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  • Team:Chiba/jk/β/weaks 1-2
    :'''[[Team:Chiba/protocol/transformation|Transformation]]''' :'''[[Team:Chiba/protocol/PCR|PCR]]'''
    43 KB (6,909 words) - 07:07, 20 September 2008
  • Team:Chiba/jk/β/week 2
    >[[Team:Chiba/protocol/j|Protocols]] :(22/8)--->'''[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]'''
    22 KB (3,468 words) - 12:11, 23 October 2008
  • Team:Chiba/notebook/input/august
    '''[[Team:Chiba/protocol/PCR|PCR]]''' -->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
    17 KB (2,289 words) - 05:02, 24 October 2008
  • Team:Chiba/jk/β/week 3
    >[[Team:Chiba/protocol/j|Protocols]] :'''[[Team:Chiba/protocol/transformation|Transformation]]'''
    26 KB (4,447 words) - 04:05, 24 October 2008
  • Team:Chiba/Calendar-Home/30 August 2008
    '''[[Team:Chiba/protocol/digestion|Digestion]]''' -->30μl'''[[Team:Chiba/protocol/ligation/gelex|Gel extraction]]'''
    11 KB (1,784 words) - 23:13, 29 October 2008
  • Team:Chiba/protocol/j
    ===[[Team:Chiba/protocol/transformation/j|Z コンピ]] === ===[[Team:Chiba/protocol/DNA_Purification/sigma/j|シグマプレップ]]===
    4 KB (188 words) - 05:02, 27 June 2009
  • Team:Cambridge/Bacillus/Lab Work
    '''Protocol''' *Add Disks in antibiotic solution for 5 mins [ Protocol to sterilize tweezers : Wipe with Kimwipes, Ethanol, Flame ]
    65 KB (9,746 words) - 03:56, 30 October 2008
  • Team:Chiba/protocol
    ===[[Team:Chiba/protocol/transformation|Zymo comp.]] === ===[[Team:Chiba/protocol/transformation|Electro poration]]===
    1 KB (181 words) - 12:46, 16 December 2008
  • Team:iHKU/protocol
    <td><a href="/Team:iHKU/protocol"><img src="/wiki/images/f/f2/Button_protocol.jpg" width="200" height="25" / <li> PROTOCOL-1 <a href="#p1">Pattern formation</a></li>
    63 KB (6,466 words) - 19:25, 29 October 2008
  • Team:Chiba/jk/β/week 1
    >[[Team:Chiba/protocol/j|Protocols]] :'''[[Team:Chiba/protocol/transformation|Transformation]]'''
    22 KB (3,633 words) - 12:34, 30 September 2008
  • Team:Chiba/jk/β/week 5
    >[[Team:Chiba/protocol/j|Protocols]] :(13/9)--->'''[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]'''
    6 KB (804 words) - 13:20, 1 October 2008
  • GenProtocols
    This protocol is designed for the purification of up to 20 ug high-copy plasmid DNA from ==Ligation protocol, using Roche Rapid Ligation Kit==
    17 KB (2,715 words) - 19:24, 26 October 2008
  • Team:BrownTwo/Notebook
    ...or about 4.5 hours since other obligations prevented us from finishing the protocol. Normally, we would have plated the transformations immediately after they <p>Gel Mixture Protocol for 1% Agarose gel:</p>
    226 KB (32,672 words) - 04:53, 29 October 2008
  • Team:Paris/August 8
    ==== '''Protocol''' ==== ==== Protocol : ====
    18 KB (2,438 words) - 13:22, 15 August 2008
  • Team:Chiba/jk/β/weaks 3-4
    :'''[[Team:Chiba/protocol/transformation|Transformation]]''' '''[[Team:Chiba/protocol/digestion|Digestion]]'''
    13 KB (2,259 words) - 07:03, 20 September 2008
  • Team:Harvard/GenProtocols
    ==Ligation protocol, using Roche Rapid Ligation Kit== ===Brief Protocol===
    13 KB (2,102 words) - 05:38, 29 October 2008
  • Team:Paris/August 15
    ==='''Protocol'''=== ...ly competent cells [[Team:Paris/Notebook/Protocols#Transformation|standard protocol]].
    9 KB (1,174 words) - 18:06, 4 September 2008
  • Team:Chiba/Calendar-Home/1 September 2008
    '''[[Team:Chiba/protocol/gelcheck|Gel Check]]''' '''[[Team:Chiba/protocol/transformation|Transformation]]'''(Double Transformation)
    8 KB (1,254 words) - 01:42, 30 October 2008
  • Team:Chiba/jk/γ/week 2
    [[Team:Chiba/protocol/transformation|Transformation]] [[Team:Chiba/protocol/digestion|Digestion]]
    9 KB (1,396 words) - 05:50, 24 October 2008
  • Team:Chiba/Calendar-Home/26 August 2008
    :(8/25)--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]''' --->'''[[Team:Chiba/protocol/ligation/gelex|Gel Extract]]'''
    7 KB (1,128 words) - 01:04, 30 October 2008
  • Team:Calgary Wetware/Protocols
    ...I wrote up a procedure for diluting primers. As an added bonus, contains a protocol for PCR purification! [[Team:Calgary_Wetware/Protocols/Diluting|Check it ou <li><a href="#transformation" title="Bacterial Transformation Protocol"> Bacterial Transformation </a></li>
    23 KB (3,681 words) - 23:18, 29 October 2008
  • Team:TUDelft/Protocols
    ...We did make a stock of chemically competent DB3.1 cells with the following protocol (found on OpenWetWare). We found that these cells were indeed very competen ''Protocol found on OpenWetWare''
    19 KB (3,279 words) - 23:04, 29 October 2008
  • Team:KULeuven/Data/Other Parts
    ...s can be tested (the construct that have to be made and the actual testing protocol). It will also give an overview of the results we had so far. We worked as === Construct & protocol ===
    14 KB (2,307 words) - 23:28, 29 October 2008
  • Team:Chiba/jk/β/week 4
    [[Team:Chiba/protocol/digestion|Digestion Test]] :--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
    5 KB (714 words) - 12:52, 30 September 2008
  • Team:Paris/August 6
    ==== Protocol ==== ====Protocol====
    8 KB (1,222 words) - 14:06, 13 August 2008
  • Team:Illinois/Bimolecular Fluorescence Biosensor Notebook
    ** PCR protocol as in paper for both N terminal * Gel Extraction Protocol
    14 KB (2,108 words) - 18:50, 29 October 2008
  • Team:Chiba/Calendar-Home/28 August 2008
    '''[[Team:Chiba/protocol/transformation|Transformation]]''' '''[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]'''
    5 KB (757 words) - 03:14, 30 October 2008
  • July
    -&gt; protocol 07-10-2008 <br> we repeated step 5 and 6 of the protocol.<br>
    22 KB (3,483 words) - 02:40, 30 October 2008
  • Team:Imperial College/Light Inducible
    The aim of this protocol is to characterise the light induced expression of GFPmut3b under the contr ======Protocol======
    6 KB (938 words) - 17:44, 28 October 2008
  • Team:Hawaii/Make Competent E. Coli
    ...at6709852.pdf | Bloom04]] patent describes the use of essentially the same protocol for the Invitrogen Mach 1 cells. '''This is the chemical transformation protocol used by [[User:Tk|Tom Knight]] and the [http://partsregistry.org Registry o
    7 KB (1,158 words) - 23:54, 30 May 2008
  • Team:University of Lethbridge/Notebook/Project1August
    ...ok & Russell, (2001) ''Molecular Cloning: A Laboratory Manual 3rd Edition. Protocol 25'' '''Vol. 1; 1.117'''): Protocol:
    13 KB (1,866 words) - 02:06, 30 October 2008
  • Team:Paris/August 27
    [[Team:Paris/Notebook/Protocols#Concentration_of_the_Miniprep | Protocol (it's same that for Miniprep)]] [[Team:Paris/Notebook/Protocols#Digestion|Protocol Digestion]]
    5 KB (688 words) - 19:16, 9 September 2008
  • Team:KULeuven/Data/Other Components
    ... can be tested (the constructs that have to be made and the actual testing protocol). It will also give an overview of the results we had so far. We worked as === Construct & protocol ===
    9 KB (1,463 words) - 20:13, 29 October 2008
  • Team:Hawaii/Initial PCC6803 Transformation
    == Protocol by Arizona State University == == Protocol by Shao, ''et al'' ==
    4 KB (628 words) - 06:54, 27 June 2008
  • Team:Hawaii/Initial E. Coli Transformation
    == Protocol by Invitrogen == == Protocol from SC Lab ==
    6 KB (893 words) - 02:31, 2 August 2008
  • Team:Paris/August 7
    *Concerning '''pflhB, pflgA and pflgB''', the protocol seems to be '''very operational''': we always have great results ! *Standart protocol except the last step. Instead of eluting with water, we used 30 µL of BE b
    10 KB (1,412 words) - 12:42, 13 August 2008
  • Team:Illinois/Antibody GPCR Fusion Notebook
    **Protocol from Wiley's ''Current Protocols in Molecular Biology'' ** Result: Failed to finish protocol
    13 KB (1,969 words) - 02:55, 30 October 2008
  • Team:Paris/August 22
    [[Team:Paris/Notebook/Protocols#Digestion|Protocol Digestion]] [[Team:Paris/Notebook/Protocols#Migration after extraction |Protocol]]
    7 KB (891 words) - 00:54, 21 September 2008
  • Team:Chiba/Calendar-Home/29 August 2008
    :--->'''[[Team:Chiba/protocol/digestion|Digestion Test]]''' :--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
    5 KB (803 words) - 20:28, 29 October 2008
  • Team:Paris/August 21
    ==Protocol== [[Team:Paris/Notebook/Protocols#Digestion|Protocol Digestion]]
    7 KB (836 words) - 14:29, 6 September 2008
  • Team:Paris/August 26
    ==Protocol== '''Protocol'''
    11 KB (1,582 words) - 17:59, 11 September 2008
  • Team:Chiba/jk/γ/week 3
    [[Team:Chiba/protocol/digestion|Digestion]] [[Team:Chiba/protocol/ligation/ligation|Ligation]]
    6 KB (946 words) - 06:30, 24 October 2008
  • Team:Chiba/jk/γ/week 4
    [[Team:Chiba/protocol/digestion|Digestion]] -->[[Team:Chiba/protocol/ligation/gelex|Gel Extraction]]
    6 KB (907 words) - 06:52, 24 October 2008
  • Team:TUDelft/Temperature results
    ...ure_results#Setting_up_luciferase_measurements|Obtaining a correct working protocol to measure luminescence]] ...onstruction of all devices the 3A assembly strategy was chosen. A complete protocol of this strategy can be found on [http://openwetware.org/wiki/Synthetic_Bio
    21 KB (3,339 words) - 10:56, 30 October 2008
  • Team:Chiba/Calendar-Home/19 September 2008
    '''[[Team:Chiba/protocol/gelcheck|Gel Check]]''' --->'''[[Team:Chiba/protocol/ligation/gelex|Gel Extract]]'''<br>
    3 KB (476 words) - 00:20, 30 October 2008
  • Team:Imperial College/Transformation Results
    ...m:Imperial_College/Transformation Click this link for full Transformation protocol 1 '''] '''Brief Description of Protocol'''<br>
    4 KB (559 words) - 01:59, 30 October 2008
  • Team:Michigan/Notebook/PCRProtocol
    == <font color=royalblue size=5> PCR Standard Protocol </font> == ... machines may also require different mixtures to run properly. This is the protocol specified for our machine, the PCR Express.
    2 KB (365 words) - 03:18, 30 October 2008
  • Team:Cambridge/Signalling/Lab Work
    * found protocol for Bacillus subtilis transformation ** Miniprep I746001 and I746101 pellets (plasmid mniprep kit) : same protocol but 60μL of Zyppy Glution Buffer in step 8
    36 KB (5,743 words) - 04:15, 30 October 2008
  • Extract DNA (Maxiprep or Midiprep)
    ==Midiprep/ Maxiprep Growth Protocol== ==Midiprep Protocol (QIAGEN kit)==
    6 KB (1,068 words) - 01:00, 30 October 2008
  • Team:Hawaii/Initial Synth. Oligo Assembly
    ==Protocol== ...e.org/wiki/Engineering_BioBrick_vectors_from_BioBrick_parts/Colony_PCR her protocol on OpenWetWare]. No visible difference was detected between the three annea
    19 KB (3,019 words) - 16:40, 25 July 2008
  • Team:Beijing Normal/Project
    == Special protocol == ...ng to complete failure. To solve this problem, we have developed a special protocol-- hot start method combined with additive(sole or mixed)-- which is most he
    18 KB (2,612 words) - 04:27, 30 October 2008
  • Team:Paris/August 20
    *[[Team:Paris/Notebook/Protocols#Glycerol stocks| Protocol stocks]] *[[Team:Paris/Notebook/Protocols#Minipreps (Kit_Qiagen)| Protocol Miniprep]]
    9 KB (1,183 words) - 14:03, 6 September 2008
  • Team:Chiba/Calendar-Home/20 August 2008
    '''[[Team:Chiba/protocol/PCR|PCR]]''' -->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
    8 KB (1,334 words) - 05:57, 30 October 2008
  • Team:Chiba/Calendar-Home/25 August 2008
    :(24/8)--->'''[[Team:Chiba/protocol/digestion|Digestion]]''' :--->[[Team:Chiba/protocol/gelcheck|Gel Check]]
    7 KB (1,106 words) - 06:15, 30 October 2008
  • Team:Chiba/Calendar-Home/27 August 2008
    [[Team:Chiba/protocol/digestion|Digestion Test]] :--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
    4 KB (608 words) - 20:14, 29 October 2008
  • Team:Harvard/Dailybook/Week3/Chemical and Light
    ... 2008 registries. We made the following changes to the iGEM transformation protocol: After the gel extraction (standard kit protocol), the negative control (gel with no DNA) read 5.4 ng/μL and the positive c
    30 KB (4,485 words) - 19:02, 26 October 2008
  • Team:Bologna/UV radiation/
    ==Protocol used for UV induction== ...id with our construct has been transformed in DH5alfa bacteria by standard protocol and one colony from the plate has been picked and cultured overnight in 5m
    12 KB (2,040 words) - 09:00, 29 October 2008
  • DNA restriction digest
    List reagents, supplies and equipment necessary to perform the protocol here. For those materials which have their own OWW pages, link to that pag <!-- If this protocol has papers or books associated with it, list those references here. -->
    4 KB (515 words) - 01:22, 17 July 2008
  • Team:ETH Zurich/Wet Lab/Protocol
    ...<td><table><tr><td width=5px></td><td>[[Team:ETH_Zurich/Wet%20Lab/Protocol|Protocol]]</td></tr><tr><td width=5px></td><td><table></table></td></tr></table></td ...><span lang="en-US" id="d8on110">gel extraction was performed according to protocol:</span></p>
    68 KB (10,408 words) - 13:17, 18 August 2008
  • Team:EPF-Lausanne/Notebook
    We followed the protocol coming from [http://openwetware.org/wiki/TOP10_chemically_competent_cells O ...alculations, follow [http://openwetware.org/wiki/DNA_ligation DNA Ligation Protocol on OWW]
    16 KB (2,322 words) - 23:27, 29 October 2008
  • Team:Paris/August 1
    * Use of Quiagen's protocol on all the clones cultivated on the 31th. === Protocol ===
    10 KB (1,668 words) - 16:35, 23 August 2008
  • Team:Chiba/Calendar-Home/31 August 2008
    :'''[[Team:Chiba/protocol/transformation|Transformation]]''' :'''[[Team:Chiba/protocol/digestion|Digestion]]'''
    5 KB (713 words) - 20:41, 29 October 2008
  • Imperial College/17 August 2008
    *Prepare media and reagents for the protocol transformation 1 protocol *Prepare competent cells using the transformation 2 protocol, this time growing cells to an o.D.<sub>600</sub>
    3 KB (348 words) - 20:37, 28 October 2008
  • Team:Heidelberg/Notebook/team meetings
    ... || [[Image:text-icon.png|20px]] [[Media:protocol_20081021.txt | Protocol]]|| [[Image:icon-ppt.png|20px]] Report Phages | || [[Image:pdf-logo.jpg|20px]] [[Media:protocol_20081014.pdf | Protocol]]
    24 KB (3,294 words) - 17:13, 29 October 2008
  • Team:Chiba/Calendar-Home/2 September 2008
    :(31/8)--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]''' :(1/9)--->'''[[Team:Chiba/protocol/PCR| Colony PCR]]'''
    6 KB (905 words) - 03:10, 30 October 2008
  • Team:Chiba/Calendar-Home/14 September 2008
    (13/9)--->'''[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]''' (13/9)--->[[https://2008.igem.org/Team:Chiba/protocol/ligation/dephosphorylation|SAP]]
    3 KB (440 words) - 23:59, 29 October 2008
  • Team:Imperial College/Constitutive
    *To characterise the constitutive promoter and RBS combinations. This protocol is concerned with characterisation of the steady-state expression of GFPmut *The protocol below describes what is needed for one combination of promoter and RBS, we
    3 KB (559 words) - 17:43, 28 October 2008
  • Team:Imperial College/Inducible
    *The aim of this protocol is to help us define the dynamic range of the transfer function where the l *This protocol is to be used to characterise the IPTG inducible Hyper-spank promoter RBS c
    3 KB (495 words) - 17:39, 28 October 2008
  • Team:Harvard/Dailybook/Week0
    *Miniprep vectors ([[Team:Harvard/GenProtocols#QIAprep_Spin_Miniprep_Kit|protocol]]) ...d/GenProtocols#Chemically_competent_cells|protocol]]) - actually used Zymo protocol which didn't work for Shewie and I don't think for E. coli
    17 KB (1,690 words) - 17:34, 28 October 2008
  • Team:Hawaii/Protocols/Competent Cells
    ...at6709852.pdf | Bloom04]] patent describes the use of essentially the same protocol for the Invitrogen Mach 1 cells. '''This is the chemical transformation protocol used by [[User:Tk|Tom Knight]] and the [http://partsregistry.org Registry o
    5 KB (802 words) - 04:22, 24 June 2008
  • Team:MIT
    ...:MIT/Transforming_Lactococcus lactis|''Lactococcus lactis'' Transformation Protocol]] ...L._acidophilus_transformation ''Lactobacillus acidophilus'' Transformation Protocol]
    7 KB (996 words) - 09:46, 30 October 2008
  • Team:Hawaii/Large-Scale Preparation of Plasmid from E. coli
    ...can be obtained by using CsCl/ethidium bromide equilibrium centrifugation (protocol found in Maniatis). #*The protocol recommends using 6,000 x g.
    10 KB (1,527 words) - 08:31, 10 July 2008
  • Team:University of Lethbridge/Notebook/GeneralLabJuly
    Protocol changes: ...n from the filter paper. Next week we will attempt various changes to the protocol to extract more DNA.
    6 KB (838 words) - 02:29, 30 October 2008
  • Team:Paris/July 28
    === Protocol === === PCR Protocol ===
    20 KB (2,506 words) - 16:59, 13 August 2008
  • Team:Paris/July 29
    === Protocol === === Protocol ===
    9 KB (1,357 words) - 17:01, 13 August 2008
  • Team:Paris/August 12
    ====Protocol==== ...was two fragments, the standard [[Team:Paris/Notebook/Protocols#Extraction|protocol]]
    11 KB (1,508 words) - 11:00, 15 August 2008
  • Team:Paris/August 16
    ...he [[Team:Paris/Notebook/Protocols#Purification_.28Kit_Promega.29|standard protocol.]] ...ed the DNA following the [[Team:Paris/Notebook/Protocols#Ligation|standard protocol]].
    5 KB (652 words) - 18:08, 4 September 2008
  • Team:Paris/August 18
    *[[Team:Paris/Notebook/Protocols#Glycerol stocks| Protocol stocks]] *[[Team:Paris/Notebook/Protocols#Minipreps (Kit_Qiagen)| Protocol minprep]]
    5 KB (622 words) - 19:50, 4 September 2008
  • Imperial College/2 September 2008
    ...://openwetware.org/index.php?title=IGEM:IMPERIAL/2008/Prototype/Wetlab/PCR protocol], using the optimal conditions determined yesterday and results run on a ge *The results of this protocol are shown on figure 1 a 1% agarose gel. The lanes concerned are 1-4 and rep
    4 KB (654 words) - 20:43, 28 October 2008
  • Team:University of Lethbridge/Notebook/Project1October
    ...ation of BL21 and RP1616 with pUC19 (positive control) and pTopp using the protocol Jaden and Selina used on August 25, 2008. Protocol (from Kothe Lab):
    10 KB (1,545 words) - 02:01, 30 October 2008
  • Team:Chiba/Calendar-Home/22 August 2008
    '''[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]''': insert check:'''[[Team:Chiba/protocol/digestion|Digestion]]'''
    4 KB (576 words) - 03:02, 30 October 2008
  • Team:Chiba/jk/γ/week 5
    [[Team:Chiba/protocol/gelcheck|Gel check]] [[Team:Chiba/protocol/gelcheck|Gel check]]
    7 KB (1,028 words) - 07:14, 24 October 2008
  • Team:Chiba/Calendar-Home/3 September 2008
    :(2/9)--->'''[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]''' :--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
    7 KB (1,339 words) - 21:03, 29 October 2008
  • Team:Chiba/Calendar-Home/8 September 2008
    '''[[Team:Chiba/protocol/ligation/gelex|Gel Extract]]''' :--->'''[[Team:Chiba/protocol/DNA Purification/zymocleam|Zymo Clean]]'''
    4 KB (631 words) - 23:43, 29 October 2008
  • Team:Chiba/Calendar-Home/9 September 2008
    (6/9)--->[[Team:Chiba/protocol/PCR|PCR]] of [http://partsregistry.org/Part:BBa_I9030 BBa_I9030] --->'''[[Team:Chiba/protocol/ligation/gelex|Gel Extract]]'''
    3 KB (546 words) - 01:26, 30 October 2008
  • Team:Chiba/Calendar-Home/10 September 2008
    '''[[Team:Chiba/protocol/PCR|Colony-PCR]]''' -->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
    3 KB (476 words) - 23:10, 29 October 2008
  • Team:Chiba/Calendar-Home/18 September 2008
    *[[Team:Chiba/protocol/phenotype/timedelay/j|time-delay test]] [[Team:Chiba/protocol/ligation/dephosphorylation|SAP]] 1&mu;L
    3 KB (380 words) - 00:01, 30 October 2008
  • Team:Chiba/Calendar-Home/25 September 2008
    <br>colony [[Team:Chiba/protocol/PCR|PCR]] <br>[[Team:Chiba/protocol/digestion|Digestion test]]
    4 KB (667 words) - 23:29, 29 October 2008
  • Team:Heidelberg/Notebook/Killing I/Notebook/week9
    PCR protocol PCR protocol
    33 KB (4,755 words) - 11:45, 29 October 2008
  • Team:NYMU-Taipei/Project/Phosphate/Experiments/GEL Electrophoresis
    ! Protocol ! Protocol
    27 KB (3,602 words) - 20:00, 29 October 2008
  • Team:Michigan/Notebook/GelProtocol
    == <font color=royalblue size=5> Analytical Gel Standard Protocol </font> == == <font color=royalblue size=5> Gel Extraction Standard Protocol </font> ==
    1 KB (189 words) - 03:20, 30 October 2008
  • Run Gel/ Gel extraction
    ==Run Gel Protocol== ==Gel Extraction Protocol using QIAquick Gel Extraction Kit:==
    3 KB (453 words) - 00:56, 30 October 2008
  • Team:Illinois/Antibody GPCR Fusion
    Protocol for Homologous Recombination [http://www.mrw.interscience.wiley.com/emrw/97 - We can use Basic Protocol 2 or 3
    21 KB (3,227 words) - 02:19, 24 October 2008
  • Imperial College/3 September 2008
    ...n 60°C for 20 cycles. Aad9 PCR was again tested according to the standard protocol and agin at 52°C for the first 10 cycles then 58°C or 56°C for 20 cycles ...k firstly if the primers are working and secondly if the single colony PCR protocol is working we carried out a series of testing. First to test the verificat
    7 KB (1,168 words) - 20:44, 28 October 2008
  • Team:Hawaii/Protocols/Plasmid prep
    == Miniprep Protocol== #*The protocol recommends using 6,000 x g.
    5 KB (806 words) - 04:22, 1 September 2008
  • EPF-Lausanne/16 July 2008
    Try new transformation with some iGEM part which we don't need and adjust protocol. We need to redo the transformation to troubleshoot. Perhaps the protocol which we were using was not the right one, so we modify it according to the
    2 KB (422 words) - 12:14, 7 August 2008
  • Team:ETH Zurich/Wet Lab/Protocoll
    ...><span lang="en-US" id="d8on110">gel extraction was performed according to protocol:</span></p> ...bottom: 0in;"><span lang="en-US" id="yxzy4">repitition of PCR with changed protocol</span></div>
    68 KB (10,408 words) - 14:52, 3 August 2008
  • Team:Paris/July 25
    * Use of Promega's protocol for the extraction of: gel n°1 --> 4 : (D100 --> D110 cl-1 ES)) * Use of Qiagen's protocol for the extraction of: gel n°5 --> 8 : (D110 cl-2 ES --> D120))
    2 KB (334 words) - 15:32, 31 July 2008
  • Team:Bologna/Notebook
    ...he new [http://partsregistry.org/Measurement Biobrick Standard Measurement Protocol] * Protocol design for isolation of single operators from the library.
    15 KB (2,315 words) - 01:13, 30 October 2008
  • Team:Imperial College/Protocols
    *[[Team:Imperial College/Transformation |Protocol 1]] *[[Team:Imperial College/Transformation_2 |Protocol 2]]
    2 KB (199 words) - 21:14, 29 October 2008
  • EPF-Lausanne/30 July 2008
    ==2 Step PCR Protocol== ... around the edges. They were left to bake overnight at 80°C following the protocol.
    4 KB (592 words) - 12:12, 7 August 2008
  • Team:Chiba/Calendar-Home/18 August 2008
    '''[[Team:Chiba/protocol/transformation|Transformation]]''' :'''[[Team:Chiba/protocol/PCR|PCR]]'''
    3 KB (451 words) - 05:13, 30 October 2008
  • Imperial College/21 August 2008
    ...b/Transformation_protocol_4#Transformation_Protocol_2| Click link here for protocol]. However, colonies were seen on the plates were no DNA was used for transf ..._protocol_2| click here for a link to the protocol]. The principle of this protocol is that a culture is grown to a high O.D.<sub>600</sub>, this induces the s
    3 KB (439 words) - 20:38, 28 October 2008
  • Team:Chiba/Calendar-Home/21 August 2008
    :(20/8)--->'''[[Team:Chiba/protocol/PCR|PCR]]''' :--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
    4 KB (652 words) - 05:58, 30 October 2008
  • Wiki/Team:Warsaw/protocols
    ...ref="http://www.aabiot.com/pdf/protocols/dna_pur/plasmid/plasmid_mini.pdf">protocol</a> of producer.</p> ...a href="http://www.aabiot.com/pdf/protocols/dna_pur/fragments/gel_out.pdf">protocol</a> of producer.</p>
    17 KB (2,920 words) - 00:16, 30 October 2008
  • Team:Chiba/jk/β/weak 5
    :(13/9)--->'''[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]''' :'''[[Team:Chiba/protocol/transformation|Transformation]]'''
    2 KB (241 words) - 18:41, 24 September 2008
  • TUDelft/19 September 2008
    ===Protocol=== The protocol used in the case of most samples, when lysed by sonification was:<br>
    4 KB (550 words) - 11:23, 22 September 2008
  • Team:Chiba/jk/γ/week 1
    [[Team:Chiba/protocol/PCR|PCR]] [[Team:Chiba/protocol/transformation|Transformation]]
    3 KB (496 words) - 05:25, 24 October 2008
  • Team:Newcastle University/Bacillus subtilis
    ...bility in the ''B. subtilis'' culture prior to transformation. We used the protocol below to achieve this. ...s transient, and therefore observing correct timings in the transformation protocol is very important in increasing the population of competent cells.
    14 KB (1,803 words) - 21:35, 29 October 2008
  • Team:Chiba/Calendar-Home/23 August 2008
    :(21/8)--->'''[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]''' :--->'''[[Team:Chiba/protocol/digestion|Digestion test]]'''
    6 KB (927 words) - 06:02, 30 October 2008
  • Team:Chiba/Calendar-Home/24 August 2008
    :(22/8)--->'''[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]''' :--->'''[[Team:Chiba/protocol/digestion|Digestion test]]'''
    5 KB (838 words) - 06:07, 30 October 2008
  • Team:Chiba/Calendar-Home/4 September 2008
    :--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]''' :--->'''[[Team:Chiba/protocol/digestion|Digestion test]]'''
    4 KB (538 words) - 21:04, 29 October 2008
  • Team:Chiba/Calendar-Home/7 September 2008
    [[Team:Chiba/protocol/digestion|Digestion Test]] :--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
    4 KB (654 words) - 22:24, 29 October 2008
  • Team:Chiba/Calendar-Home/17 September 2008
    *[[Team:Chiba/protocol/phenotype/timedelay/j|time-delay test]] *[[Team:Chiba/protocol/phenotype/timedelay/j|time-delay test]]
    2 KB (342 words) - 22:35, 29 October 2008
  • Team:Chiba/Calendar-Home/20 September 2008
    --->(19/9)'''[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]] --->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
    2 KB (339 words) - 00:25, 30 October 2008
  • Team:Heidelberg/Notebook/Killing I/Notebook/week5
    ** protocol ** protocol see parameter measurement protocol
    31 KB (4,359 words) - 11:49, 29 October 2008
  • Team:Imperial College/PCR
    This protocol is desgined for use with the stratagene ''PfuUltra'' II Fusion DNA polymera A modified protocol for using Taq polymerase can be used if less fidelity is required, to obtai
    6 KB (1,016 words) - 01:27, 29 October 2008
  • Team:Harvard/Dailybook/Week5/Chemical and Light
    ... green dot on top) aliquots from 1L of culture. Done as per Jason's lab's protocol. ...o new dh5α competent cells w/ volumes of 1, 3, and 5uL, using Jason's new protocol. As a control transformed 1uL of pUC19.
    41 KB (5,434 words) - 19:03, 26 October 2008
  • Team:Harvard/Dailybook/Week6/Chemical and Light
    ...emove the PstI site from cph1 (P87A and B) according to the QuikChange kit protocol. We ran a PCR positive control and a transformation positive control. We tr We used the TOP10 transformation protocol for both cell types. We plated 125 μL of each sample on two plates.
    27 KB (4,294 words) - 19:18, 26 October 2008
  • Team:Heidelberg/Notebook/Killing II/2ndweek
    *Transformation of BBa_F2621 and BBa_F2622 with Registry protocol ...f BBa_T9002, BBa_F2621, BBa_F2622 and pColE9-J into MG1655 (Transformation protocol Chris)
    28 KB (3,873 words) - 21:21, 28 October 2008
  • EPF-Lausanne/29 September 2008
    #*PCR protocol: #*PCR protocol:
    2 KB (387 words) - 19:32, 28 October 2008
  • Team:Michigan/Notebook/DigestProtocol
    == <font color=royalblue size=5> Digest Standard Protocol </font> == =Digest Protocol=
    2 KB (337 words) - 03:03, 30 October 2008
  • Team:Bologna/Wetlab
    So, we decided to set a new protocol up for the isolation and cloning of single operator sites into standard Bio According to the protocol to test operator parts ([https://2008.igem.org/Team:Bologna/Modeling#Proced
    20 KB (3,124 words) - 12:57, 4 December 2008
  • Extract DNA (Miniprep)
    ==Miniprep Protocol (QIAGEN Miniprep kit)== '''Miniprep Growth Protocol'''
    4 KB (626 words) - 00:59, 30 October 2008
  • Team:Cambridge/Voltage/Lab Work
    ...th on the plates spread yesterday. There has been a few adjustments to the protocol. The results of yesterday's modified protocol still yielded no growth on the plates. Using yesterday's PCR product our ai
    21 KB (3,118 words) - 23:31, 29 October 2008
  • Team:University of Sheffield/Dimtry
    ===Original Protocol=== ...al protocol of CAI production, which is a slightly modified version of the protocol described in the paper,[1] was as follows:
    4 KB (530 words) - 13:15, 29 October 2008
  • Team:MIT/Experiments
    ...:MIT/Transforming_Lactococcus lactis|''Lactococcus lactis'' Transformation Protocol]] ...L._acidophilus_transformation ''Lactobacillus acidophilus'' Transformation Protocol]
    1 KB (153 words) - 01:22, 30 October 2008
  • Team:Tokyo Tech/Protocol
    !align="center" width=20% style="text-align:center"|[[Team:Tokyo_Tech/Protocol|Protcol]] == <font size=5>'''Protocol''' </font>==
    4 KB (554 words) - 04:42, 30 October 2008
  • Purdue/11 July 2008
    ... have 100% glycerol (sterile), so we're just going to follow the same iGEM protocol but with 100% glycerol *Dr. Clase also suggested a somewhat different protocol for making stocks, so we're going to do both side-by-side and see which is
    1 KB (168 words) - 17:31, 11 July 2008
  • Team:UNIPV-Pavia/Notebook/Week3
    ...lasmid digestion again for our 4 plasmids to open, following the digestion protocol (read Protocols section for details). ... well for BBa_B0030, whose fragment length is 15 bp, so we hoped that this protocol might actually work to open plasmids.
    11 KB (1,718 words) - 21:25, 26 October 2008
  • Team:Hawaii/Notebook/2008-06- 2
    :* Protocol suggests 23C but growth was too slow. Also, protocol is for Mach10 cells, which grows much faster. ... 6 seed stock tubes per [[Team:Hawaii/Make_Competent_E._Coli | compentency protocol]]
    3 KB (445 words) - 02:35, 5 June 2008
  • Team:TUDelft/Meetings
    ...ut the DNA in a gel, got unclear/weird images. It looks like with the IGEM protocol (includes a heating step up to 42oc), you loose DNA but many impurities und *'''PCR''': Ruud is setting up/checking available PCR protocol. 95-50-75 protocol with Taq polymerase seems expectedly working. ''Plans'': Put the genes into
    18 KB (2,754 words) - 09:07, 27 October 2008
  • Team:Hawaii/Biobricks extraction & transformation competency test
    == Protocol == The same protocol as [[Team:Hawaii/Initial_ BioBrick_Plasmid_Extraction_From_Filter_Paper|"In
    3 KB (433 words) - 02:40, 30 October 2008
  • Team:Hawaii/Notebook/2008-06-20
    ===Electroporation Protocol=== :* Posted electroporation protocol. It looks like this will be a viable option for introducing an autonomously
    705 B (98 words) - 06:51, 24 June 2008
  • Team:Heidelberg/Notebook/Killing I/Notebook/week10
    PCR protocol ...anted EcoRI restriction site in cI from geneart was mutated using standard protocol
    35 KB (4,900 words) - 14:09, 29 October 2008
  • Team:MIT/Tooth binding assay protocol
    A couple of things are novel in the protocol. Firstly, we modified the protocol bacteria on the beads (the paper with the original protocol failed to prove
    5 KB (780 words) - 21:12, 29 October 2008
  • Team:MIT/Protein Purification
    ===TEV Purification Protocol=== ===Peptide Purification Protocol===
    2 KB (298 words) - 21:08, 27 June 2008
  • Team:ESBS-Strasbourg/PCR
    -Adapt the protocol function of the mother concentration we have <br> b) Protocol <br>
    3 KB (472 words) - 12:44, 21 July 2008
  • Team:Tsinghua/Notebook
    '''1. Advanced Protocol for Parts Extraction''' We have revised the Parts Extraction Protocol and obtained a higher efficiency of transformation. Here are some details:
    10 KB (1,229 words) - 04:31, 30 October 2008
  • Team:KULeuven/16 July 2008
    ...9]) in competent TOP10 cells using the BioBrick protocol (and not the CMPG protocol). An empty pUC plasmid was used as control. Because the transformation didn ...s punched out and transformed into competent DH5alpha cells using the CMPG protocol, also with empty pUC control.
    1 KB (201 words) - 18:06, 6 October 2008
  • Team:Imperial College/Test Page
    <p class="num">Protocol 1</p> <p class="info">The actual protocol</p>
    5 KB (774 words) - 13:44, 8 September 2008
  • Team:University of Ottawa/6 August 2008
    :<li> Followed miniprep protocol included in kit ...d to be zero. Also, did not mix neutralisation solution as outlined in the protocol.
    5 KB (700 words) - 19:11, 7 August 2008
  • Team:Paris/August 11
    ...g [[Team:Paris/Notebook/Protocols#Transformation|transformation for Top10 protocol]] ==== '''Protocol''' ====
    8 KB (1,065 words) - 16:11, 18 August 2008
  • Team:Paris/August 13
    ...s://2008.igem.org/Team:Paris/Notebook/Protocols#Minipreps_.28Kit_Qiagen.29 Protocol] (see # '''3''') Experiments done by QIAcube * [https://2008.igem.org/Team:Paris/Notebook/Protocols#Glycerol_Stocks Protocol] (see #'''2''')
    8 KB (1,152 words) - 16:12, 15 August 2008
  • Team:Paris/August 19
    [[Team:Paris/Notebook/Protocols#Ligation |Protocol]] [[Team:Paris/Notebook/Protocols#Transformation |Protocol]]
    5 KB (652 words) - 20:11, 4 September 2008
  • Imperial College/31 August 2008
    *Begin to try to optimise the protocol for ''B.subtilis'' transformation protocol with linear DNA. This includes the volume of transformed cells, amount of D *Carry on to optimise the protocol for ''B.subtilis'' transformation protocol with linear DNA
    3 KB (359 words) - 20:42, 28 October 2008
  • Team:Caltech/Project/Vitamins
    ...cols/Folate assay|<font style="color:#BB4400">folate microbiological assay protocol</font>]] page. ...d not show any detectable PABA peaks. Samples were run using the same HPLC protocol as the standards, and both lysate and supernatant were tested for PABA peak
    23 KB (3,375 words) - 18:48, 20 October 2008
  • Imperial College/26 August 2008
    ...MPERIAL/2008/Prototype/Wetlab/Transformation_protocol_4 click here for the protocol], was not performed today. ...equire these midi preps to PCR out biobricks and for use in transformation protocol optimisation.
    2 KB (254 words) - 20:39, 28 October 2008
  • Team:Chiba/jk/β/weak 4
    >[[Team:Chiba/protocol/j|Protocols]] [[Team:Chiba/protocol/phenotype/timedelay/j|time-delay test]]
    2 KB (347 words) - 07:46, 30 September 2008
  • Team:Chiba/protocol/phenotype/timedelay/j
    >[[Team:Chiba/protocol/j|プロトコルページへ]]<Br> === プロトコル Protocol ===
    4 KB (434 words) - 09:28, 15 December 2008
  • Team:Chiba/Calendar-Home/6 September 2008
    '''[[Team:Chiba/protocol/PCR|Colony-PCR]]''' :--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
    4 KB (581 words) - 22:19, 29 October 2008
  • Team:Chiba/Calendar-Home/13 September 2008
    *[[Team:Chiba/protocol/phenotype/timedelay/j|time-delay test]] :'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
    4 KB (634 words) - 23:52, 29 October 2008
  • Team:Chiba/Calendar-Home/16 September 2008
    [[Team:Chiba/protocol/phenotype/timedelay/j|time-delay test]] with (15/9) cultures. [[Team:Chiba/protocol/gelcheck|Gel check]]
    3 KB (429 words) - 00:00, 30 October 2008
  • Team:Chiba/protocol/phenotype/timedelay
    >[[Team:Chiba/protocol|return to protocols page]]<Br> === Protocol ===
    3 KB (504 words) - 08:56, 30 October 2008
  • Team:Imperial College/Transformation 2
    {{Imperial/Box1|Transformation Protocol 2| ======Protocol======
    3 KB (562 words) - 17:39, 28 October 2008
  • Team:Imperial College/Transformation
    {{Imperial/Box1|Transformation Protocol 1| ======Protocol======
    4 KB (624 words) - 11:56, 28 October 2008
  • Team:Imperial College/Growth
    ...low proper characterisation of Promoters and RBSs for ''B.subitlis''. This protocol must give results that are as accurate as possible over a considerable rang ======Protocol======
    2 KB (402 words) - 17:41, 28 October 2008
  • Team:Imperial College/Fluorescence
    *The aim of this protocol is to produce a calibration curve of fluorescence vs molecules of GFPmut3b. ======Protocol======
    3 KB (489 words) - 17:42, 28 October 2008
  • Team:Harvard/Dailybook/Week4/Chemical and Light
    We followed the standard digest protocol under the "General Protocols" section. We used the standard digestion protocol.
    38 KB (5,639 words) - 19:03, 26 October 2008
  • Team:Michigan/Notebook/LigationProtocol
    == <font color=royalblue size=5> Ligation Standard Protocol </font> == =Protocol=
    2 KB (248 words) - 03:06, 30 October 2008
  • Team:Michigan/Notebook/PlasmidPrepProtocol
    == <font color=royalblue size=5> Plasmid Prep Standard Protocol </font> == =Protocol=
    747 B (105 words) - 03:18, 30 October 2008
  • Team:Cambridge/Bacillus subtilis transformation
    ...nfirm our transformations and to be able to standardize our protocol. This protocol is modified from the one provided by the Bacillus Genetic Stock Center (htt ''Protocol of the Amylase Test:''
    7 KB (1,049 words) - 20:39, 29 October 2008
  • Transformation and Plating
    ==Transformation Protocol== '''Heat transformation Protocol'''
    1 KB (233 words) - 04:33, 30 October 2008
  • Re-transform with selected plasmid
    ==Transformation Protocol== '''Heat Transformation Protocol'''
    1 KB (233 words) - 04:33, 30 October 2008
  • Sequence
    ==Sequencing Protocol== '''Protocol:'''
    840 B (111 words) - 01:00, 30 October 2008
  • PCR SOEing
    ==PCR SOEing Protocol== ...along with PCR Supermix and placed into a Thermocycler under the following protocol:
    1 KB (217 words) - 00:58, 30 October 2008
  • Team:Imperial College/Biomaterial Assay
    ======Protocol====== *'''Will finish this tomorrow when I finalise the protocol!!!!!!'''
    3 KB (471 words) - 00:28, 29 October 2008
  • Team:Freiburg Transfection and Synthetic Receptor
    ...f our parts was analyzed in three steps. First, we tested the transfection protocol and checked the utility of the transfection vector CMV construct for expres Transfection was performed with a lac Z gene using the transfection protocol described above. After 48h one part of the cells was harvested by washing t
    7 KB (1,055 words) - 23:04, 29 October 2008
  • TUDelft/16 October 2008
    ...oup). The machine used was a Thermo Savant FastPrep FP120 Homogenizer. The protocol used was:
    988 B (151 words) - 15:33, 27 October 2008
  • October
    The protocol of july 24th was used.<br> Preparation of the ssDNA was done following the QIAPrep Spin M13 Kits protocol. <br>
    45 KB (5,909 words) - 02:40, 30 October 2008
  • SOC
    ==Protocol==
    546 B (73 words) - 02:24, 30 May 2008
  • Team:UNIPV-Pavia/Protocols/PlasmidExtraction
    ...qiagen.com/literature/render.aspx?id=370&tp=4 QIAGEN QIAprep Spin Miniprep protocol]
    2 KB (325 words) - 12:52, 1 July 2008
  • Team:Hawaii/Tri-parental conjugation between E. coli and PCC6803
    == Protocol from Callahan Lab ==
    4 KB (637 words) - 06:56, 27 June 2008
  • Team:Montreal/DNA Agarose Gel
    ==Protocol==
    3 KB (394 words) - 19:57, 17 June 2008
  • Team:Montreal/Cell transformation
    ==Protocol==
    2 KB (247 words) - 19:41, 17 June 2008
  • Team:Montreal/Seeding
    ==Protocol==
    2 KB (239 words) - 19:40, 17 June 2008
  • Team:Hawaii/Protocols/Transformation PCC6803
    == Protocol ==
    3 KB (500 words) - 04:32, 24 June 2008
  • Team:MIT/Modeling
    ...familiarity, we had a hard time growing up the bacteria. We have written a protocol for growing strains of ''Lactobacillus'' [http://openwetware.org/wiki/Lacto ... the ''Lactobacillus''. From our research we found a electrotransformation protocol by Serror et al (App and Env Microbiology, 2002). Further research convince
    6 KB (897 words) - 04:21, 30 October 2008
  • Team:Hawaii/PCC6803 cell density
    == Protocol==
    1 KB (182 words) - 21:53, 27 June 2008
  • Team:University of Lethbridge/Notebook/Project1July
    ...wet ware [http://openwetware.org/wiki/Preparing_chemically_competent_cells protocol]. 100 uL aliquots of cells were frozen in liquid nitrogen and stored in th ...possibility one we will try more DNA. If that does not work we will try a protocol using electroporation.
    14 KB (2,039 words) - 02:32, 30 October 2008
  • Team:Paris/August 4
    ===Protocol of screening PCR===
    2 KB (223 words) - 15:22, 6 August 2008
  • Team:Hawaii/Protocols/oligo hybridization
    ===Protocol===
    637 B (87 words) - 21:59, 9 July 2008
  • Team:Hawaii/Protocols/gel extraction
    ===Protocol===
    1 KB (185 words) - 22:10, 9 July 2008
  • Team:Paris/July 31
    === Protocol ===
    5 KB (791 words) - 17:04, 13 August 2008
  • Team:ESBS-Strasbourg/Transformation Heat shock
    Protocol is a mixture of the IGEM standard protocol and the protocol used in the local lab<br>
    337 B (56 words) - 08:29, 5 August 2008
  • Team:ESBS-Strasbourg/11 July 2008
    ** competent Top10 cells from Invitrogen, protocol from Marias Lab: H2O (negative control), pxj (positive control), 5 BioBrick ** competent db3.1 cells, protocol from Barbaras Lab, DNA was already in KCM and cells were just added and pla
    1 KB (162 words) - 16:53, 16 July 2008
  • Team:ESBS-Strasbourg/Protocols
    ...-Strasbourg/Competent Bacteria | Preparation of competent cells (Barbara's Protocol)]] <br> ...sbourg/Transformation Barbara|Transformation of competent cells (Barbara's Protocol)]]<br>
    2 KB (192 words) - 00:13, 29 October 2008
  • Team:ESBS-Strasbourg/Miniprep: plasmid amplification
    ==b) Protocol ==
    2 KB (262 words) - 09:23, 18 July 2008
  • Team:ESBS-Strasbourg/Restriction digestion
    =b) Protocol = '''double digestion protocol used for plasmid verification 17/07/08 (KK):'''
    2 KB (333 words) - 13:28, 18 July 2008
  • Team:ESBS-Strasbourg/Ligation
    2)Protocol: <br> [http://openwetware.org/wiki/DNA_Ligation see iGEM protocol]
    586 B (91 words) - 08:02, 5 August 2008
  • Team:Hawaii/Biobrick conversions
    ==Protocol==
    9 KB (1,260 words) - 20:53, 15 August 2008
  • Team:TUDelft/25th of September protocol
    =25th of September Luciferase assay protocol= <b>This protocol has been discarded due to interfering of Promega lysis buffer with the prot
    2 KB (257 words) - 13:51, 20 October 2008
  • 15th of October protocol
    =15th of October luciferase assay protocol= <b>This protocol has been discarded due to the inconsistency of sonication amplitude</b>
    2 KB (260 words) - 13:47, 20 October 2008
  • Team:Paris/July 17
    ===Protocol===
    7 KB (1,035 words) - 14:35, 31 July 2008
  • Team:Hawaii/Protocols/ExoSAP
    ==Protocol==
    217 B (37 words) - 23:54, 18 July 2008
  • Team:IIT Madras/Project
    ===Standardized protocol for stress===
    7 KB (1,125 words) - 14:42, 27 October 2008
  • Team:Hawaii/Birds Eye View
    == [[Team:Hawaii/Protocols|Protocols]] ([[Template:Team:Hawaii/Protocol|t]]) ==
    5 KB (626 words) - 03:27, 30 October 2008
  • Team:University of Ottawa/31 July 2008
    :<li> Following protocol outlined online, tested a microwave protocol to hasten digestion period. ...romising; however, more experimentation needed to confirm validity of this protocol
    6 KB (801 words) - 15:05, 12 August 2008
  • Team:Paris/August 5
    === '''PCR Protocol''' ===
    4 KB (533 words) - 15:17, 6 August 2008
  • Team:Paris/July 30
    ===Protocol of screening PCR===
    19 KB (2,548 words) - 17:03, 13 August 2008
  • EPF-Lausanne/7 August 2008
    Ligation protocol : Following the protocol from OpenWetWare, but with our T4 ligase the ligation time is 4 hrs at 15 d
    1 KB (190 words) - 12:11, 7 August 2008
  • My Notes II
    ...t night about what to do with our non-cooperative sca2 and sca3. Got a new protocol for dealing with cotransformed sca2. Jin amplified fimH while he was still *8:45a.m. - miniprepped two of our co-transformed sca2 samples for a new protocol (to rid co-transformation)
    14 KB (2,169 words) - 07:56, 6 September 2008
  • Team:Paris/August 9
    ==='''Protocol of the ligation'''===
    3 KB (363 words) - 17:11, 13 August 2008
  • Imperial College/19 August 2008
    ...ion_Protocol_2| Click link here for protocol]. The basic principle of this protocol is that competent cells are prepared by growing a culture to a high O.D.<su
    1 KB (192 words) - 20:37, 28 October 2008
  • Imperial College/14 August 2008
    ...b/Transformation_protocol_4#Transformation_Protocol_2| Click link here for protocol] *The basic principle of this protocol is that competent cells are prepared by growing a culture to a high O.D.<su
    2 KB (339 words) - 20:36, 28 October 2008
  • Team:Paris/August 14
    ==== Protocol : ==== ...he [[Team:Paris/Notebook/Protocols#Purification_.28Kit_Promega.29|standard protocol]].
    3 KB (343 words) - 14:58, 16 August 2008
  • Team:Chiba/protocol/ligation/gelex
    >[[Team:Chiba/protocol|Protcol]] READ:[[Team:Chiba/protocol/gelcheck|Agarose gel electrophoresis]]
    410 B (64 words) - 03:10, 11 September 2008
  • Team:Caltech/Protocols/Folate assay
    * Previously, we had been using this protocol with the other folate-dependent strain ''Entercoccus hirae'' ATCC 8043, and ...sorbances of the samples at 546 nm on a plate reader (200uL per well). The protocol recommends refrigerating the samples at 4C for 15-30 minutes prior to readi
    6 KB (840 words) - 00:48, 30 October 2008
  • Team:Caltech/Protocols/Coculture Inhibition Assay
    ===Protocol===
    2 KB (233 words) - 01:06, 30 October 2008
  • Team:Paris/August 23
    [[Team:Paris/Notebook/Protocols#Transformation |Protocol]] [[Team:Paris/Notebook/Protocols#Digestion |Protocol]]
    4 KB (610 words) - 14:47, 6 September 2008
  • Team:University of Lethbridge/Notebook/Project2August
    -went over the SELEX protocol with HJ to determine the primers we will need to do this, and how exactly w Same as previous protocol, However, Annealing Temperature is set to 65.0 C
    6 KB (875 words) - 02:34, 30 October 2008
  • Team:Valencia/Project/Lab work/2 experiments
    The objective of this two-step protocol is to be able to start the experiment with a higher O.D. in a medium with l ...to do so, we designed the two inoculum protocol described above. With this protocol, we intend to reach an high O.D. without having too much glucose. We used S
    13 KB (1,943 words) - 00:44, 30 October 2008
  • Imperial College/17 September 2008
    ... link [http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Protocols/Growth#Protocol|here]. The following O.D.<sub>600</sub> and dilutions were used:
    1 KB (178 words) - 20:47, 28 October 2008
  • August
    ...tubes and after 20 minutes I started the transformation with the following protocol<br> There were no colonies on the plates so I changed the protocol and tried it again.<br>
    8 KB (1,289 words) - 02:40, 30 October 2008
  • September
    see at the protocol from 07-24-2008 see at the protocol from 07-24-2008
    8 KB (1,307 words) - 02:41, 30 October 2008
  • Team:Bologna/Biosafety
    ...he need for specific precautions to be adopted during the execution of the protocol. | '''Protocol'''
    29 KB (4,412 words) - 02:19, 30 October 2008
  • 25th of September protocol
    =25th of September Luciferase assay protocol= <b>This protocol has been discarded due to interfering of Promega lysis buffer with the prot
    2 KB (257 words) - 12:18, 20 October 2008
  • Team:Heidelberg/Parts/Characterization
    '''Protocol''' In this experiment we again used the same protocol as described above in test 1 but used different donor / recipient rations,
    87 KB (12,520 words) - 17:05, 29 October 2008
  • Team:Tokyo Tech/Result
    == Protocol ==
    1 KB (193 words) - 13:49, 26 October 2008
  • Team:Chiba/Calendar-Home/15 September 2008
    :'''[[Team:Chiba/protocol/transformation|Transformation]]''' (15/9)-->[[Team:Chiba/protocol/phenotype/T9002/j|T9002 check(AHL sensitivity and time response test)]]
    812 B (101 words) - 00:00, 30 October 2008
  • Team:Chiba/Calendar-Home/26 September 2008
    <br>colony [[Team:Chiba/protocol/PCR|PCR]] <br>->[[Team:Chiba/protocol/gelcheck|Gel check]]
    1 KB (194 words) - 01:08, 30 October 2008
  • IGEM:Cambridge/2008/Turing Pattern Formation/Experiments/Bacillus subtilis transfomation
    ...recise tests confirm our transformations and to be able to standardize our protocol ''Protocol of the amylase test''
    6 KB (969 words) - 22:24, 28 October 2008
  • Team:Chiba/parts/BBa K084008
    ====[[Team:Chiba/protocol/phenotype/timedelay|more details]]====
    2 KB (210 words) - 11:52, 28 October 2008
  • Team:Imperial College/XL1 Blue
    ======Protocol======
    3 KB (419 words) - 17:40, 28 October 2008
  • Team:Harvard/Dailybook/Week11/Chemical and Light
    ** Perhaps ligation protocol is not optimal (use method below) ** Ligations with old protocol failed
    4 KB (647 words) - 19:21, 26 October 2008
  • Team:Heidelberg/Notebook/Killing II/7thweek
    .... Because of that we started a new PCR with the right reverse primers (see protocol at pSB1A3-Receiver cloning) *Transformation of pSB1A3-T9002-without-GFP ON-Ligations (Transformation protocol Chris)
    37 KB (5,261 words) - 21:35, 28 October 2008
  • USTC/Notebook/Top10 chem competent cell transformation
    ===Regular Transformation protocol for Top10/DH5α cells===
    1 KB (201 words) - 09:54, 28 October 2008
  • Team:BrownTwo/Protocols/compcells
    ...ends to be even harder. We have had a great experience with the following protocol from Inoue, getting near lawns of transformants from ligations: Original protocol from Inoue et al.
    5 KB (756 words) - 08:51, 28 October 2008
  • Team:Michigan/Notebook/TransformationProtocol
    == <font color=royalblue size=5> Transformation Standard Protocol </font> ==
    2 KB (239 words) - 03:06, 30 October 2008
  • Team:Freiburg Transfection
    Transfection was performed with a lac z gene using the transfection protocol described above. After 48h one part of the cells was harvested by washing t <h4>'''Testing the transfection protocol'''</h4>
    7 KB (903 words) - 14:30, 29 October 2008
  • Team:Cambridge/Signaling/Experiments
    Again, the last option is most suitable for our investigation. Here's the protocol taken from Balaban et al (http://www.jbc.org/cgi/content/abstract/276/4/265 ***Guangyong Ji at Catholic University has a protocol for purification
    8 KB (1,194 words) - 15:08, 28 October 2008
  • USTC/Notebook/Chem competent cell transformation
    ===Regular Transformation protocol for Top10/DH5α cells===
    1 KB (201 words) - 15:37, 28 October 2008
  • Team:Cambridge/Protocols
    For the protocol see This protocol is designed to purify single- or double-stranded DNA fragments from PCR
    16 KB (2,728 words) - 20:12, 29 October 2008
  • PCR Amplification
    ==PCR Protocol==
    829 B (125 words) - 00:56, 30 October 2008
  • Digestion
    ==Digestion Protocol==
    995 B (165 words) - 00:58, 30 October 2008
  • Restriction Map/ Restriction Digest
    ==Restriction Digest Protocol==
    1 KB (229 words) - 01:00, 30 October 2008
  • CIP Treatment
    ==CIP Treatment Protocol==
    835 B (131 words) - 00:58, 30 October 2008
  • Ligate DNA
    ==Ligation Protocol==
    2 KB (323 words) - 00:59, 30 October 2008
  • Infection
    ==Infection Protocol==
    305 B (48 words) - 01:01, 30 October 2008
  • Lentivirus Production
    ==Virus Production Protocol==
    741 B (121 words) - 01:01, 30 October 2008
  • Lentivirus Harvesting
    ==Virus Harvesting Protocol==
    971 B (141 words) - 01:01, 30 October 2008
  • Transfection (nonviral)
    ==Transfection Protocol==
    609 B (99 words) - 01:02, 30 October 2008
  • Team:Chiba/protocol/ligation/gelex/j
    >[[Team:Chiba/protocol/j|プロトコルページへ]]<Br> これは[[Team:Chiba/protocol/gelcheck/j|電気泳動]]後からの操作です。
    2 KB (65 words) - 05:16, 27 June 2009
  • Team:Hawaii/Milestone
    * Culturing Protocol Optimization: CO2 is bottle neck, need incubator retrofitted (KS) * 2008.06.01 Plasmid Transformation Plan/Protocol (GK/MR)
    2 KB (219 words) - 17:35, 15 August 2008
  • Purdue/23 June 2008
    ...lpha-T1 cells (Invitrogen) and used a combo of their protocol and the iGEM protocol
    2 KB (391 words) - 14:27, 27 June 2008
  • Purdue/8 July 2008
    Yesterday, the two parts were prepped following the iGEM protocol (including the extra step). We used 2 spots of paper, and used 16uL of TE ...s. For the rest of the transformation process, we followed the Invitrogen protocol that is supplied with the cells:
    2 KB (286 words) - 19:06, 8 July 2008
  • Team:Hawaii/Test Competent E. Coli
    ...onential growth of E. coli seed stocks at 23C (Callahan cells, recommended protocol) result in higher competency than growth of E. coli at 37C (our cells)? ... cells were made on 7/11/08 with this [[Team:Hawaii/Make Competent E. Coli|protocol]].
    4 KB (614 words) - 01:33, 16 July 2008
  • Team:Purdue/Project
    ...hat they cannot survive outside of a lab environment. In other words, our protocol was entirely under Biosafety Level I (i.e., little or no health hazard). ...IBC). In cooperation with them, we filled out a form detailing our entire protocol. We then had to indicate any health hazards that could be posed by our pro
    12 KB (1,859 words) - 23:49, 29 October 2008
  • Team:UNIPV-Pavia/Protocols/GelExtraction
    ...-science.com/pack-insert/1696505a.pdf Roche Agarose Gel DNA Extraction Kit protocol]
    2 KB (266 words) - 12:52, 1 July 2008
  • Team:Hawaii/Initial BioBrick Plasmid Extraction From Filter Paper
    ==Protocol== Protocol adapted from Registry of Standard Biological Parts, [http://partsregistry.o
    3 KB (443 words) - 07:18, 12 June 2008
  • Team:Alberta NINT/Protocols
    QIAprep Spin Miniprep Protocol Exracting DNA from gel purified samples - QIAquick gel extraction protocol
    8 KB (1,202 words) - 00:04, 30 October 2008
  • Team:NTU-Singapore/Notebook/14 June 2008
    ...ncentration were pippetted into the respective wells as planned out in the protocol ***The protocol that was used in the previous characterization experiment were used, with a
    2 KB (274 words) - 00:34, 28 October 2008
  • Team:Hawaii/Plasmid Prep
    == Protocol== ...irst two, and 10 of the third because it has a lower copy number. Used the protocol from Dr. Callahan's lab ([[http://packrat.stjohn.hawaii.edu/prestinglab/wik
    4 KB (577 words) - 01:58, 7 August 2008
  • Alberta NINT/16 June 2008
    ...(I732018) from 15/06/08 LB + amp culture tubes using QIAprep spin miniprep protocol, Extracted DNA from gel usig QIAquick gel extraction protocol.
    1 KB (175 words) - 22:50, 11 October 2008
  • Team:Hawaii/Notebook/2008-06-17
    :* Same protocol as 6/3/08 competency test :* Extracted BBa_B0024, BBa_B0025, and BBa_E0240 from filter paper using same protocol 6/9/08
    1 KB (183 words) - 04:31, 18 June 2008
  • Team:Hawaii/PCC6803 Electroporation of PCC6803
    * This protocol is adapted from a protocol for PCC6714, which is not naturally competent. However it seems we can use
    3 KB (464 words) - 18:33, 30 June 2008
  • Team:University of Chicago/Notebook/Norayucel
    #Final OD after following competent cell protocol was 1.04 (between 1-1.5 as required) *Prepped using Thomas’s protocol
    11 KB (1,571 words) - 16:42, 28 September 2008
  • Minnesota/19 June 2008
    |5. '''Sequencing Protocol:''' John Barrett demonstrated the sequencing protocol which we will be using in the future. The first step is to determine the DN
    5 KB (703 words) - 15:48, 2 July 2008
  • Team:University of Washington/Protocols
    ''modified from the preceding protocol 07-25'' ''Modified from Endy protocol specifically for AHL cloning 07-26''
    12 KB (1,822 words) - 21:31, 22 January 2009
  • Team:Johns Hopkins/Notebook
    July 11: Lab meeting at 7:30PM in the lab to go over miniprep protocol. in protocol however, so final transfer to the iGEM vector should still be straightforwa
    11 KB (1,649 words) - 04:50, 30 October 2008
  • Team:NTU-Singapore/Parts/Characterization of LacI-GFP
    ...on experiment, please click on the file: [[Image:NTU@iGEM Characterization Protocol.pdf]]<br>
    10 KB (1,474 words) - 05:49, 27 October 2008
  • Team:Hawaii/PCR Amplification of pRL1383a
    MinElute PCR Purification Kit Protocol from Qiagen This protocol is designed to purify double-stranded DNA fragments from PCR reactions
    22 KB (3,349 words) - 00:48, 30 October 2008
  • Team:NTU-Singapore/Notebook/9 July 2008
    **Protocol different from 8 July *** Optimize Protocol
    4 KB (583 words) - 02:25, 28 October 2008
  • Team:Hawaii/Procols/DNA ligation
    ===Protocol=== ...:1 ratio of insert to vector. ~180ng of DNA can be ligated using the above protocol.
    901 B (146 words) - 05:31, 13 August 2008
  • Team:University of Ottawa/10 July 2008
    ::<li> encountered issues with protocol and communication; standard ligation not exactly followed ::<li> followed Phusion high fidelity PCR protocol
    6 KB (863 words) - 18:32, 30 July 2008
  • Team:EPF-Lausanne/Project
    ...ntrol on the signaling is equivalent to choosing a cell-cell communication protocol. ...e therefore exploited this feature to characterize the respective part(see protocol in parts registry BBa_K092600). We found that RPF was indeed produced thus
    16 KB (2,582 words) - 23:12, 29 October 2008
  • Team:Hawaii/Notebook/2008-07-22
    ...irst two, and 10 of the third because it has a lower copy number. Used the protocol from Dr. Callahan's lab ([[http://packrat.stjohn.hawaii.edu/prestinglab/wik ...the aadA region using the "Isopropanol Precipitation for PCR purification" protocol from openwetware.org
    3 KB (438 words) - 00:40, 26 July 2008
  • Team:ESBS-Strasbourg/Purification of PCR product
    b) Protocol <br> b) Protocol <br>
    2 KB (246 words) - 13:48, 14 July 2008
  • Team:Hawaii/Protocols/Colony PCR
    ==Protocol== Protocol dictated by [http://www2.hawaii.edu/~scallaha/SMCsite/ Dr. Sean Callahan],
    837 B (123 words) - 08:10, 16 July 2008
  • Team:TUDelft/15th of October protocol
    =15th of October luciferase assay protocol= **There is a standard protocol on the computer in which you only have to indicate the wells to be assayed.
    2 KB (246 words) - 14:39, 29 October 2008
  • Team:ESBS-Strasbourg/4 August 2008
    * ligation into the XbaI, PstI cutted vector pSB1A2 ''(using protocol 2c)'' ...rmation into competent TOP10 and plating on LB-Amp ''(using the heat-shock protocol)''
    497 B (72 words) - 16:08, 6 August 2008
  • Team:Chiba/jk/β
    [[Team:Chiba/protocol/phenotype/timedelay/j|time-delay protocol]]
    5 KB (356 words) - 11:27, 14 December 2008
  • Team:University of Ottawa/Transformation of Competent Ecoli
    ...he workbench should be accompanied by a flame for sterility. An alternate protocol that includes a heat shock treatment is also provided, but has not been sho
    6 KB (986 words) - 22:48, 27 October 2008
  • Team:Paris/July 26
    * Use of Promega's protocol on all the clones cultivated on the 25th. * Use of Promega's protocol for the extraction.
    15 KB (1,962 words) - 16:55, 31 July 2008
  • EPF-Lausanne/28 July 2008
    ...one, using pUC as well as 1000 2C (something useless to us), with the same protocol as before. Our cells are probably not competent. We also transform with heat shock normal protocol our Top10 cells with the purified stock of what we received from iGEM last
    1,005 B (169 words) - 12:13, 7 August 2008
  • Team:KULeuven/28 July 2008
    ...ransformed into competent TOP10 cells using the iGEM protocol and the CMPG protocol.
    1 KB (234 words) - 13:08, 7 October 2008
  • Alberta NINT/29 July 2008
    miniprep protocol, using 5 ml of culture and a minelute spin column, eluting in 15 ul of Buff Extracted DNA from gel usig QIAquick gel extraction protocol.
    1 KB (151 words) - 23:04, 11 October 2008
  • Imperial College/4 September 2008
    ...-Blue_preparation the relevant protocol] listed on our Protocols Page. The protocol was updated to include a higher innoculation value from the overnight cultu
    3 KB (431 words) - 20:44, 28 October 2008
  • Team:Paris/August 24
    [[Team:Paris/Notebook/Protocols#Migration after digestion| Protocol]] [[Team:Paris/Notebook/Protocols#Ligation |Protocol]]
    2 KB (228 words) - 12:58, 25 August 2008
  • TUDelft/18 August 2008
    ... soaked from the spots. To test the soaking-protocol, various steps of the protocol were performed on the DNA. Either just soaking at Tr or 20' at 42 C, and on
    2 KB (366 words) - 08:39, 27 October 2008
  • TUDelft/22 August 2008
    ... soaked from the spots. To test the soaking-protocol, various steps of the protocol were performed on the DNA. This time 10 ul of TE was used, because due to t
    3 KB (408 words) - 08:52, 27 October 2008
  • Imperial College/15 August 2008
    ...nsformation_protocol_4#Transformation_Protocol_2| (click link here for the protocol)] was performed yesterday. The transformed cells have not yet grown on the
    1 KB (129 words) - 20:36, 28 October 2008
  • Team:Chiba/Calendar-Home/17 August 2008
    :'''[[Team:Chiba/protocol/transformation|Transformation]]''' '''[[Team:Chiba/protocol/PCR|PCR]]'''
    3 KB (411 words) - 05:11, 30 October 2008
  • Alberta NINT/18 August 2008
    ...A3.1 and pSB1A3.3 from the gel from 15/08/08 using QIAquick gel extraction protocol. ....1, TA2.7, TA3.1, TA3.2, TA6.1 and TA7.2 again using an adapted sequencing protocol.
    1 KB (156 words) - 23:09, 11 October 2008
  • Team:Paris/September 4
    [[Team:Paris/Notebook/Protocols#Concentration_of_the_Miniprep | Protocol (it's same that for Miniprep)]] [[Team:Paris/Notebook/Protocols#Ligation |Protocol]]
    3 KB (392 words) - 19:15, 16 September 2008
  • Newcastle University Wetlab/12 September 2008
    * Plasmid was isolated from the 12 pGFPrrnB-BB107 ON cultures 9see protocol). Unfortunately plasmid from colony 4 (25μl plate) and colony 4 (250μl pl ...ncl08 colony 7 and pJWV021 colony 13 isolated from overnight cultures (see protocol).
    2 KB (261 words) - 18:59, 21 September 2008
  • Team:Paris/September 1
    [[Team:Paris/Notebook/Protocols#Concentration_of_the_Miniprep | Protocol (it's same that for Miniprep)]] [[Team:Paris/Notebook/Protocols#Ligation |Protocol]]
    1 KB (177 words) - 14:49, 13 September 2008
  • Newcastle University Wetlab/8 September 2008
    * The ''B. subtilis'' cells were prepared for microscopy (see protocol) and viewed: ...rch, sucrose and glucose [[Making Agar Plates|agar]] plates were made (see protocol) and the following colonies streaked:
    3 KB (487 words) - 16:41, 28 October 2008
  • Team:Chiba/protocol/gelcheck
    >[[Team:Chiba/protocol|Protocol]]
    820 B (132 words) - 02:43, 11 September 2008
  • Team:Chiba/protocol/ligation/dephosphorylation
    >[[Team:Chiba/protocol|Protcol]] READ:[[Team:Chiba/protocol/DNA_Purification/zymocleam|Zymo cleam]]
    452 B (59 words) - 03:09, 11 September 2008
  • Imperial College/20 August 2008
    ...b/Transformation_protocol_4#Transformation_Protocol_2| Click link here for protocol] was carried out on competent cells that had been grown to an O.D.<sub>600<
    2 KB (337 words) - 20:38, 28 October 2008
  • /Project/Vitamins
    [[Folate microbiological assay protocol]] [[para-aminobenzoic acid (pABA) HPLC protocol]]
    10 KB (1,460 words) - 17:32, 22 August 2008
  • Purdue/16 October 2008
    Performed another ligation (for protocol, see Sept. 25). ...01 cells (which naturally take up plasmids). We are following the Bio-Rad protocol for transformation:
    894 B (142 words) - 15:05, 16 October 2008
  • Team:Caltech/Protocols/PABA HPLC assay
    ...e="verdana" style="color:#CC3300">para-Aminobenzoic Acid (pABA) HPLC Assay Protocol</font></div> ...ns starting from 10ug/ml pABA in wild type bacterial lysate using the same protocol to generate a linear standard (see below).
    2 KB (351 words) - 20:55, 15 October 2008
  • Team:Caltech/Protocols
    **[[Team:Caltech/Protocols/Folate_assay|Folate microbiological assay protocol]] ...Team:Caltech/Protocols/PABA_HPLC_assay| para-Aminobenzoic Acid (pABA) HPLC protocol]]
    1 KB (140 words) - 01:04, 30 October 2008
  • Team:Chiba/protocol/transformation
    >[[Team:Chiba/protocol|Protocol]]
    905 B (141 words) - 03:34, 18 September 2008
  • Imperial College/22 August 2008
    ...008/Prototype/Wetlab/Transformation_protocol_2| click here for link to the protocol], was checked today. The plate containing ''B.subtilis'' transformed with n
    1 KB (138 words) - 20:39, 28 October 2008
  • Imperial College/24 August 2008
    ...MPERIAL/2008/Prototype/Wetlab/Transformation_protocol_4 click here for the protocol], this time use 500ul of competent cells as opposed to 200ul as we have pre ...10 vector by cutting with suitable enzymes. We will use the transformation protocol 1.
    2 KB (273 words) - 20:39, 28 October 2008
  • Team:ETH Zurich/Wet Lab/Materials
    ...<td><table><tr><td width=5px></td><td>[[Team:ETH_Zurich/Wet%20Lab/Protocol|Protocol]]</td></tr><tr><td width=5px></td><td>[[Team:ETH_Zurich/Wet%20Lab/Materials
    2 KB (287 words) - 15:50, 7 September 2008
  • Transforming into DH5α or TOP10 E. coli
    When transforming into DH5-alpha E.coli cells we followed the protocol below: * On ice, add 12.5µl of the ligation mixture (see [[Ligating DNA]] protocol) to 100µl of DH5-alpha E.coli cells in an eppendorf tube
    969 B (154 words) - 16:06, 28 October 2008
  • Transformations
    When transforming into DH5-alpha ''E.coli'' cells we followed the protocol below: * On ice, add 12.5µl of the ligation mixture (see Ligating DNA protocol) to 100µl of DH5-alpha E.coli cells in an eppendorf tube
    885 B (145 words) - 12:03, 18 September 2008
  • Team:Chiba/jk/β/week 6
    >[[Team:Chiba/protocol/j|Protocols]] *[[Team:Chiba/protocol/phenotype/timedelay/j|time-delay test]]
    1 KB (137 words) - 12:11, 30 September 2008
  • Team:LCG-UNAM-Mexico/Notebook/2008-September
    <p>We Cloned the RcnA PCR fragment according to the ColoneJet protocol of the  <strong>CloneJET PCR cloning Kit by Fermentas,</strong> and let t <p>We Cloned the RcnA PCR fragment according to the ColoneJet protocol of the  <strong>CloneJET PCR cloning Kit by Fermentas,</strong> and let t
    42 KB (6,194 words) - 02:06, 30 October 2008
  • Team:Heidelberg/7 October 2008
    ...added a GFP gene for visualizing and measurement purposes and an OriT (see protocol 2). To be able to later modify this phage insert again, we added special re The parts in pBluescript are functional (for conjugation results see protocol 2).
    9 KB (1,534 words) - 15:27, 8 October 2008
  • Team:NTU-Singapore/Parts/Standard Promoters
    ...for our own characterization procedure. We have decided to make use of the protocol given to us, however with some minor modifications. ...e parts transformed, we can proceed with the characterization based on the protocol we constructed. We make use of the FLx800™ Fluorescence Microplate Reader
    5 KB (700 words) - 05:48, 27 October 2008
  • Team:ETH Zurich/Wetlab/Switch Circuit
    ...oil.com Quantifoil]) and stained with uranyl acetate according to standard protocol (1min sample adsorption, three times washing with 1% uranyl acetate solutio ...s performed using vector pET28a-RMF as template according to the following protocol: Each reaction setup contained 35ul water, 10ul [http://www.finnzymes.com/h
    46 KB (7,198 words) - 03:55, 30 October 2008
  • Team:NTU-Singapore/Parts/iGEM Standardisation
    ...pproach our characterization procedure. We have decided to make use of the protocol given to us, however with some minor modifications. ...e parts transformed, we can proceed with the characterization based on the protocol we constructed. We make use of the FLx800™ Fluorescence Microplate Reader
    2 KB (375 words) - 00:32, 24 October 2008
  • TUDelft/21 October 2008
    A new protocol for cell lysis was tested: after 1ml of o/n LB culture (OD=~1) is resuspend ...6 were grown o/n @ 30ºC. These tubes will be used to test the Bead Beater protocol properly.
    2 KB (278 words) - 12:37, 29 October 2008
  • Team:Chiba/Calendar-Home/19 August 2008
    :'''[[Team:Chiba/protocol/PCR|PCR]]''' :--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
    2 KB (332 words) - 05:55, 30 October 2008
  • TUDelft/22 October 2008
    We've done some luciferase measurements also using the old protocol with lysis buffer. These are displayed in figure 3, but '''aren't corrected ...fferent vectors were prepped with a Qiagen kit according to manufacturer's protocol. These three didn't yield a sufficient DNA concentration yesterday. Today a
    1 KB (180 words) - 14:03, 28 October 2008
  • Team:Heidelberg/Notebook/material
    ...g out the mixture in 3 ml molten top agarose. Agarose is preferred in this protocol in order to minimize contaminations with polyanionic inhibitors that can in This protocol for the functional check AI-2 production is mainly adapted from a paper of
    69 KB (9,346 words) - 09:15, 20 August 2009
  • Meetings
    * protocol/write labjournal on wiki <p><b><font face="Arial">6. Transfection Protocol</font></b></p>
    24 KB (3,812 words) - 01:30, 20 November 2008
  • Team:Chiba/Calendar-Home/5 September 2008
    [[Team:Chiba/protocol/digestion|Digestion test]] [[Team:Chiba/protocol/gelcheck|Gel Check]]
    6 KB (1,043 words) - 21:06, 29 October 2008
  • Team:University of Sheffield/Eva
    **Protocol used for characterization : Even repeating the protocol for 5 times the results were consistent.
    8 KB (1,157 words) - 11:48, 29 October 2008
  • Team:Heidelberg/Notebook/Killing I/Notebook/week4
    PCR protocol * digestion of lambda-DNA with XhoI-XbaI following the old protocol
    29 KB (4,122 words) - 11:49, 29 October 2008
  • Team:Heidelberg/Notebook/Killing I/Notebook/week8
    * digestion of the lambda phage with XbaI, XhoI (protocol described previously) PCR protocol
    27 KB (3,801 words) - 11:47, 29 October 2008
  • Team:Harvard/Dailybook/Week1
    * knockout protocol * create basic MFC protocol
    816 B (101 words) - 18:53, 26 October 2008
  • Team:Harvard/Dailybook/Week1/Chemical and Light
    ....org/wiki/IGEM:Harvard/2008/Lab_Notebooks/GenProtocols#Colony_PCR_protocol protocol] listed in General Protocols. The protocol for getting DNA from the 2007 registry is as follows:
    34 KB (5,237 words) - 18:54, 26 October 2008
  • EPF-Lausanne/20 October 2008
    **Using the simplified ligation protocol. **Here a modified version of the ligation protocol was used, because the insert is quite big (over 2kb).
    595 B (86 words) - 09:05, 29 October 2008
  • TUDelft/24 October 2008
    ...n H<sub>2</sub>O) were resuspended in 1x lysis buffer. After precipitation protocol and BCA assay was performed, standard calibration curves were made for all ...precipitation was conducted. R<sup>2</sup> values are depicted next to the protocol name in the legend. Results shown are averages of three seperate measuremen
    2 KB (340 words) - 21:04, 29 October 2008
  • Team:Slovenia/Notebook/Methods
    ...text-align: justify;"><span style="color: rgb(0, 0, 0);"><b>FLOW CYTOMETRY PROTOCOL FOR INTRACELLULAR STAINING OF HEK293T</b></span></p> ...text-align: justify;"><span style="color: rgb(0, 0, 0);"><b>FLOW CYTOMETRY PROTOCOL FOR SURFACE STAINING OF HEK293T&nbsp;</b></span></p>
    48 KB (6,976 words) - 04:42, 30 October 2008
  • Team:University of Sheffield /Timetable
    ...he Univeristy of Sheffield Medical School provided us with electroporation protocol. From 11th August to mid September the electroporation was carried out unsu ...ucing CAI -1 (Cholera Autoinducer – 1) with the appropriate purification protocol.
    7 KB (1,025 words) - 11:58, 28 October 2008
  • Team:Edinburgh/Results/Bacillobricks
    This was then stored as '''Bacillobrick linear vector 1'''. The following protocol was then used to generate linear BioBrick<sup>TM</sup>-containing DNA for t Initially we tested this protocol using BBa_J33204 which carries the promoterless reporter gene ''xylE'', as
    12 KB (1,964 words) - 02:11, 30 October 2008
  • Team:UC Berkeley/GatewayGenomic
    ...sis device into our new entry vector in order to simplify the experimental protocol for this scheme by eliminating mini-preps. ...t eliminates unwanted background by using positive selection. However, the protocol for this scheme still requires that the plasmid mixture is transformed into
    6 KB (905 words) - 04:14, 30 October 2008
  • /Validation
    Volocity 64-Bit was used to analyse synthetic videos created. The following protocol was followed: ...ls were tracked using ImageJ via the Manual Tracking Plugin. The following protocol was followed:
    4 KB (606 words) - 17:38, 28 October 2008
  • Team:Imperial College/Validation
    Volocity 64-Bit was used to analyse synthetic videos created. The following protocol was followed: ...ls were tracked using ImageJ via the Manual Tracking Plugin. The following protocol was followed:
    5 KB (751 words) - 02:56, 30 October 2008
  • IGEM:Cambridge/2008/Notebook/Voltage/BioBrick Manipulation
    ...lates at 37°C and single colonies were then PCRed using standard BioBrick protocol. ...lates at 37°C and single colonies were then PCRed using standard BioBrick protocol.
    4 KB (608 words) - 22:29, 29 October 2008
  • Team:Heidelberg/Notebook/Visualization/Methods
    PROTOCOL FOR SWARM-PLATE CHEMOTAXIS AGAINST A POSSIBLE ATTRACTANT:<br> This protocol was created using petri-dishes. But every other chamber could be used as we
    31 KB (4,573 words) - 19:38, 29 October 2008
  • Team:University of Sheffield/Gosia
    The procedure was carried out in accordance with Protocol [X] except that 1 µl of 100 mM stock solutions of forward and reverse prim ...sured with spectrophotometer. Restriction digest was carried out as in the Protocol [X]. Genes were restriction digested with enzymes SpeI and EcoRI from NEB
    6 KB (998 words) - 12:41, 29 October 2008
  • Team:Heidelberg/Notebook/Visualization/Notebook/SwarmAssays
    So up to now a general protocol with which further swarm assys can be created step by step has been establi The latest protocol can be viewed under the topic of swarm assays on [https://2008.igem.org/Tea
    24 KB (3,335 words) - 19:48, 29 October 2008
  • Team:Edinburgh/Protocols/Bacillobricks
    This was then stored as '''Bacillobrick linear vector 1'''. The following protocol was then used to generate linear BioBrick<sup>TM</sup>-containing DNA for t Initially we tested this protocol using BBa_J33204 which carries the promoterless reporter gene ''xylE'', as
    12 KB (1,964 words) - 02:21, 30 October 2008
  • Team:University of Ottawa
    var gaJsHost = (("https:" == document.location.protocol) ? "https://ssl." : "http://www.");
    6 KB (886 words) - 22:17, 28 October 2008
  • Team:University of Ottawa/Team
    var gaJsHost = (("https:" == document.location.protocol) ? "https://ssl." : "http://www.");
    15 KB (2,160 words) - 01:26, 29 October 2008
  • Team:Montreal/Team
    ...ch bent. As lab supervisor, he is concerned with proper observation of lab protocol and ready availability of materials (without resorting to plunder from the
    6 KB (939 words) - 17:50, 30 May 2018
  • Team:University of Ottawa/Project
    var gaJsHost = (("https:" == document.location.protocol) ? "https://ssl." : "http://www.");
    22 KB (3,281 words) - 00:50, 30 October 2008
  • Team:University of Ottawa/Parts
    var gaJsHost = (("https:" == document.location.protocol) ? "https://ssl." : "http://www.");
    5 KB (781 words) - 19:24, 27 October 2008
  • Team:University of Ottawa/Modeling
    var gaJsHost = (("https:" == document.location.protocol) ? "https://ssl." : "http://www.");
    22 KB (3,330 words) - 00:58, 30 October 2008
  • Team:University of Ottawa/Notebook
    var gaJsHost = (("https:" == document.location.protocol) ? "https://ssl." : "http://www.");
    6 KB (826 words) - 22:12, 28 October 2008
  • Team:The University of Alberta/21 May 2008
    <li>Followed standard Qiagen miniprep protocol for all o/n's.</ul><br>
    2 KB (420 words) - 22:32, 23 May 2008
  • Team:Purdue/Notebook
    '''September 2''' Digestion protocol
    2 KB (248 words) - 23:29, 29 October 2008
  • Team:Hawaii/Project
    ... characterize the plasmids we have produced. We also need to work out the protocol for blue-white selection in DH5&alpha; using K125000. Though the correct B
    7 KB (1,011 words) - 03:46, 30 October 2008
  • Team:Hawaii/Brainstorming
    var gaJsHost = (("https:" == document.location.protocol) ? "https://ssl." : "http://www.");
    1 KB (167 words) - 07:00, 26 May 2008
  • Team:Hawaii/Notebook/2008-05-23
    var gaJsHost = (("https:" == document.location.protocol) ? "https://ssl." : "http://www.");
    1 KB (160 words) - 01:23, 12 June 2008
  • Team:University of Chicago/Meetings
    -Come up with cloning protocol
    11 KB (1,828 words) - 00:23, 22 July 2008
  • Team:NTU-Singapore/Project
    The system will be developed using the following proposed protocol: Colicin E7 production is achieved by the insertion of the colicin E7 gene
    5 KB (768 words) - 14:55, 28 October 2008
  • Team:The University of Alberta/26 May 2008
    ...mply no way of following otherwise. A simple summary of each procedure and protocol would be GREATLY appreciated!
    5 KB (828 words) - 16:26, 27 May 2008
  • Team:Hawaii/Notebook/2008-05-24
    var gaJsHost = (("https:" == document.location.protocol) ? "https://ssl." : "http://www.");
    3 KB (398 words) - 01:28, 12 June 2008
  • Alberta NINT/14 May 2008
    ... Isolated plasmid DNA from culture tubes using QIAprep miniprep standard protocol.
    511 B (63 words) - 21:41, 11 October 2008
  • Alberta NINT/20 May 2008
    protocol.
    353 B (45 words) - 21:40, 11 October 2008
  • Alberta NINT/23 May 2008
    ...id DNA from LB culture tubes from 22/05/08 using QIAprep standard miniprep protocol.
    398 B (45 words) - 21:39, 11 October 2008
  • Team:University of Alberta/Plant Project
    [[Transformation Protocol]]
    4 KB (541 words) - 23:25, 29 October 2008
  • Illinois/27 May 2008
    **Transduction protocol
    2 KB (360 words) - 16:14, 2 June 2008
  • Purdue/3 June 2008
    Protocol for taking measurements followed from June 2, 2008 page.
    3 KB (379 words) - 21:31, 3 June 2008
  • Purdue/25 June 2008
    Used a slightly different protocol, at Jessamine's suggestion:
    1 KB (250 words) - 14:22, 27 June 2008
  • Purdue/14 July 2008
    ...biotic media, but couldn't get the bottle open). The stock from the Clase protocol was a more normal gel, while the iGEM stock took some time to melt and was
    604 B (103 words) - 17:42, 14 July 2008
  • Purdue/15 July 2008
    *Today made a glycerol stock of the GFP E. coli following Clase's protocol
    313 B (49 words) - 16:39, 16 July 2008
  • Team:Illinois/Protocols
    [http://www.protocol-online.org/prot/Model_Organisms/Yeast/Yeast_Culture/ Longterm storage and s [http://www.protocol-online.org/prot/Model_Organisms/Yeast/Yeast_Transformation/ Yeast transform
    6 KB (865 words) - 02:03, 8 October 2008
  • Team:BCCS-Bristol/Misc
    * '''[http://www.protocol-online.org Protocol Online]''' - Lab protocols for various areas of biology.
    2 KB (265 words) - 14:53, 28 October 2008
  • Alberta NINT/30 May 2008
    protocol.
    779 B (101 words) - 22:12, 11 October 2008
  • Team:Hawaii/Notebook/2008-06- 1
    * Designed PCR protocol
    320 B (45 words) - 04:39, 3 June 2008
  • Team:Hawaii/Meeting/2008-06-26
    ##* Primer Design notes (subtle variations & Hawaii protocol)
    3 KB (431 words) - 02:35, 28 June 2008
  • Team:Hawaii/Notebook/2008-06-19
    :* Plasmid prep was performed according to the experimental protocol
    1 KB (180 words) - 03:50, 26 June 2008
  • Team:TUDelft/Temperature testing
    The protocol for the validation will be as follows:
    4 KB (520 words) - 20:36, 29 October 2008
  • Alberta NINT/3 June 2008
    Used QIAquick nucleotide removal standard protocol to purify T/A 0.
    533 B (72 words) - 22:16, 11 October 2008
  • TUDelft/13 October 2008
    ...cribed in the [https://2008.igem.org/Team:TUDelft/15th_of_October_protocol protocol]. Sonication amplitudes varied from 8 microns to 10 microns.
    757 B (102 words) - 14:45, 27 October 2008
  • TUDelft/14 October 2008
    ...cribed in the [https://2008.igem.org/Team:TUDelft/15th_of_October_protocol protocol]. Sonication amplitudes varied from 8 microns to 4 microns at 37ºC culture
    464 B (61 words) - 15:09, 27 October 2008
  • Team:UC Berkeley Tools/Project
    ...(Latency, Throughput, etc)'''. Behavior defines how a device operates (bus protocol for example). Performance is a cost of that behavior (bus transaction laten
    45 KB (7,036 words) - 03:39, 30 October 2008
  • Illinois/3 June 2008
    ***find exact protocol for binding a protein to a vesicle
    1 KB (184 words) - 17:14, 9 June 2008
  • Team:Hawaii/Notebook/2008-06- 4
    :* made Kanamycin and Ampicillin LB plates according to Drew Endy's Protocol [http://www.openwetware.org/wiki/Endy:Pouring_LB_plates_from_prepped_media
    1 KB (165 words) - 03:28, 12 June 2008
  • Alberta NINT/6 June 2008
    ...A from LB + amp50 culture tubes (05/06/08) using QIAprep standard miniprep protocol.
    1 KB (123 words) - 22:41, 11 October 2008
  • Team:Hawaii/Notebook/2008-06- 5
    :* Used same protocol as competency test on 6/3/08
    923 B (132 words) - 03:27, 12 June 2008
  • Team:The University of Alberta/6 June 2008
    ...or Purple Russian and Tryp that we got last week (using the PFU polymerase protocol).
    2 KB (336 words) - 22:56, 6 June 2008
  • Alberta NINT/5 June 2008
    ...est using NEB 2 buffer. Purified using MinElute PCR purification standard protocol.
    2 KB (269 words) - 22:38, 11 October 2008
  • Team:Hawaii/PCC6803 Cryostock Thaw Test
    * Thaw PCC6803 -80C deep frozen stock to ensure that our cryogenic protocol works. This will ensure that we will have re animatable Synechocystis cell
    2 KB (369 words) - 06:54, 12 June 2008
  • Team:UNIPV-Pavia/Notebook/Week4
    *We performed digestion protocol to open plasmids:
    9 KB (1,282 words) - 21:25, 26 October 2008
  • Team:University of Ottawa/25 June 2008
    ::<li> I decided to use the Kaern Lab protocol for PCR Amplification.
    5 KB (804 words) - 23:38, 28 October 2008
  • Team:Hawaii/Notebook/2008-06-10
    :* Got ''Anabena'' triparental conjugation protocol from SC
    2 KB (225 words) - 01:06, 12 June 2008
  • Team:Hawaii/Meeting/2008-06-12
    :* Team: find electroporation protocol for PCC6803 to decrease transformation time. Try it.
    3 KB (536 words) - 21:46, 12 June 2008
  • Team:University of Ottawa/10 June 2008
    ::<li> Cory made a protocol for us to follow for performing PCR.
    4 KB (557 words) - 22:39, 28 October 2008
  • Team:Illinois/Current Tasks
    [http://www.protocol-online.org/prot/Model_Organisms/Yeast/Yeast_Culture/ Longterm storage and s [http://www.protocol-online.org/prot/Model_Organisms/Yeast/Yeast_Transformation/ Yeast transform
    6 KB (892 words) - 02:00, 29 October 2008
  • Alberta NINT/12 June 2008
    ... Isolated DNA from 11/06/08 overnights using QIAprep standard miniprep protocol.
    1 KB (134 words) - 22:48, 11 October 2008
  • Alberta NINT/13 June 2008
    cultures, DNA was isolated by QIAprep standard miniprep protocol.
    826 B (107 words) - 22:49, 11 October 2008
  • Team:Hawaii/Notebook/2008-06-14
    :* Same protocol as 6/2 competent cells
    1 KB (191 words) - 00:56, 15 June 2008
  • Team:NTU-Singapore/Notebook/13 June 2008
    ...e student, Zhang Zhengwen, who adviced on some slight modifications to the protocol set for the characterization experiment
    1 KB (194 words) - 00:34, 28 October 2008
  • Team:Newcastle University/Notebook
    ...orrect plasmids to (self made) competent ''Bacillus'', as described in the protocol section. For plasmid pGFP-ncl08 and pGFP-agr, bacillus was plated on chlora
    13 KB (1,919 words) - 01:53, 30 October 2008
  • Team:Minnesota/Project
    '''Protocol Papers'''
    10 KB (1,668 words) - 15:22, 27 June 2008
  • Team:Rice University/Electroporation
    ... the prep, or the electroporation cuvette was bad, and the electroporation protocol needs to be redone.
    3 KB (443 words) - 18:56, 18 June 2008
  • Team:Rice University/Notebook/18 June 2008
    ***Zymo DNA Recovery protocol
    453 B (76 words) - 20:02, 18 June 2008
  • Team:Rice University/Notebook/19 June 2008
    ***Zymo DNA Recovery protocol
    322 B (51 words) - 18:00, 19 June 2008
  • Alberta NINT/18 June 2008
    ... For Wayne: Extracted K102011/H+X from gel using QIA quick gel extraction protocol and a min-elute
    2 KB (208 words) - 22:51, 11 October 2008
  • Alberta NINT/19 June 2008
    ... (K102002) from LB culture tubes from 18/06/08 using QIAprep spin miniprep protocol.
    799 B (94 words) - 22:52, 11 October 2008
  • Alberta NINT/20 June 2008
    Purified TA0 using QIAquick nucleotide removal protocol.
    798 B (103 words) - 22:52, 11 October 2008
  • Team:MIT/Notebook
    ...Team:MIT/Transforming_Lactococcus lactis|Lactococcus lactis Transformation Protocol]]
    5 KB (605 words) - 14:25, 29 October 2008
  • Team:Edinburgh/Results/Edinbrick1
    ...ing of PCR products in pSB1A2 to make BioBricks<sup>TM</sup>. The standard protocol we use is as follows:
    3 KB (529 words) - 02:03, 30 October 2008
  • Team:The University of Alberta/23 June 2008
    ...etty much exactly 6 weeks from when we planted them. This means our growth protocol works well.
    1 KB (195 words) - 21:44, 26 June 2008
  • Team:Hawaii/Protocols/Transformation E coli
    == Protocol ==
    586 B (91 words) - 05:34, 13 August 2008
  • Brainstorming
    * S. mutans and handling protocol (Chia is contacting Daniel Smith and Martin Taubman of the Forsyth Institut
    5 KB (783 words) - 16:19, 24 June 2008
  • Daily Notebook
    * [[../toothbindingassay | Tooth binding assay protocol]]
    4 KB (634 words) - 16:30, 24 June 2008
  • Organizational Daily Notebook
    * [[../toothbindingassay | Tooth binding assay protocol]]
    5 KB (713 words) - 18:45, 25 June 2008
  • BRAINSTORMING
    * S. mutans and handling protocol (Chia is contacting Daniel Smith and Martin Taubman of the Forsyth Institut
    5 KB (782 words) - 17:16, 24 June 2008
  • Team:Hawaii/Protocols/Cryopreserve cells
    .../IGEM:Harvard/2006/Cyanobacteria/Protocols Harvard iGEM 2006 Cyanobacteria Protocol]
    3 KB (365 words) - 22:48, 24 June 2008
  • Team:Chiba/Internal
    :*'''[[Team:Chiba/protocol/j|実験操作]]（NEW！）'''
    11 KB (1,018 words) - 00:47, 11 February 2009
  • Team:NTU-Singapore/Notebook/25 June 2008
    ...the 96 well into FLx800™ Fluorescence Microplate Reader and followed the protocol as before and measure RFU for 12 hrs
    3 KB (480 words) - 02:01, 28 October 2008
  • Waterloo/23 June 2008
    [x] update 1-2-3 mini prep protocol<br>
    786 B (125 words) - 15:33, 16 October 2008
  • Team:LCG-UNAM-Mexico
    var gaJsHost = (("https:" == document.location.protocol) ? "https://ssl." : "http://www.");
    9 KB (1,208 words) - 04:19, 30 October 2008
  • Team:MIT/Organizational Daily Notebook
    * [[../toothbindingassay | Tooth binding assay protocol]]
    6 KB (804 words) - 21:10, 1 August 2008
  • Team:Chiba/Study
    *[[Team:Chiba/protocol/j|具体的な実験について]]
    20 KB (1,037 words) - 11:22, 18 December 2008
  • 2008.igem.org/Team:Guelph/Project
    want to add any more genes, we use the same protocol with SwaI, StuI and
    8 KB (1,313 words) - 06:41, 30 June 2008
  • 2008.igem.org/Team:Guelph/
    want to add any more genes, we use the same protocol with SwaI, StuI and
    8 KB (1,313 words) - 06:42, 30 June 2008
  • Team:Guelph/carotenoids
    ...e all lined up properly. If we want to add any more genes, we use the same protocol with SwaI, StuI and AvrII. Since these are all plasmid based PCRs, I expect
    3 KB (508 words) - 06:51, 30 June 2008
  • Team:University of Ottawa/Sponsors
    var gaJsHost = (("https:" == document.location.protocol) ? "https://ssl." : "http://www.");
    6 KB (852 words) - 22:12, 28 October 2008
  • Alberta NINT/25 June 2008
    ...solated DNA (K102008) from 24/06/08 overnights using QIAprep spin miniprep protocol.
    1 KB (167 words) - 22:54, 11 October 2008
  • Team:NTU-Singapore/Notebook/27 June 2008
    ...the 96 well into FLx800™ Fluorescence Microplate Reader and followed the protocol as before and measure RFU for 12 hrs
    2 KB (311 words) - 02:02, 28 October 2008
  • Lab FAQs
    Please see the protocol on how to [http://www.openwetware.org/wiki/TOP10_chemically_competent_cells
    703 B (115 words) - 19:10, 10 July 2008
  • Team:Chiba/Notebook
    :*[[Team:Chiba/protocol|Protocols]]
    6 KB (723 words) - 23:59, 10 February 2009
  • Team:University of Washington/Project
    ...ol ''E. coli - S. cerevisiae'' genetic transfer, we had to first develop a protocol and demonstrate (uncontrolled) conjugative transfer between these two organ
    23 KB (3,437 words) - 04:40, 30 October 2008
  • Team:NTU-Singapore/Notebook/30 June 2008
    **This is the slight modification from the standard protocol suggested in the Newsletter Vol.1 which used supplemented M9 medium instead
    2 KB (289 words) - 02:23, 28 October 2008
  • Team:Minnesota/ProjectComparator
    '''Protocol Papers'''
    14 KB (2,203 words) - 22:49, 28 October 2008
  • Team:Waterloo/Project
    ...as a result of this signal to noise ratio (data not shown). Repetition and protocol modification is required to continue these tests. UV seemed to have little
    11 KB (1,711 words) - 04:00, 30 October 2008
  • Team:Lethbridge CCS/Project
    ... on the development and optimization of a ligase-independent cloning (LIC) protocol.
    7 KB (1,074 words) - 03:30, 30 October 2008
  • Team:NTU-Singapore/Notebook/3 July 2008
    ***New protocol for gel Runs--Post soak of gel in buffer for at least 30 mins
    2 KB (383 words) - 02:24, 28 October 2008
  • Team:KULeuven/18 July 2008
    And now for something completely different: the protocol to make competent cells was continued.
    3 KB (506 words) - 18:07, 6 October 2008
  • Team:UNIPV-Pavia/Notebook/Week5
    ...rform some ligation reaction in different conditions, looking for the best protocol.
    6 KB (877 words) - 21:25, 26 October 2008
  • Team:University of Chicago/Notebook/Dmchoi87
    [[Media:Tyrosinase-Lysate.pdf | A more complete protocol for Tyrosine-Lysate characterization]]--[[User:Dmchoi87|Dmchoi87]] 18:14, 2
    2 KB (261 words) - 18:14, 29 August 2008
  • Virginia/9 July 2008
    ...rding to the [http://openwetware.org/wiki/Knight:Restriction_Digest Knight protocol] now
    530 B (80 words) - 17:07, 9 July 2008
  • Team:University of Ottawa/9 July 2008
    ::<li>Followed quick extraction protocol.
    4 KB (587 words) - 18:32, 30 July 2008
  • Team:Hawaii/Notebook/2008-07- 9
    :* Followed protocol used for transformation of cells with BioBrick plasmids from filter
    1 KB (167 words) - 03:41, 15 July 2008
  • Team:Hawaii/Meeting/2008-07-10
    # Oligo Annealing Ligation Protocol Updates(GK, KS)
    766 B (106 words) - 08:18, 10 July 2008
  • Team:KULeuven/5 August 2008
    ...t:BBa_B0015 B0015]) were transformed again with heat shock, using the iGEM-protocol.
    3 KB (463 words) - 13:36, 7 October 2008
  • Team:Chiba/jk
    :*[[Team:Chiba/protocol/j|プロトコル]]
    4 KB (366 words) - 08:14, 14 December 2008
  • Team:Hawaii/Notebook/2008-07-16
    ...gestion, precipitated the DNA and stored it in TE in the -20C freezer. The protocol can be found [[Team:Hawaii/Initial Synth. Oligo Assembly|here]].
    1 KB (183 words) - 19:57, 17 July 2008
  • Team:Hawaii/Notebook/2008-07-10
    ...ckquote>''Looks like someone is developing a comb-less gel electrophoresis protocol.'' - NW, KLS</blockquote>
    2 KB (273 words) - 09:10, 14 July 2008
  • EPF-Lausanne/21 July 2008
    ... an other buffer(elution buffer from a miniprep kit). We followed the iGEM protocol, but this time, we incubated the DNA in the buffer for 20 minutes at 42degr
    1 KB (195 words) - 12:14, 7 August 2008
  • Team:CityColSanFrancisco/Notebook/LabBooks/Colby
    Note: 12/2006 protocol was used instead of 5/2004.
    11 KB (1,687 words) - 03:21, 22 October 2009
  • Team:Johns Hopkins/Notebook/GROUP 5: MATa Specific Promoters II
    in protocol however, so final transfer to the iGEM vector should still be straightforwa
    3 KB (495 words) - 04:54, 30 October 2008
  • Team:ESBS-Strasbourg/Plasmid urification
    b) Protocol <br>
    526 B (86 words) - 14:25, 14 July 2008
  • Team:ESBS-Strasbourg/Agarose gel preparation
    b) Protocol <br>
    513 B (72 words) - 14:27, 14 July 2008
  • Team:ESBS-Strasbourg/Dephosphorylation
    b) Protocol <br>
    209 B (34 words) - 14:30, 14 July 2008
  • Team:University of Chicago/Notebook/Shorelandhall
    Prepared 1 L SOB medium using protocol from the White Notebook. This will be used tomorrow to make electrocompeten
    1 KB (230 words) - 20:15, 28 August 2008
  • Team:NTU-Singapore/Notebook/11 July 2008
    **Two protocols were carried out, one was Drew Endy's protocol (using T4 DNA Ligase) and the other was Quick Ligase, both on E7 and T7ptag
    5 KB (837 words) - 02:26, 28 October 2008
  • EPF-Lausanne/9 July 2008
    We have prepared some buffers : SOC, LB, SOB. See the protocols in the protocol page.
    841 B (142 words) - 12:15, 7 August 2008
  • EPF-Lausanne/11 July 2008
    - We do some transformations,following the protocol of Invitrogen. :
    967 B (151 words) - 12:15, 7 August 2008
  • Team:University of Ottawa/21 July 2008
    ...d 22 July 2008, performed a transformation of competent cells according to protocol
    5 KB (811 words) - 18:30, 30 July 2008
  • EPF-Lausanne/17 July 2008
    We began a new transformation, following exactly the iGEM protocol. We took our Top10 cells, sample B. The iGEM parts used are the 4H 1018 and
    1 KB (214 words) - 12:14, 7 August 2008
  • Team:The University of Alberta/15 July 2008
    ...have arrived (minus one of them)! Siama has transformed them following the protocol supplied by the company.
    550 B (90 words) - 21:26, 16 July 2008
  • Team:University of Ottawa/15 July 2008
    ::<li> the AtCRE was extracted from the gel using the gel extraction kit and protocol
    6 KB (837 words) - 18:31, 30 July 2008
  • Team:ESBS-Strasbourg/16 July 2008
    **'''cells | part/vector | protocol''' *Miniprep with b.1) Protocol of the BBa_J63002(?) Plasmid to verify the identity by digestion after PCR
    1 KB (150 words) - 10:11, 17 July 2008
  • Team:Paris/July 13
    * Transformation of the Biobricks with the "classical" protocol (--> heat shock at 42°C during 40")
    3 KB (490 words) - 08:52, 16 July 2008
  • Team:Paris/July 14
    Hypothesis : problem with the protocol of DNA extraction from the registery 2008
    736 B (105 words) - 10:08, 16 July 2008
  • Team:KULeuven/17 July 2008
    We continued the protocol to make competent cells. The grown TOP10 cells were inoculated in SOB broth
    2 KB (307 words) - 18:06, 6 October 2008
  • Alberta NINT/11 July 2008
    Carried out Day 2 protocol for Activity Assay
    690 B (83 words) - 22:59, 11 October 2008
  • Team:Paris/July 16
    use of a new protocol --> directly competent cells
    1 KB (154 words) - 18:47, 16 July 2008
  • Team:Rice University/Electrocompetent
    ...(Cells should be kept as close to 4*C as possible for the remainder of the protocol).
    2 KB (289 words) - 19:38, 16 July 2008
  • Team:University of Ottawa/17 July 2008
    ::<li> followed transformation of competent cells protocol to insert AtCRE plasmid into preprepared competent E. coli cells.
    6 KB (862 words) - 23:46, 28 October 2008
  • Team:University of Sheffield /Project
    *41) McLean, R. J. C. et al. ('''2004'''), A simple screening protocol for the identification of quorum signal antagonists
    22 KB (3,311 words) - 01:07, 29 October 2008
  • EPF-Lausanne/18 July 2008
    ...ycerol stock and mini prep of PhD2, PhD3, PSND grown overnight, using same protocol as before(Qiagen).
    611 B (94 words) - 12:14, 7 August 2008
  • Minnesota/5 August 2008
    ...trophoresis gel and purified out using QIAGEN's QuickSpin gel purification protocol. DNA used in ligation below.
    3 KB (494 words) - 21:03, 6 August 2008
  • Team:Imperial College/CAT
    ...08.igem.org/wiki/index.php?title{{equals}}Team:Imperial_College/Resistance protocol].
    2 KB (290 words) - 02:09, 30 October 2008
  • Utah State/18 July 2008
    ...ng the Mini-Scale Preparation for DNA Sequencing by CTAB-DNA Precipitation protocol. Restriction enzyme digestion was done using Xba1 and Pst1. Electrophores
    335 B (49 words) - 05:49, 22 July 2008
  • Team:Paris/July 19
    The protocol of MiniPrep of Quiagen has been done on all the clones cultivated on the 18
    6 KB (765 words) - 12:54, 22 July 2008
  • Team:BCCS-Bristol/Calendar-Notebook/21 July 2008
    ...y.org/Part:BBa_E0240 GFP generator] and put in TE buffer (below) following protocol on iGEM sheet.
    3 KB (330 words) - 14:01, 23 September 2008
  • Virginia/21 July 2008
    *Update Protocol Page
    2 KB (287 words) - 18:03, 21 July 2008
  • Team:Johns Hopkins/Notebook/GROUP 2: MATa Specific-promoters
    have good results with regular PCR protocol (not touchdown) in which
    9 KB (1,391 words) - 04:37, 30 October 2008
  • Team:Johns Hopkins/Notebook/GROUP 1: Fluorescent Proteins
    Used digest protocol. Sequences have been made, YFP and mCherry have mutations.
    7 KB (1,009 words) - 03:32, 30 October 2008
  • Team:IIT Madras/Notebook
    Transformation protocol used.
    4 KB (661 words) - 05:20, 27 September 2008
  • Team:Paris/July 23
    *The protocol of MiniPrep of Promega has been done on all the clones cultivated on the 22
    2 KB (338 words) - 10:15, 25 August 2008
  • Team:KULeuven/24 July 2008
    In the afternoon we started the protocol to make the desired ''E.coli'' strain by transducing the EnvZ-mutation from
    6 KB (965 words) - 13:07, 7 October 2008
  • EPF-Lausanne/24 July 2008
    ...g, we prepared cells to be electrocompetent(25 aliquots). We made the same protocol as yesterday but we incubated the cells only 2:20. The OD600 was already at
    754 B (115 words) - 12:13, 7 August 2008
  • Team:University of Ottawa/Polymerase Chain Reaction
    This protocol serves as a guideline for setting-up a Polymerase Chain Reaction (PCR) for
    7 KB (1,123 words) - 00:11, 27 October 2008
  • Team:University of Ottawa/23 July 2008
    ::<li> Used minprep kit and protocol to isolate AtCRE from inoculated cells.
    5 KB (722 words) - 18:34, 7 August 2008
  • Team:Princeton/Notebook
    Each protocol performed and experiment conducted has its own entry in a lab notebook rese
    5 KB (713 words) - 03:57, 30 October 2008
  • Team:KULeuven/25 July 2008
    .... This morning we couldn't see any plaques, we still went through with the protocol. Hopefully we'll have some results on Monday.
    2 KB (311 words) - 13:07, 7 October 2008
  • Team:Paris/July 24
    *The Promega MiniPreps protocol has been used on all the clones cultivated on the 23th.
    11 KB (1,480 words) - 14:39, 31 July 2008
  • EPF-Lausanne/23 July 2008
    ...competent cells, we incubated for 4 hour our cells in SOB(according to the protocol)... But then, the OD600 was already at 1.6. We had to throw the cells away.
    650 B (105 words) - 12:13, 7 August 2008
  • Guelph/5 June 2008
    '''This was our protocol:'''
    1 KB (212 words) - 15:13, 24 July 2008
  • Minnesota/24 July 2008
    ...om gel and purified those bands (which contain the DNA wanted). Follow QIA protocol: (1) Excise the DNA fragment from the agarose gel with a clean blade, (2) P
    5 KB (821 words) - 00:17, 25 July 2008
  • EPF-Lausanne/25 July 2008
    Protocol:
    1 KB (192 words) - 12:13, 7 August 2008
  • Alberta NINT/23 July 2008
    using QIAprep spin miniprep standard protocol (to obtain more DNA for these constructs).
    968 B (108 words) - 23:02, 11 October 2008
  • Team:University of Chicago/Notebook/Buffers
    ** Previous protocol indicated amount of acetic acid added should be 23 ml/liter but that amount
    2 KB (292 words) - 21:57, 5 August 2008
  • Team:Hawaii/Ligation of pRL1383a Parts
    :*Protocol:
    21 KB (3,321 words) - 03:32, 30 October 2008
  • Team:Tokyo Tech
    !align="center" width=20% style="text-align:center"|[[Team:Tokyo_Tech/Protocol|Protcol]]
    16 KB (2,499 words) - 05:25, 30 October 2008
  • TUDelft/28 August 2008
    ...one with ''Eco''RI and ''Spe''I from NEB, in a ''Eco''RI buffer, using the protocol of iGEM-MIT. The result is in the gel below.
    849 B (128 words) - 09:37, 27 October 2008
  • Team:Tokyo Tech/Team
    !align="center" width=20% style="text-align:center"|[[Team:Tokyo_Tech/Protocol|Protcol]]
    2 KB (267 words) - 06:27, 30 October 2008
  • Wisconsin: Lignin Project/3 July 2008
    To do this we followed the protocol of the Weibel Lab.<br>
    982 B (156 words) - 02:40, 29 October 2008
  • Team:Hawaii/Construction of Broad-Host-Range Expression Vector
    ...Oligonucleotide_Inserts]] Silver Lab, Overlapping Oligonucleotides Inserts Protocol.
    5 KB (667 words) - 01:10, 16 August 2008
  • Team:Tokyo Tech/Parts
    !align="center" width=20% style="text-align:center"|[[Team:Tokyo_Tech/Protocol|Protcol]]
    2 KB (241 words) - 21:49, 29 October 2008
  • Team:Tokyo Tech/Acknowledgements
    !align="center" width=20% style="text-align:center"|[[Team:Tokyo_Tech/Protocol|Protcol]]
    4 KB (502 words) - 05:20, 30 October 2008
  • Team:University of Ottawa/1 August 2008
    :<li> Microwave protocol is therefore not useful for time frames tested. However, current digestion
    6 KB (957 words) - 15:10, 12 August 2008
  • Team:Paris/Modeling/estimation
    ...the followings. They are linked to a basic description of the "theoretical protocol" that will allow us to get the expected datas. We decided, as far as possib
    12 KB (1,883 words) - 07:07, 30 October 2008
  • Alberta NINT/30 July 2008
    Extracted DNA from gel using QIAquick gel extraction protocol.
    604 B (77 words) - 23:05, 11 October 2008
  • Alberta NINT/6 August 2008
    ... Carried out calculations to better determine appropriate dilutions and protocol for annealing,
    479 B (58 words) - 23:06, 11 October 2008
  • Team:The University of Alberta/7 August 2008
    *PCRed PCAM2201 using Cam35s Forward and Reverse Primers. (Protocol to be followed is given in the Masterbook)
    1,014 B (174 words) - 16:38, 11 August 2008
  • Team:University of Ottawa/5 August 2008
    :<li> Digestion was performed according to previous protocol, including denaturation at 80&deg;C and addition of ligase+ ATP.
    6 KB (918 words) - 19:02, 7 August 2008
  • Team:University of Ottawa/7 August 2008
    :<li> Digested all three according to previous digestion protocol (see August 5 post). Ran 7 samples--six ligations and one digestion product
    5 KB (748 words) - 15:24, 12 August 2008
  • Newcastle University Wetlab/9 September 2008
    * Glycerol stocks made (see protocol) of iGEMgfp 7.1 and 11.1 and iGEMcherry 13.1 and 13.2.
    1 KB (171 words) - 18:57, 21 September 2008
  • Team: University of Chicago/Notebook/SDS-PAGE
    SDS-PAGE Protocol <<Protocol acquired from https://2007.igem.org/Melbourne/SDS_PAGE>>
    1 KB (179 words) - 21:09, 12 September 2008
  • Team:Paris/Notebook/Protocols
    = Protocol to make competent bacteria =
    16 KB (2,518 words) - 12:45, 21 October 2008
  • Team:Mississippi State/4 August 2008
    # Repeat digestion protocol using NotI, EcoRI, LiP, and pPIC6
    390 B (59 words) - 19:19, 11 August 2008
  • Team:KULeuven/12 August 2008
    The PCR of the T7 polymerase was continued. We tried the same protocol as yesterday, but this time with a longer amplification time, but it failed
    4 KB (564 words) - 12:29, 11 October 2008
  • TUDelft/17 July 2008
    ...e IPTG GFP construct from the iGEM spots. This was done to test extraction protocol and transformation. Protocols followed were those on OpenWetWare. Cells wer
    429 B (53 words) - 11:17, 4 September 2008

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