also give an overview of the results we had so far. We worked as === Construct & protocol ===
    9 KB (1,463 words) - 20:13, 29 October 2008
  • Team:Hawaii/Initial PCC6803 Transformation
    == Protocol by Arizona State University == == Protocol by Shao, ''et al'' ==
    4 KB (628 words) - 06:54, 27 June 2008
  • Team:Hawaii/Initial E. Coli Transformation
    == Protocol by Invitrogen == == Protocol from SC Lab ==
    6 KB (893 words) - 02:31, 2 August 2008
  • Team:Paris/August 7
    *Concerning '''pflhB, pflgA and pflgB''', the protocol seems to be '''very operational''': we always have great results ! *Standart protocol except the last step. Instead of eluting with water, we used 30 µL of BE b
    10 KB (1,412 words) - 12:42, 13 August 2008
  • Team:Illinois/Antibody GPCR Fusion Notebook
    **Protocol from Wiley's ''Current Protocols in Molecular Biology'' ** Result: Failed to finish protocol
    13 KB (1,969 words) - 02:55, 30 October 2008
  • Team:Paris/August 22
    [[Team:Paris/Notebook/Protocols#Digestion|Protocol Digestion]] [[Team:Paris/Notebook/Protocols#Migration after extraction |Protocol]]
    7 KB (891 words) - 00:54, 21 September 2008
  • Team:Chiba/Calendar-Home/29 August 2008
    :--->'''[[Team:Chiba/protocol/digestion|Digestion Test]]''' :--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
    5 KB (803 words) - 20:28, 29 October 2008
  • Team:Paris/August 21
    ==Protocol== [[Team:Paris/Notebook/Protocols#Digestion|Protocol Digestion]]
    7 KB (836 words) - 14:29, 6 September 2008
  • Team:Paris/August 26
    ==Protocol== '''Protocol'''
    11 KB (1,582 words) - 17:59, 11 September 2008
  • Team:Chiba/jk/γ/week 3
    [[Team:Chiba/protocol/digestion|Digestion]] [[Team:Chiba/protocol/ligation/ligation|Ligation]]
    6 KB (946 words) - 06:30, 24 October 2008
  • Team:Chiba/jk/γ/week 4
    [[Team:Chiba/protocol/digestion|Digestion]] -->[[Team:Chiba/protocol/ligation/gelex|Gel Extraction]]
    6 KB (907 words) - 06:52, 24 October 2008
  • Team:TUDelft/Temperature results
    ...ure_results#Setting_up_luciferase_measurements|Obtaining a correct working protocol to measure luminescence]] ...onstruction of all devices the 3A assembly strategy was chosen. A complete protocol of this strategy can be found on [http://openwetware.org/wiki/Synthetic_Bio
    21 KB (3,339 words) - 10:56, 30 October 2008
  • Team:Chiba/Calendar-Home/19 September 2008
    '''[[Team:Chiba/protocol/gelcheck|Gel Check]]''' --->'''[[Team:Chiba/protocol/ligation/gelex|Gel Extract]]'''<br>
    3 KB (476 words) - 00:20, 30 October 2008
  • Team:Imperial College/Transformation Results
    ...m:Imperial_College/Transformation Click this link for full Transformation protocol 1 '''] '''Brief Description of Protocol'''<br>
    4 KB (559 words) - 01:59, 30 October 2008
  • Team:Michigan/Notebook/PCRProtocol
    == <font color=royalblue size=5> PCR Standard Protocol </font> == ... machines may also require different mixtures to run properly. This is the protocol specified for our machine, the PCR Express.
    2 KB (365 words) - 03:18, 30 October 2008
  • Team:Cambridge/Signalling/Lab Work
    * found protocol for Bacillus subtilis transformation ** Miniprep I746001 and I746101 pellets (plasmid mniprep kit) : same protocol but 60μL of Zyppy Glution Buffer in step 8
    36 KB (5,743 words) - 04:15, 30 October 2008
  • Extract DNA (Maxiprep or Midiprep)
    ==Midiprep/ Maxiprep Growth Protocol== ==Midiprep Protocol (QIAGEN kit)==
    6 KB (1,068 words) - 01:00, 30 October 2008
  • Team:Hawaii/Initial Synth. Oligo Assembly
    ==Protocol== ...e.org/wiki/Engineering_BioBrick_vectors_from_BioBrick_parts/Colony_PCR her protocol on OpenWetWare]. No visible difference was detected between the three annea
    19 KB (3,019 words) - 16:40, 25 July 2008
  • Team:Beijing Normal/Project
    == Special protocol == ...ng to complete failure. To solve this problem, we have developed a special protocol-- hot start method combined with additive(sole or mixed)-- which is most he
    18 KB (2,612 words) - 04:27, 30 October 2008
  • Team:Paris/August 20
    *[[Team:Paris/Notebook/Protocols#Glycerol stocks| Protocol stocks]] *[[Team:Paris/Notebook/Protocols#Minipreps (Kit_Qiagen)| Protocol Miniprep]]
    9 KB (1,183 words) - 14:03, 6 September 2008
  • Team:Chiba/Calendar-Home/20 August 2008
    '''[[Team:Chiba/protocol/PCR|PCR]]''' -->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
    8 KB (1,334 words) - 05:57, 30 October 2008
  • Team:Chiba/Calendar-Home/25 August 2008
    :(24/8)--->'''[[Team:Chiba/protocol/digestion|Digestion]]''' :--->[[Team:Chiba/protocol/gelcheck|Gel Check]]
    7 KB (1,106 words) - 06:15, 30 October 2008
  • Team:Chiba/Calendar-Home/27 August 2008
    [[Team:Chiba/protocol/digestion|Digestion Test]] :--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
    4 KB (608 words) - 20:14, 29 October 2008
  • Team:Harvard/Dailybook/Week3/Chemical and Light
    ... 2008 registries. We made the following changes to the iGEM transformation protocol: After the gel extraction (standard kit protocol), the negative control (gel with no DNA) read 5.4 ng/μL and the positive c
    30 KB (4,485 words) - 19:02, 26 October 2008
  • Team:Bologna/UV radiation/
    ==Protocol used for UV induction== ...id with our construct has been transformed in DH5alfa bacteria by standard protocol and one colony from the plate has been picked and cultured overnight in 5m
    12 KB (2,040 words) - 09:00, 29 October 2008
  • DNA restriction digest
    List reagents, supplies and equipment necessary to perform the protocol here. For those materials which have their own OWW pages, link to that pag <!-- If this protocol has papers or books associated with it, list those references here. -->
    4 KB (515 words) - 01:22, 17 July 2008
  • Team:ETH Zurich/Wet Lab/Protocol
    ...<td><table><tr><td width=5px></td><td>[[Team:ETH_Zurich/Wet%20Lab/Protocol|Protocol]]</td></tr><tr><td width=5px></td><td><table></table></td></tr></table></td ...><span lang="en-US" id="d8on110">gel extraction was performed according to protocol:</span></p>
    68 KB (10,408 words) - 13:17, 18 August 2008
  • Team:EPF-Lausanne/Notebook
    We followed the protocol coming from [http://openwetware.org/wiki/TOP10_chemically_competent_cells O ...alculations, follow [http://openwetware.org/wiki/DNA_ligation DNA Ligation Protocol on OWW]
    16 KB (2,322 words) - 23:27, 29 October 2008
  • Team:Paris/August 1
    * Use of Quiagen's protocol on all the clones cultivated on the 31th. === Protocol ===
    10 KB (1,668 words) - 16:35, 23 August 2008
  • Team:Chiba/Calendar-Home/31 August 2008
    :'''[[Team:Chiba/protocol/transformation|Transformation]]''' :'''[[Team:Chiba/protocol/digestion|Digestion]]'''
    5 KB (713 words) - 20:41, 29 October 2008
  • Imperial College/17 August 2008
    *Prepare media and reagents for the protocol transformation 1 protocol *Prepare competent cells using the transformation 2 protocol, this time growing cells to an o.D.<sub>600</sub>
    3 KB (348 words) - 20:37, 28 October 2008
  • Team:Heidelberg/Notebook/team meetings
    ... || [[Image:text-icon.png|20px]] [[Media:protocol_20081021.txt | Protocol]]|| [[Image:icon-ppt.png|20px]] Report Phages | || [[Image:pdf-logo.jpg|20px]] [[Media:protocol_20081014.pdf | Protocol]]
    24 KB (3,294 words) - 17:13, 29 October 2008
  • Team:Chiba/Calendar-Home/2 September 2008
    :(31/8)--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]''' :(1/9)--->'''[[Team:Chiba/protocol/PCR| Colony PCR]]'''
    6 KB (905 words) - 03:10, 30 October 2008
  • Team:Chiba/Calendar-Home/14 September 2008
    (13/9)--->'''[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]''' (13/9)--->[[https://2008.igem.org/Team:Chiba/protocol/ligation/dephosphorylation|SAP]]
    3 KB (440 words) - 23:59, 29 October 2008
  • Team:Imperial College/Constitutive
    *To characterise the constitutive promoter and RBS combinations. This protocol is concerned with characterisation of the steady-state expression of GFPmut *The protocol below describes what is needed for one combination of promoter and RBS, we
    3 KB (559 words) - 17:43, 28 October 2008
  • Team:Imperial College/Inducible
    *The aim of this protocol is to help us define the dynamic range of the transfer function where the l *This protocol is to be used to characterise the IPTG inducible Hyper-spank promoter RBS c
    3 KB (495 words) - 17:39, 28 October 2008
  • Team:Harvard/Dailybook/Week0
    *Miniprep vectors ([[Team:Harvard/GenProtocols#QIAprep_Spin_Miniprep_Kit|protocol]]) ...d/GenProtocols#Chemically_competent_cells|protocol]]) - actually used Zymo protocol which didn't work for Shewie and I don't think for E. coli
    17 KB (1,690 words) - 17:34, 28 October 2008
  • Team:Hawaii/Protocols/Competent Cells
    ...at6709852.pdf | Bloom04]] patent describes the use of essentially the same protocol for the Invitrogen Mach 1 cells. '''This is the chemical transformation protocol used by [[User:Tk|Tom Knight]] and the [http://partsregistry.org Registry o
    5 KB (802 words) - 04:22, 24 June 2008
  • Team:MIT
    ...:MIT/Transforming_Lactococcus lactis|''Lactococcus lactis'' Transformation Protocol]] ...L._acidophilus_transformation ''Lactobacillus acidophilus'' Transformation Protocol]
    7 KB (996 words) - 09:46, 30 October 2008
  • Team:Hawaii/Large-Scale Preparation of Plasmid from E. coli
    ...can be obtained by using CsCl/ethidium bromide equilibrium centrifugation (protocol found in Maniatis). #*The protocol recommends using 6,000 x g.
    10 KB (1,527 words) - 08:31, 10 July 2008
  • Team:University of Lethbridge/Notebook/GeneralLabJuly
    Protocol changes: ...n from the filter paper. Next week we will attempt various changes to the protocol to extract more DNA.
    6 KB (838 words) - 02:29, 30 October 2008
  • Team:Paris/July 28
    === Protocol === === PCR Protocol ===
    20 KB (2,506 words) - 16:59, 13 August 2008
  • Team:Paris/July 29
    === Protocol === === Protocol ===
    9 KB (1,357 words) - 17:01, 13 August 2008
  • Team:Paris/August 12
    ====Protocol==== ...was two fragments, the standard [[Team:Paris/Notebook/Protocols#Extraction|protocol]]
    11 KB (1,508 words) - 11:00, 15 August 2008
  • Team:Paris/August 16
    ...he [[Team:Paris/Notebook/Protocols#Purification_.28Kit_Promega.29|standard protocol.]] ...ed the DNA following the [[Team:Paris/Notebook/Protocols#Ligation|standard protocol]].
    5 KB (652 words) - 18:08, 4 September 2008
  • Team:Paris/August 18
    *[[Team:Paris/Notebook/Protocols#Glycerol stocks| Protocol stocks]] *[[Team:Paris/Notebook/Protocols#Minipreps (Kit_Qiagen)| Protocol minprep]]
    5 KB (622 words) - 19:50, 4 September 2008
  • Imperial College/2 September 2008
    ...://openwetware.org/index.php?title=IGEM:IMPERIAL/2008/Prototype/Wetlab/PCR protocol], using the optimal conditions determined yesterday and results run on a ge *The results of this protocol are shown on figure 1 a 1% agarose gel. The lanes concerned are 1-4 and rep
    4 KB (654 words) - 20:43, 28 October 2008
  • Team:University of Lethbridge/Notebook/Project1October
    ...ation of BL21 and RP1616 with pUC19 (positive control) and pTopp using the protocol Jaden and Selina used on August 25, 2008. Protocol (from Kothe Lab):
    10 KB (1,545 words) - 02:01, 30 October 2008
  • Team:Chiba/Calendar-Home/22 August 2008
    '''[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]''': insert check:'''[[Team:Chiba/protocol/digestion|Digestion]]'''
    4 KB (576 words) - 03:02, 30 October 2008
  • Team:Chiba/jk/γ/week 5
    [[Team:Chiba/protocol/gelcheck|Gel check]] [[Team:Chiba/protocol/gelcheck|Gel check]]
    7 KB (1,028 words) - 07:14, 24 October 2008
  • Team:Chiba/Calendar-Home/3 September 2008
    :(2/9)--->'''[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]''' :--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
    7 KB (1,339 words) - 21:03, 29 October 2008
  • Team:Chiba/Calendar-Home/8 September 2008
    '''[[Team:Chiba/protocol/ligation/gelex|Gel Extract]]''' :--->'''[[Team:Chiba/protocol/DNA Purification/zymocleam|Zymo Clean]]'''
    4 KB (631 words) - 23:43, 29 October 2008
  • Team:Chiba/Calendar-Home/9 September 2008
    (6/9)--->[[Team:Chiba/protocol/PCR|PCR]] of [http://partsregistry.org/Part:BBa_I9030 BBa_I9030] --->'''[[Team:Chiba/protocol/ligation/gelex|Gel Extract]]'''
    3 KB (546 words) - 01:26, 30 October 2008
  • Team:Chiba/Calendar-Home/10 September 2008
    '''[[Team:Chiba/protocol/PCR|Colony-PCR]]''' -->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
    3 KB (476 words) - 23:10, 29 October 2008
  • Team:Chiba/Calendar-Home/18 September 2008
    *[[Team:Chiba/protocol/phenotype/timedelay/j|time-delay test]] [[Team:Chiba/protocol/ligation/dephosphorylation|SAP]] 1&mu;L
    3 KB (380 words) - 00:01, 30 October 2008
  • Team:Chiba/Calendar-Home/25 September 2008
    <br>colony [[Team:Chiba/protocol/PCR|PCR]] <br>[[Team:Chiba/protocol/digestion|Digestion test]]
    4 KB (667 words) - 23:29, 29 October 2008
  • Team:Heidelberg/Notebook/Killing I/Notebook/week9
    PCR protocol PCR protocol
    33 KB (4,755 words) - 11:45, 29 October 2008
  • Team:NYMU-Taipei/Project/Phosphate/Experiments/GEL Electrophoresis
    ! Protocol ! Protocol
    27 KB (3,602 words) - 20:00, 29 October 2008
  • Team:Michigan/Notebook/GelProtocol
    == <font color=royalblue size=5> Analytical Gel Standard Protocol </font> == == <font color=royalblue size=5> Gel Extraction Standard Protocol </font> ==
    1 KB (189 words) - 03:20, 30 October 2008
  • Run Gel/ Gel extraction
    ==Run Gel Protocol== ==Gel Extraction Protocol using QIAquick Gel Extraction Kit:==
    3 KB (453 words) - 00:56, 30 October 2008
  • Team:Illinois/Antibody GPCR Fusion
    Protocol for Homologous Recombination [http://www.mrw.interscience.wiley.com/emrw/97 - We can use Basic Protocol 2 or 3
    21 KB (3,227 words) - 02:19, 24 October 2008
  • Imperial College/3 September 2008
    ...n 60°C for 20 cycles. Aad9 PCR was again tested according to the standard protocol and agin at 52°C for the first 10 cycles then 58°C or 56°C for 20 cycles ...k firstly if the primers are working and secondly if the single colony PCR protocol is working we carried out a series of testing. First to test the verificat
    7 KB (1,168 words) - 20:44, 28 October 2008
  • Team:Hawaii/Protocols/Plasmid prep
    == Miniprep Protocol== #*The protocol recommends using 6,000 x g.
    5 KB (806 words) - 04:22, 1 September 2008
  • EPF-Lausanne/16 July 2008
    Try new transformation with some iGEM part which we don't need and adjust protocol. We need to redo the transformation to troubleshoot. Perhaps the protocol which we were using was not the right one, so we modify it according to the
    2 KB (422 words) - 12:14, 7 August 2008
  • Team:ETH Zurich/Wet Lab/Protocoll
    ...><span lang="en-US" id="d8on110">gel extraction was performed according to protocol:</span></p> ...bottom: 0in;"><span lang="en-US" id="yxzy4">repitition of PCR with changed protocol</span></div>
    68 KB (10,408 words) - 14:52, 3 August 2008
  • Team:Paris/July 25
    * Use of Promega's protocol for the extraction of: gel n°1 --> 4 : (D100 --> D110 cl-1 ES)) * Use of Qiagen's protocol for the extraction of: gel n°5 --> 8 : (D110 cl-2 ES --> D120))
    2 KB (334 words) - 15:32, 31 July 2008
  • Team:Bologna/Notebook
    ...he new [http://partsregistry.org/Measurement Biobrick Standard Measurement Protocol] * Protocol design for isolation of single operators from the library.
    15 KB (2,315 words) - 01:13, 30 October 2008
  • Team:Imperial College/Protocols
    *[[Team:Imperial College/Transformation |Protocol 1]] *[[Team:Imperial College/Transformation_2 |Protocol 2]]
    2 KB (199 words) - 21:14, 29 October 2008
  • EPF-Lausanne/30 July 2008
    ==2 Step PCR Protocol== ... around the edges. They were left to bake overnight at 80°C following the protocol.
    4 KB (592 words) - 12:12, 7 August 2008
  • Team:Chiba/Calendar-Home/18 August 2008
    '''[[Team:Chiba/protocol/transformation|Transformation]]''' :'''[[Team:Chiba/protocol/PCR|PCR]]'''
    3 KB (451 words) - 05:13, 30 October 2008
  • Imperial College/21 August 2008
    ...b/Transformation_protocol_4#Transformation_Protocol_2| Click link here for protocol]. However, colonies were seen on the plates were no DNA was used for transf ..._protocol_2| click here for a link to the protocol]. The principle of this protocol is that a culture is grown to a high O.D.<sub>600</sub>, this induces the s
    3 KB (439 words) - 20:38, 28 October 2008
  • Team:Chiba/Calendar-Home/21 August 2008
    :(20/8)--->'''[[Team:Chiba/protocol/PCR|PCR]]''' :--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
    4 KB (652 words) - 05:58, 30 October 2008
  • Wiki/Team:Warsaw/protocols
    ...ref="http://www.aabiot.com/pdf/protocols/dna_pur/plasmid/plasmid_mini.pdf">protocol</a> of producer.</p> ...a href="http://www.aabiot.com/pdf/protocols/dna_pur/fragments/gel_out.pdf">protocol</a> of producer.</p>
    17 KB (2,920 words) - 00:16, 30 October 2008
  • Team:Chiba/jk/β/weak 5
    :(13/9)--->'''[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]''' :'''[[Team:Chiba/protocol/transformation|Transformation]]'''
    2 KB (241 words) - 18:41, 24 September 2008
  • TUDelft/19 September 2008
    ===Protocol=== The protocol used in the case of most samples, when lysed by sonification was:<br>
    4 KB (550 words) - 11:23, 22 September 2008
  • Team:Chiba/jk/γ/week 1
    [[Team:Chiba/protocol/PCR|PCR]] [[Team:Chiba/protocol/transformation|Transformation]]
    3 KB (496 words) - 05:25, 24 October 2008
  • Team:Newcastle University/Bacillus subtilis
    ...bility in the ''B. subtilis'' culture prior to transformation. We used the protocol below to achieve this. ...s transient, and therefore observing correct timings in the transformation protocol is very important in increasing the population of competent cells.
    14 KB (1,803 words) - 21:35, 29 October 2008
  • Team:Chiba/Calendar-Home/23 August 2008
    :(21/8)--->'''[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]''' :--->'''[[Team:Chiba/protocol/digestion|Digestion test]]'''
    6 KB (927 words) - 06:02, 30 October 2008
  • Team:Chiba/Calendar-Home/24 August 2008
    :(22/8)--->'''[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]''' :--->'''[[Team:Chiba/protocol/digestion|Digestion test]]'''
    5 KB (838 words) - 06:07, 30 October 2008
  • Team:Chiba/Calendar-Home/4 September 2008
    :--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]''' :--->'''[[Team:Chiba/protocol/digestion|Digestion test]]'''
    4 KB (538 words) - 21:04, 29 October 2008
  • Team:Chiba/Calendar-Home/7 September 2008
    [[Team:Chiba/protocol/digestion|Digestion Test]] :--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
    4 KB (654 words) - 22:24, 29 October 2008
  • Team:Chiba/Calendar-Home/17 September 2008
    *[[Team:Chiba/protocol/phenotype/timedelay/j|time-delay test]] *[[Team:Chiba/protocol/phenotype/timedelay/j|time-delay test]]
    2 KB (342 words) - 22:35, 29 October 2008
  • Team:Chiba/Calendar-Home/20 September 2008
    --->(19/9)'''[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]] --->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
    2 KB (339 words) - 00:25, 30 October 2008
  • Team:Heidelberg/Notebook/Killing I/Notebook/week5
    ** protocol ** protocol see parameter measurement protocol
    31 KB (4,359 words) - 11:49, 29 October 2008
  • Team:Imperial College/PCR
    This protocol is desgined for use with the stratagene ''PfuUltra'' II Fusion DNA polymera A modified protocol for using Taq polymerase can be used if less fidelity is required, to obtai
    6 KB (1,016 words) - 01:27, 29 October 2008
  • Team:Harvard/Dailybook/Week5/Chemical and Light
    ... green dot on top) aliquots from 1L of culture. Done as per Jason's lab's protocol. ...o new dh5α competent cells w/ volumes of 1, 3, and 5uL, using Jason's new protocol. As a control transformed 1uL of pUC19.
    41 KB (5,434 words) - 19:03, 26 October 2008
  • Team:Harvard/Dailybook/Week6/Chemical and Light
    ...emove the PstI site from cph1 (P87A and B) according to the QuikChange kit protocol. We ran a PCR positive control and a transformation positive control. We tr We used the TOP10 transformation protocol for both cell types. We plated 125 μL of each sample on two plates.
    27 KB (4,294 words) - 19:18, 26 October 2008
  • Team:Heidelberg/Notebook/Killing II/2ndweek
    *Transformation of BBa_F2621 and BBa_F2622 with Registry protocol ...f BBa_T9002, BBa_F2621, BBa_F2622 and pColE9-J into MG1655 (Transformation protocol Chris)
    28 KB (3,873 words) - 21:21, 28 October 2008
  • EPF-Lausanne/29 September 2008
    #*PCR protocol: #*PCR protocol:
    2 KB (387 words) - 19:32, 28 October 2008
  • Team:Michigan/Notebook/DigestProtocol
    == <font color=royalblue size=5> Digest Standard Protocol </font> == =Digest Protocol=
    2 KB (337 words) - 03:03, 30 October 2008
  • Team:Bologna/Wetlab
    So, we decided to set a new protocol up for the isolation and cloning of single operator sites into standard Bio According to the protocol to test operator parts ([https://2008.igem.org/Team:Bologna/Modeling#Proced
    20 KB (3,124 words) - 12:57, 4 December 2008
  • Extract DNA (Miniprep)
    ==Miniprep Protocol (QIAGEN Miniprep kit)== '''Miniprep Growth Protocol'''
    4 KB (626 words) - 00:59, 30 October 2008
  • Team:Cambridge/Voltage/Lab Work
    ...th on the plates spread yesterday. There has been a few adjustments to the protocol. The results of yesterday's modified protocol still yielded no growth on the plates. Using yesterday's PCR product our ai
    21 KB (3,118 words) - 23:31, 29 October 2008
  • Team:University of Sheffield/Dimtry
    ===Original Protocol=== ...al protocol of CAI production, which is a slightly modified version of the protocol described in the paper,[1] was as follows:
    4 KB (530 words) - 13:15, 29 October 2008
  • Team:MIT/Experiments
    ...:MIT/Transforming_Lactococcus lactis|''Lactococcus lactis'' Transformation Protocol]] ...L._acidophilus_transformation ''Lactobacillus acidophilus'' Transformation Protocol]
    1 KB (153 words) - 01:22, 30 October 2008
  • Team:Tokyo Tech/Protocol
    !align="center" width=20% style="text-align:center"|[[Team:Tokyo_Tech/Protocol|Protcol]] == <font size=5>'''Protocol''' </font>==
    4 KB (554 words) - 04:42, 30 October 2008
  • Purdue/11 July 2008
    ... have 100% glycerol (sterile), so we're just going to follow the same iGEM protocol but with 100% glycerol *Dr. Clase also suggested a somewhat different protocol for making stocks, so we're going to do both side-by-side and see which is
    1 KB (168 words) - 17:31, 11 July 2008
  • Team:UNIPV-Pavia/Notebook/Week3
    ...lasmid digestion again for our 4 plasmids to open, following the digestion protocol (read Protocols section for details). ... well for BBa_B0030, whose fragment length is 15 bp, so we hoped that this protocol might actually work to open plasmids.
    11 KB (1,718 words) - 21:25, 26 October 2008
  • Team:Hawaii/Notebook/2008-06- 2
    :* Protocol suggests 23C but growth was too slow. Also, protocol is for Mach10 cells, which grows much faster. ... 6 seed stock tubes per [[Team:Hawaii/Make_Competent_E._Coli | compentency protocol]]
    3 KB (445 words) - 02:35, 5 June 2008
  • Team:TUDelft/Meetings
    ...ut the DNA in a gel, got unclear/weird images. It looks like with the IGEM protocol (includes a heating step up to 42oc), you loose DNA but many impurities und *'''PCR''': Ruud is setting up/checking available PCR protocol. 95-50-75 protocol with Taq polymerase seems expectedly working. ''Plans'': Put the genes into
    18 KB (2,754 words) - 09:07, 27 October 2008
  • Team:Hawaii/Biobricks extraction & transformation competency test
    == Protocol == The same protocol as [[Team:Hawaii/Initial_ BioBrick_Plasmid_Extraction_From_Filter_Paper|"In
    3 KB (433 words) - 02:40, 30 October 2008
  • Team:Hawaii/Notebook/2008-06-20
    ===Electroporation Protocol=== :* Posted electroporation protocol. It looks like this will be a viable option for introducing an autonomously
    705 B (98 words) - 06:51, 24 June 2008
  • Team:Heidelberg/Notebook/Killing I/Notebook/week10
    PCR protocol ...anted EcoRI restriction site in cI from geneart was mutated using standard protocol
    35 KB (4,900 words) - 14:09, 29 October 2008
  • Team:MIT/Tooth binding assay protocol
    A couple of things are novel in the protocol. Firstly, we modified the protocol bacteria on the beads (the paper with the original protocol failed to prove
    5 KB (780 words) - 21:12, 29 October 2008
  • Team:MIT/Protein Purification
    ===TEV Purification Protocol=== ===Peptide Purification Protocol===
    2 KB (298 words) - 21:08, 27 June 2008
  • Team:ESBS-Strasbourg/PCR
    -Adapt the protocol function of the mother concentration we have <br> b) Protocol <br>
    3 KB (472 words) - 12:44, 21 July 2008
  • Team:Tsinghua/Notebook
    '''1. Advanced Protocol for Parts Extraction''' We have revised the Parts Extraction Protocol and obtained a higher efficiency of transformation. Here are some details:
    10 KB (1,229 words) - 04:31, 30 October 2008
  • Team:KULeuven/16 July 2008
    ...9]) in competent TOP10 cells using the BioBrick protocol (and not the CMPG protocol). An empty pUC plasmid was used as control. Because the transformation didn ...s punched out and transformed into competent DH5alpha cells using the CMPG protocol, also with empty pUC control.
    1 KB (201 words) - 18:06, 6 October 2008
  • Team:Imperial College/Test Page
    <p class="num">Protocol 1</p> <p class="info">The actual protocol</p>
    5 KB (774 words) - 13:44, 8 September 2008
  • Team:University of Ottawa/6 August 2008
    :<li> Followed miniprep protocol included in kit ...d to be zero. Also, did not mix neutralisation solution as outlined in the protocol.
    5 KB (700 words) - 19:11, 7 August 2008
  • Team:Paris/August 11
    ...g [[Team:Paris/Notebook/Protocols#Transformation|transformation for Top10 protocol]] ==== '''Protocol''' ====
    8 KB (1,065 words) - 16:11, 18 August 2008
  • Team:Paris/August 13
    ...s://2008.igem.org/Team:Paris/Notebook/Protocols#Minipreps_.28Kit_Qiagen.29 Protocol] (see # '''3''') Experiments done by QIAcube * [https://2008.igem.org/Team:Paris/Notebook/Protocols#Glycerol_Stocks Protocol] (see #'''2''')
    8 KB (1,152 words) - 16:12, 15 August 2008
  • Team:Paris/August 19
    [[Team:Paris/Notebook/Protocols#Ligation |Protocol]] [[Team:Paris/Notebook/Protocols#Transformation |Protocol]]
    5 KB (652 words) - 20:11, 4 September 2008
  • Imperial College/31 August 2008
    *Begin to try to optimise the protocol for ''B.subtilis'' transformation protocol with linear DNA. This includes the volume of transformed cells, amount of D *Carry on to optimise the protocol for ''B.subtilis'' transformation protocol with linear DNA
    3 KB (359 words) - 20:42, 28 October 2008
  • Team:Caltech/Project/Vitamins
    ...cols/Folate assay|<font style="color:#BB4400">folate microbiological assay protocol</font>]] page. ...d not show any detectable PABA peaks. Samples were run using the same HPLC protocol as the standards, and both lysate and supernatant were tested for PABA peak
    23 KB (3,375 words) - 18:48, 20 October 2008
  • Imperial College/26 August 2008
    ...MPERIAL/2008/Prototype/Wetlab/Transformation_protocol_4 click here for the protocol], was not performed today. ...equire these midi preps to PCR out biobricks and for use in transformation protocol optimisation.
    2 KB (254 words) - 20:39, 28 October 2008
  • Team:Chiba/jk/β/weak 4
    >[[Team:Chiba/protocol/j|Protocols]] [[Team:Chiba/protocol/phenotype/timedelay/j|time-delay test]]
    2 KB (347 words) - 07:46, 30 September 2008
  • Team:Chiba/protocol/phenotype/timedelay/j
    >[[Team:Chiba/protocol/j|プロトコルページへ]]<Br> === プロトコル Protocol ===
    4 KB (434 words) - 09:28, 15 December 2008
  • Team:Chiba/Calendar-Home/6 September 2008
    '''[[Team:Chiba/protocol/PCR|Colony-PCR]]''' :--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
    4 KB (581 words) - 22:19, 29 October 2008
  • Team:Chiba/Calendar-Home/13 September 2008
    *[[Team:Chiba/protocol/phenotype/timedelay/j|time-delay test]] :'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
    4 KB (634 words) - 23:52, 29 October 2008
  • Team:Chiba/Calendar-Home/16 September 2008
    [[Team:Chiba/protocol/phenotype/timedelay/j|time-delay test]] with (15/9) cultures. [[Team:Chiba/protocol/gelcheck|Gel check]]
    3 KB (429 words) - 00:00, 30 October 2008
  • Team:Chiba/protocol/phenotype/timedelay
    >[[Team:Chiba/protocol|return to protocols page]]<Br> === Protocol ===
    3 KB (504 words) - 08:56, 30 October 2008
  • Team:Imperial College/Transformation 2
    {{Imperial/Box1|Transformation Protocol 2| ======Protocol======
    3 KB (562 words) - 17:39, 28 October 2008
  • Team:Imperial College/Transformation
    {{Imperial/Box1|Transformation Protocol 1| ======Protocol======
    4 KB (624 words) - 11:56, 28 October 2008
  • Team:Imperial College/Growth
    ...low proper characterisation of Promoters and RBSs for ''B.subitlis''. This protocol must give results that are as accurate as possible over a considerable rang ======Protocol======
    2 KB (402 words) - 17:41, 28 October 2008
  • Team:Imperial College/Fluorescence
    *The aim of this protocol is to produce a calibration curve of fluorescence vs molecules of GFPmut3b. ======Protocol======
    3 KB (489 words) - 17:42, 28 October 2008
  • Team:Harvard/Dailybook/Week4/Chemical and Light
    We followed the standard digest protocol under the "General Protocols" section. We used the standard digestion protocol.
    38 KB (5,639 words) - 19:03, 26 October 2008
  • Team:Michigan/Notebook/LigationProtocol
    == <font color=royalblue size=5> Ligation Standard Protocol </font> == =Protocol=
    2 KB (248 words) - 03:06, 30 October 2008
  • Team:Michigan/Notebook/PlasmidPrepProtocol
    == <font color=royalblue size=5> Plasmid Prep Standard Protocol </font> == =Protocol=
    747 B (105 words) - 03:18, 30 October 2008
  • Team:Cambridge/Bacillus subtilis transformation
    ...nfirm our transformations and to be able to standardize our protocol. This protocol is modified from the one provided by the Bacillus Genetic Stock Center (htt ''Protocol of the Amylase Test:''
    7 KB (1,049 words) - 20:39, 29 October 2008
  • Transformation and Plating
    ==Transformation Protocol== '''Heat transformation Protocol'''
    1 KB (233 words) - 04:33, 30 October 2008
  • Re-transform with selected plasmid
    ==Transformation Protocol== '''Heat Transformation Protocol'''
    1 KB (233 words) - 04:33, 30 October 2008
  • Sequence
    ==Sequencing Protocol== '''Protocol:'''
    840 B (111 words) - 01:00, 30 October 2008
  • PCR SOEing
    ==PCR SOEing Protocol== ...along with PCR Supermix and placed into a Thermocycler under the following protocol:
    1 KB (217 words) - 00:58, 30 October 2008
  • Team:Imperial College/Biomaterial Assay
    ======Protocol====== *'''Will finish this tomorrow when I finalise the protocol!!!!!!'''
    3 KB (471 words) - 00:28, 29 October 2008
  • Team:Freiburg Transfection and Synthetic Receptor
    ...f our parts was analyzed in three steps. First, we tested the transfection protocol and checked the utility of the transfection vector CMV construct for expres Transfection was performed with a lac Z gene using the transfection protocol described above. After 48h one part of the cells was harvested by washing t
    7 KB (1,055 words) - 23:04, 29 October 2008
  • TUDelft/16 October 2008
    ...oup). The machine used was a Thermo Savant FastPrep FP120 Homogenizer. The protocol used was:
    988 B (151 words) - 15:33, 27 October 2008
  • October
    The protocol of july 24th was used.<br> Preparation of the ssDNA was done following the QIAPrep Spin M13 Kits protocol. <br>
    45 KB (5,909 words) - 02:40, 30 October 2008
  • SOC
    ==Protocol==
    546 B (73 words) - 02:24, 30 May 2008
  • Team:UNIPV-Pavia/Protocols/PlasmidExtraction
    ...qiagen.com/literature/render.aspx?id=370&tp=4 QIAGEN QIAprep Spin Miniprep protocol]
    2 KB (325 words) - 12:52, 1 July 2008
  • Team:Hawaii/Tri-parental conjugation between E. coli and PCC6803
    == Protocol from Callahan Lab ==
    4 KB (637 words) - 06:56, 27 June 2008
  • Team:Montreal/DNA Agarose Gel
    ==Protocol==
    3 KB (394 words) - 19:57, 17 June 2008
  • Team:Montreal/Cell transformation
    ==Protocol==
    2 KB (247 words) - 19:41, 17 June 2008
  • Team:Montreal/Seeding
    ==Protocol==
    2 KB (239 words) - 19:40, 17 June 2008
  • Team:Hawaii/Protocols/Transformation PCC6803
    == Protocol ==
    3 KB (500 words) - 04:32, 24 June 2008
  • Team:MIT/Modeling
    ...familiarity, we had a hard time growing up the bacteria. We have written a protocol for growing strains of ''Lactobacillus'' [http://openwetware.org/wiki/Lacto ... the ''Lactobacillus''. From our research we found a electrotransformation protocol by Serror et al (App and Env Microbiology, 2002). Further research convince
    6 KB (897 words) - 04:21, 30 October 2008
  • Team:Hawaii/PCC6803 cell density
    == Protocol==
    1 KB (182 words) - 21:53, 27 June 2008
  • Team:University of Lethbridge/Notebook/Project1July
    ...wet ware [http://openwetware.org/wiki/Preparing_chemically_competent_cells protocol]. 100 uL aliquots of cells were frozen in liquid nitrogen and stored in th ...possibility one we will try more DNA. If that does not work we will try a protocol using electroporation.
    14 KB (2,039 words) - 02:32, 30 October 2008
  • Team:Paris/August 4
    ===Protocol of screening PCR===
    2 KB (223 words) - 15:22, 6 August 2008
  • Team:Hawaii/Protocols/oligo hybridization
    ===Protocol===
    637 B (87 words) - 21:59, 9 July 2008
  • Team:Hawaii/Protocols/gel extraction
    ===Protocol===
    1 KB (185 words) - 22:10, 9 July 2008
  • Team:Paris/July 31
    === Protocol ===
    5 KB (791 words) - 17:04, 13 August 2008
  • Team:ESBS-Strasbourg/Transformation Heat shock
    Protocol is a mixture of the IGEM standard protocol and the protocol used in the local lab<br>
    337 B (56 words) - 08:29, 5 August 2008
  • Team:ESBS-Strasbourg/11 July 2008
    ** competent Top10 cells from Invitrogen, protocol from Marias Lab: H2O (negative control), pxj (positive control), 5 BioBrick ** competent db3.1 cells, protocol from Barbaras Lab, DNA was already in KCM and cells were just added and pla
    1 KB (162 words) - 16:53, 16 July 2008
  • Team:ESBS-Strasbourg/Protocols
    ...-Strasbourg/Competent Bacteria | Preparation of competent cells (Barbara's Protocol)]] <br> ...sbourg/Transformation Barbara|Transformation of competent cells (Barbara's Protocol)]]<br>
    2 KB (192 words) - 00:13, 29 October 2008
  • Team:ESBS-Strasbourg/Miniprep: plasmid amplification
    ==b) Protocol ==
    2 KB (262 words) - 09:23, 18 July 2008
  • Team:ESBS-Strasbourg/Restriction digestion
    =b) Protocol = '''double digestion protocol used for plasmid verification 17/07/08 (KK):'''
    2 KB (333 words) - 13:28, 18 July 2008
  • Team:ESBS-Strasbourg/Ligation
    2)Protocol: <br> [http://openwetware.org/wiki/DNA_Ligation see iGEM protocol]
    586 B (91 words) - 08:02, 5 August 2008
  • Team:Hawaii/Biobrick conversions
    ==Protocol==
    9 KB (1,260 words) - 20:53, 15 August 2008
  • Team:TUDelft/25th of September protocol
    =25th of September Luciferase assay protocol= <b>This protocol has been discarded due to interfering of Promega lysis buffer with the prot
    2 KB (257 words) - 13:51, 20 October 2008
  • 15th of October protocol
    =15th of October luciferase assay protocol= <b>This protocol has been discarded due to the inconsistency of sonication amplitude</b>
    2 KB (260 words) - 13:47, 20 October 2008
  • Team:Paris/July 17
    ===Protocol===
    7 KB (1,035 words) - 14:35, 31 July 2008
  • Team:Hawaii/Protocols/ExoSAP
    ==Protocol==
    217 B (37 words) - 23:54, 18 July 2008
  • Team:IIT Madras/Project
    ===Standardized protocol for stress===
    7 KB (1,125 words) - 14:42, 27 October 2008
  • Team:Hawaii/Birds Eye View
    == [[Team:Hawaii/Protocols|Protocols]] ([[Template:Team:Hawaii/Protocol|t]]) ==
    5 KB (626 words) - 03:27, 30 October 2008
  • Team:University of Ottawa/31 July 2008
    :<li> Following protocol outlined online, tested a microwave protocol to hasten digestion period. ...romising; however, more experimentation needed to confirm validity of this protocol
    6 KB (801 words) - 15:05, 12 August 2008
  • Team:Paris/August 5
    === '''PCR Protocol''' ===
    4 KB (533 words) - 15:17, 6 August 2008
  • Team:Paris/July 30
    ===Protocol of screening PCR===
    19 KB (2,548 words) - 17:03, 13 August 2008
  • EPF-Lausanne/7 August 2008
    Ligation protocol : Following the protocol from OpenWetWare, but with our T4 ligase the ligation time is 4 hrs at 15 d
    1 KB (190 words) - 12:11, 7 August 2008
  • My Notes II
    ...t night about what to do with our non-cooperative sca2 and sca3. Got a new protocol for dealing with cotransformed sca2. Jin amplified fimH while he was still *8:45a.m. - miniprepped two of our co-transformed sca2 samples for a new protocol (to rid co-transformation)
    14 KB (2,169 words) - 07:56, 6 September 2008
  • Team:Paris/August 9
    ==='''Protocol of the ligation'''===
    3 KB (363 words) - 17:11, 13 August 2008
  • Imperial College/19 August 2008
    ...ion_Protocol_2| Click link here for protocol]. The basic principle of this protocol is that competent cells are prepared by growing a culture to a high O.D.<su
    1 KB (192 words) - 20:37, 28 October 2008
  • Imperial College/14 August 2008
    ...b/Transformation_protocol_4#Transformation_Protocol_2| Click link here for protocol] *The basic principle of this protocol is that competent cells are prepared by growing a culture to a high O.D.<su
    2 KB (339 words) - 20:36, 28 October 2008
  • Team:Paris/August 14
    ==== Protocol : ==== ...he [[Team:Paris/Notebook/Protocols#Purification_.28Kit_Promega.29|standard protocol]].
    3 KB (343 words) - 14:58, 16 August 2008
  • Team:Chiba/protocol/ligation/gelex
    >[[Team:Chiba/protocol|Protcol]] READ:[[Team:Chiba/protocol/gelcheck|Agarose gel electrophoresis]]
    410 B (64 words) - 03:10, 11 September 2008
  • Team:Caltech/Protocols/Folate assay
    * Previously, we had been using this protocol with the other folate-dependent strain ''Entercoccus hirae'' ATCC 8043, and ...sorbances of the samples at 546 nm on a plate reader (200uL per well). The protocol recommends refrigerating the samples at 4C for 15-30 minutes prior to readi
    6 KB (840 words) - 00:48, 30 October 2008
  • Team:Caltech/Protocols/Coculture Inhibition Assay
    ===Protocol===
    2 KB (233 words) - 01:06, 30 October 2008
  • Team:Paris/August 23
    [[Team:Paris/Notebook/Protocols#Transformation |Protocol]] [[Team:Paris/Notebook/Protocols#Digestion |Protocol]]
    4 KB (610 words) - 14:47, 6 September 2008
  • Team:University of Lethbridge/Notebook/Project2August
    -went over the SELEX protocol with HJ to determine the primers we will need to do this, and how exactly w Same as previous protocol, However, Annealing Temperature is set to 65.0 C
    6 KB (875 words) - 02:34, 30 October 2008
  • Team:Valencia/Project/Lab work/2 experiments
    The objective of this two-step protocol is to be able to start the experiment with a higher O.D. in a medium with l ...to do so, we designed the two inoculum protocol described above. With this protocol, we intend to reach an high O.D. without having too much glucose. We used S
    13 KB (1,943 words) - 00:44, 30 October 2008
  • Imperial College/17 September 2008
    ... link [http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Protocols/Growth#Protocol|here]. The following O.D.<sub>600</sub> and dilutions were used:
    1 KB (178 words) - 20:47, 28 October 2008
  • August
    ...tubes and after 20 minutes I started the transformation with the following protocol<br> There were no colonies on the plates so I changed the protocol and tried it again.<br>
    8 KB (1,289 words) - 02:40, 30 October 2008
  • September
    see at the protocol from 07-24-2008 see at the protocol from 07-24-2008
    8 KB (1,307 words) - 02:41, 30 October 2008
  • Team:Bologna/Biosafety
    ...he need for specific precautions to be adopted during the execution of the protocol. | '''Protocol'''
    29 KB (4,412 words) - 02:19, 30 October 2008
  • 25th of September protocol
    =25th of September Luciferase assay protocol= <b>This protocol has been discarded due to interfering of Promega lysis buffer with the prot
    2 KB (257 words) - 12:18, 20 October 2008
  • Team:Heidelberg/Parts/Characterization
    '''Protocol''' In this experiment we again used the same protocol as described above in test 1 but used different donor / recipient rations,
    87 KB (12,520 words) - 17:05, 29 October 2008
  • Team:Tokyo Tech/Result
    == Protocol ==
    1 KB (193 words) - 13:49, 26 October 2008
  • Team:Chiba/Calendar-Home/15 September 2008
    :'''[[Team:Chiba/protocol/transformation|Transformation]]''' (15/9)-->[[Team:Chiba/protocol/phenotype/T9002/j|T9002 check(AHL sensitivity and time response test)]]
    812 B (101 words) - 00:00, 30 October 2008
  • Team:Chiba/Calendar-Home/26 September 2008
    <br>colony [[Team:Chiba/protocol/PCR|PCR]] <br>->[[Team:Chiba/protocol/gelcheck|Gel check]]
    1 KB (194 words) - 01:08, 30 October 2008
  • IGEM:Cambridge/2008/Turing Pattern Formation/Experiments/Bacillus subtilis transfomation
    ...recise tests confirm our transformations and to be able to standardize our protocol ''Protocol of the amylase test''
    6 KB (969 words) - 22:24, 28 October 2008
  • Team:Chiba/parts/BBa K084008
    ====[[Team:Chiba/protocol/phenotype/timedelay|more details]]====
    2 KB (210 words) - 11:52, 28 October 2008
  • Team:Imperial College/XL1 Blue
    ======Protocol======
    3 KB (419 words) - 17:40, 28 October 2008
  • Team:Harvard/Dailybook/Week11/Chemical and Light
    ** Perhaps ligation protocol is not optimal (use method below) ** Ligations with old protocol failed
    4 KB (647 words) - 19:21, 26 October 2008
  • Team:Heidelberg/Notebook/Killing II/7thweek
    .... Because of that we started a new PCR with the right reverse primers (see protocol at pSB1A3-Receiver cloning) *Transformation of pSB1A3-T9002-without-GFP ON-Ligations (Transformation protocol Chris)
    37 KB (5,261 words) - 21:35, 28 October 2008
  • USTC/Notebook/Top10 chem competent cell transformation
    ===Regular Transformation protocol for Top10/DH5α cells===
    1 KB (201 words) - 09:54, 28 October 2008
  • Team:BrownTwo/Protocols/compcells
    ...ends to be even harder. We have had a great experience with the following protocol from Inoue, getting near lawns of transformants from ligations: Original protocol from Inoue et al.
    5 KB (756 words) - 08:51, 28 October 2008
  • Team:Michigan/Notebook/TransformationProtocol
    == <font color=royalblue size=5> Transformation Standard Protocol </font> ==
    2 KB (239 words) - 03:06, 30 October 2008
  • Team:Freiburg Transfection
    Transfection was performed with a lac z gene using the transfection protocol described above. After 48h one part of the cells was harvested by washing t <h4>'''Testing the transfection protocol'''</h4>
    7 KB (903 words) - 14:30, 29 October 2008
  • Team:Cambridge/Signaling/Experiments
    Again, the last option is most suitable for our investigation. Here's the protocol taken from Balaban et al (http://www.jbc.org/cgi/content/abstract/276/4/265 ***Guangyong Ji at Catholic University has a protocol for purification
    8 KB (1,194 words) - 15:08, 28 October 2008
  • USTC/Notebook/Chem competent cell transformation
    ===Regular Transformation protocol for Top10/DH5α cells===
    1 KB (201 words) - 15:37, 28 October 2008
  • Team:Cambridge/Protocols
    For the protocol see This protocol is designed to purify single- or double-stranded DNA fragments from PCR
    16 KB (2,728 words) - 20:12, 29 October 2008
  • PCR Amplification
    ==PCR Protocol==
    829 B (125 words) - 00:56, 30 October 2008
  • Digestion
    ==Digestion Protocol==
    995 B (165 words) - 00:58, 30 October 2008
  • Restriction Map/ Restriction Digest
    ==Restriction Digest Protocol==
    1 KB (229 words) - 01:00, 30 October 2008
  • CIP Treatment
    ==CIP Treatment Protocol==
    835 B (131 words) - 00:58, 30 October 2008
  • Ligate DNA
    ==Ligation Protocol==
    2 KB (323 words) - 00:59, 30 October 2008
  • Infection
    ==Infection Protocol==
    305 B (48 words) - 01:01, 30 October 2008
  • Lentivirus Production
    ==Virus Production Protocol==
    741 B (121 words) - 01:01, 30 October 2008
  • Lentivirus Harvesting
    ==Virus Harvesting Protocol==
    971 B (141 words) - 01:01, 30 October 2008
  • Transfection (nonviral)
    ==Transfection Protocol==
    609 B (99 words) - 01:02, 30 October 2008
  • Team:Chiba/protocol/ligation/gelex/j
    >[[Team:Chiba/protocol/j|プロトコルページへ]]<Br> これは[[Team:Chiba/protocol/gelcheck/j|電気泳動]]後からの操作です。
    2 KB (65 words) - 05:16, 27 June 2009
  • Team:Hawaii/Milestone
    * Culturing Protocol Optimization: CO2 is bottle neck, need incubator retrofitted (KS) * 2008.06.01 Plasmid Transformation Plan/Protocol (GK/MR)
    2 KB (219 words) - 17:35, 15 August 2008
  • Purdue/23 June 2008
    ...lpha-T1 cells (Invitrogen) and used a combo of their protocol and the iGEM protocol
    2 KB (391 words) - 14:27, 27 June 2008
  • Purdue/8 July 2008
    Yesterday, the two parts were prepped following the iGEM protocol (including the extra step). We used 2 spots of paper, and used 16uL of TE ...s. For the rest of the transformation process, we followed the Invitrogen protocol that is supplied with the cells:
    2 KB (286 words) - 19:06, 8 July 2008
  • Team:Hawaii/Test Competent E. Coli
    ...onential growth of E. coli seed stocks at 23C (Callahan cells, recommended protocol) result in higher competency than growth of E. coli at 37C (our cells)? ... cells were made on 7/11/08 with this [[Team:Hawaii/Make Competent E. Coli|protocol]].
    4 KB (614 words) - 01:33, 16 July 2008
  • Team:Purdue/Project
    ...hat they cannot survive outside of a lab environment. In other words, our protocol was entirely under Biosafety Level I (i.e., little or no health hazard). ...IBC). In cooperation with them, we filled out a form detailing our entire protocol. We then had to indicate any health hazards that could be posed by our pro
    12 KB (1,859 words) - 23:49, 29 October 2008
  • Team:UNIPV-Pavia/Protocols/GelExtraction
    ...-science.com/pack-insert/1696505a.pdf Roche Agarose Gel DNA Extraction Kit protocol]
    2 KB (266 words) - 12:52, 1 July 2008
  • Team:Hawaii/Initial BioBrick Plasmid Extraction From Filter Paper
    ==Protocol== Protocol adapted from Registry of Standard Biological Parts, [http://partsregistry.o
    3 KB (443 words) - 07:18, 12 June 2008

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